Mucus accumulation in the lungs precedes structural changes and infection in children with cystic fibrosis

Although destructive airway disease is evident in young children with cystic fibrosis (CF), little is known about the nature of the early CF lung environment triggering the disease. To elucidate early CF pulmonary pathophysiology, we performed mucus, inflammation, metabolomic, and microbiome analyses on bronchoalveolar lavage fluid (BALF) from 46 preschool children with CF enrolled in the Australian Respiratory Early Surveillance Team for Cystic Fibrosis (AREST CF) program and 16 non-CF disease controls. Total airway mucins were elevated in CF compared to non-CF BALF irrespective of infection, and higher densities of mucus flakes containing mucin 5B and mucin 5AC were observed in samples from CF patients. Total mucins and mucus flakes correlated with inflammation, hypoxia, and oxidative stress. Many CF BALFs appeared sterile by culture and molecular analyses, whereas other samples exhibiting bacterial taxa associated with the oral cavity. Children without computed tomography–defined structural lung disease exhibited elevated BALF mucus flakes and neutrophils, but little/no bacterial infection. Although CF mucus flakes appeared “permanent” because they did not dissolve in dilute BALF matrix, they could be solubi-lized by a previously unidentified reducing agent (P2062), but not N-acetylcysteine or deoxyribonuclease. These findings indicate that early CF lung disease is characterized by an increased mucus burden and inflammatory markers without infection or structural lung disease and suggest that mucolytic and anti-inflammatory agents should be explored as preventive therapy

Effects of watering treatments on germination, survival, and growth of Rocky Mountain Douglas Fir : a greenhouse study.

RM-RN-35

Radio frequency wave experiments on the MST reversed field pinch

Experiments, simulations, and theory all indicate that the magnetic fluctuations responsible for the poor confinement in the reversed field pinch (RFP) can be controlled by altering the radial profile of the current density. The magnetic fluctuations in the RFP are due to resistive MHD instabilities caused by current profile peaking; thus confinement in the RFP is ultimately the result of a misalignment between inductively driven current profiles and the stable current profiles characteristic of the Taylor state. If a technique such as rf current drive can be developed to non-inductively sustain a Taylor state (a current profile linearly stable to all tearing modes), the confinement of the RFP and its potential as a reactor concept are likely to increase. Whether there is a self-consistent path from poor confinement to greatly improved confinement through current profile modification is an issue for future experiments to address if and only if near term experiments can demonstrate: (1) coupling to and the propagation of rf waves in RFP plasmas, (2) efficient current drive, and (3) control of the power deposition which will make it possible to control the current profile. In this paper, modeling results and experimental plans are presented for two rf experiments which have the potential of satisfying these three goals: high-n{sub {parallel}} lower hybrid (LH) waves and electron Bernstein waves (EBWs)

Bamboos : current research; proceedings of the International Bamboo Workshop

Meeting: International Bamboo Workshop, 14-18 Nov. 1988, Cochin, INPublished jointly with IDR