Tight-binding parameters for charge transfer along DNA

We systematically examine all the tight-binding parameters pertinent to charge transfer along DNA. The $\pi$ molecular structure of the four DNA bases (adenine, thymine, cytosine, and guanine) is investigated by using the linear combination of atomic orbitals method with a recently introduced parametrization. The HOMO and LUMO wavefunctions and energies of DNA bases are discussed and then used for calculating the corresponding wavefunctions of the two B-DNA base-pairs (adenine-thymine and guanine-cytosine). The obtained HOMO and LUMO energies of the bases are in good agreement with available experimental values. Our results are then used for estimating the complete set of charge transfer parameters between neighboring bases and also between successive base-pairs, considering all possible combinations between them, for both electrons and holes. The calculated microscopic quantities can be used in mesoscopic theoretical models of electron or hole transfer along the DNA double helix, as they provide the necessary parameters for a tight-binding phenomenological description based on the $\pi$ molecular overlap. We find that usually the hopping parameters for holes are higher in magnitude compared to the ones for electrons, which probably indicates that hole transport along DNA is more favorable than electron transport. Our findings are also compared with existing calculations from first principles.Comment: 15 pages, 3 figures, 7 table

Bio-based plastics in durable applications: The future of sustainable product design? A design review

This design review evaluates the use of bio-based plastics in durable consumer products. The main question is: how does the use of bio-based plastics influence the product’s design, functionality, marketing & communication, and sustainability? Although higher material prices would lead one to expect that higher value applications would be targeted, research shows bio-based plastics are mainly used in short-lived applications like packaging. This study investigates their use in durable consumer products through a design review. The results indicate that bio-based plastic usage is still in its early days in durable products. Bio-based plastics appear to be utilized as straightforward replacement of fossil-based plastic. Designers are not yet using the unique properties of bio-based plastics in the design of their products. Companies mainly exploit the green image of bio-based plastics in their marketing & communication. Their focus is on the renewable feedstock and not on sourcing, or on recovery at end-of-life.Circular Product DesignDesign for Sustainabilit

Beyond flight operations: Assessing the environmental impact of aircraft maintenance through life cycle assessment

As the aviation industry strives to minimise its environmental footprint, understanding the full life cycle impacts, including maintenance, becomes essential for sustainable development. This paper addresses the critical research gap in the environmental assessment of aircraft maintenance by conducting a comprehensive life cycle assessment based on an Airbus A320 aircraft. By combining a top-down check-level analysis and a detailed examination of the aircraft manufacturer's maintenance planning document, this study provides significant insights into the environmental implications of maintenance activities. The check-level analysis provides a general overview, while the analysis of the maintenance planning document delves into individual tasks, enabling the identification of components with the highest ecological impacts. This research emphasises the importance of including aircraft maintenance activities in life cycle assessment studies and provides valuable guidance for researchers, industry practitioners, and policy makers in prioritising sustainability measures and enhancing the environmental performance of aircraft throughout their life cycle.Industrial Design EngineeringDesign for SustainabilityGroup Dransfel

Design of a syringe extension device (Chloe SED®) for low-resource settings in sub-Saharan Africa: a circular economy approach

Underfunded healthcare infrastructures in low-resource settings in sub-Saharan Africa have resulted in a lack of medical devices crucial to provide healthcare for all. A representative example of this scenario is medical devices to administer paracervical blocks during gynaecological procedures. Devices needed for this procedure are usually unavailable or expensive. Without these devices, providing paracervical blocks for women in need is impossible resulting in compromising the quality of care for women requiring gynaecological procedures such as loop electrosurgical excision, treatment of miscarriage, or incomplete abortion. In that perspective, interventions that can be integrated into the healthcare system in low-resource settings to provide women needing paracervical blocks remain urgent. Based on a context-specific approach while leveraging circular economy design principles, this research catalogues the development of a new medical device called Chloe SED® that can be used to support the provision of paracervical blocks. Chloe SED®, priced at US$1.5 per device when produced in polypropylene, US$ 10 in polyetheretherketone, and US$ 15 in aluminium, is attached to any 10-cc syringe in low-resource settings to provide paracervical blocks. The device is designed for durability, repairability, maintainability, upgradeability, and recyclability to address environmental sustainability issues in the healthcare domain. Achieving the design of Chloe SED® from a context-specific and circular economy approach revealed correlations between the material choice to manufacture the device, the device's initial cost, product durability and reuse cycle, reprocessing method and cost, and environmental impact. These correlations can be seen as interconnected conflicting or divergent trade-offs that need to be continually assessed to deliver a medical device that provides healthcare for all with limited environmental impact. The study findings are intended to be seen as efforts to make available medical devices to support women's access to reproductive health services.Design for SustainabilityMarketing and Consumer ResearchCircular Product Desig

Identification and expression of the first nonmammalian amyloid-beta precursor-like protein APLP2 in the amphibian Xenopus laevis.

Contains fulltext : 58232.pdf (publisher's version ) (Closed access)The Alzheimer's disease-linked amyloid-beta precursor protein (APP) belongs to a superfamily of proteins, which also comprises the amyloid-beta precursor-like proteins, APLP1 and APLP2. Whereas APP has been identified in both lower and higher vertebrates, thus far, APLP1 and 2 have been characterized only in human and rodents. Here we identify the first nonmammalian APLP2 protein in the South African claw-toed frog Xenopus laevis. The identity between the Xenopus and mammalian APLP2 proteins is approximately 75%, with the highest degree of conservation in a number of amino-terminal regions, the transmembrane domain and the cytoplasmic tail. Furthermore, amino acid residues known to be phosphorylated and glycosylated in mammalian APLP2 are conserved in Xenopus. The availability of the Xenopus APLP2 protein sequence allowed a phylogenetic analysis of APP superfamily members that suggested the occurrence of APP and preAPLP lineages with their separation predating the mammalian-amphibian split. As in mammals, Xenopus APLP2 mRNA was ubiquitously expressed and alternatively spliced forms were detected. However, the expression ratios between the mRNA forms in the various tissues examined were different between Xenopus and mammals, most prominently for the alternatively spliced forms containing the Kunitz protease inhibitor-coding region that were less abundantly expressed than the corresponding mammalian forms. Thus, the identification of APLP2 in Xenopus has revealed evolutionarily conserved regions that may help to delineate functionally important domains, and its overall high degree of conservation suggests an important role for this APP superfamily member