Challenges and Opportunities in Rural Nursing Preceptorship: What Multimedia Participant Action Reveals (Défis et possibilités du préceptorat infirmier en milieu rural : ce qu’une participation multimédia révèle)

Background: Rural health care sites struggle to attract new nurses, owing to a widespread perception that the hardships of rural practice far outweigh the benefits. Preceptorships are a key means of recruiting nursing staff to rural locations, but innovative, firsthand messaging is needed to promote rural preceptorships and nursing careers. Objectives: The researchers sought to elicit compelling, multimedia, firsthand accounts of the challenges and opportunities of rural preceptorship through participant action. Additional goals were to explore the ways in which participants reify their experiences through digital media, and the potential for digitally-based participant action research to empower participants. Methods: The study was designed to engage participants in all phases of research: data collection, analysis, and dissemination of findings. It comprised three phases, each employing participant action methodology: photovoice data collection, collaborative thematic analysis, and authorship of digital stories. Participants: Through purposive and snowball sampling, the researchers recruited seven nursing students and five rural, registered nurses assigned to precept them. Inclusion criteria for the students were enrolment in the senior (final) preceptorship course prior to graduation, and the choice of a rural, semirural or suburban site. No exclusion criteria were warranted owing to the limited cohort of participants. Settings: Data were collected at six acute care sites and one community care site. The sites were rural, semi-rural and suburban, serving populations ranging from 800 to 18,000, between 42 km and 416 km distant from the students’ primary place of study. Results: It was found that rural preceptorships teach students to accept and manage limitations, while appreciating and capitalizing on opportunities; this finding was equally true for nominally suburban and semi-rural sites included in the study. Emerging from the interviews, challenges, being more concrete, were reflected in photographic data, while opportunities were more abstract and relational. Citing time constraints, most participants declined to author their own digital stories. Conclusions: Digitally-based participant action enables nurse preceptors and their students to make a compelling case for rural preceptorships and rural careers. However, digital media may also distort these participants’ experiences, and their involvement in all phases of research may be more burdensome than empowering. Résumé Contexte : Les établissements de soins de santé ruraux peinent à attirer de nouvelles infirmières en raison d’une perception répandue voulant que les inconvénients de la pratique en milieu rural dépassent de loin ses avantages. Si les stages avec préceptorat représentent un moyen essentiel pour recruter de nouvelles infirmières en milieu rural, un message novateur, à partir de l’expérience vécue, est nécessaire pour promouvoir le préceptorat et la carrière dans ce milieu. Objectifs : L’étude avait pour but de recueillir des témoignages éloquents, livrés directement par les participantes au moyen de divers médias, sur les défis et les possibilités du préceptorat en milieu rural, selon une approche participative. Elle visait également à explorer les méthodes utilisées par les participantes pour rendre leurs expériences sur un support numérique, ainsi que le potentiel d’habilitation d’une recherche participative par voie numérique pour les participantes. Méthodologie : Le devis de l’étude favorisait l’engagement des participantes dans toutes les phases de la recherche, soit la collecte et l’analyse des données, ainsi que la diffusion des résultats. Elle comprenait trois phases, chacune étant menée selon l’approche de recherche participative : collecte de données par la méthode Photovoice, analyse thématique collaborative et rédaction comme auteures des récits numériques. Participantes : Par échantillonnage intentionnel et boule de neige, les chercheuses ont recruté sept étudiantes en sciences infirmières et leurs préceptrices, soit cinq infirmières pratiquant en milieu rural. Les critères d’inclusion des étudiantes comprenaient l’inscription au dernier stage avec préceptorat avant l’obtention du diplôme, ainsi que la sélection d’un établissement rural, semi-rural ou suburbain. Compte tenu du caractère limité de la cohorte de participantes, les chercheuses n’ont retenu aucun critère d’exclusion. Milieux : La cueillette des données s’est déroulée dans six établissements de soins de courte durée et un établissement de soins communautaires. Ces sites desservaient de 800 à 18 000 personnes et étaient établis en zone rurale, semi-rurale et suburbaine, de 42 à 416 km de l’établissement d’enseignement principal des étudiantes. Résultats : L’étude indique que le préceptorat rural apprend aux étudiantes à accepter et à gérer les contraintes tout en appréciant et en exploitant les possibilités; ce résultat s’est aussi avéré pour les emplacements suburbains et semi-ruraux inclus dans l’étude. Les défis soulevés dans les entrevues, plutôt concrets, ont été illustrés par les données photographiques, alors que les possibilités identifiées étaient de nature abstraite et relationnelle. Évoquant la contrainte de temps, la plupart des participantes ont décliné la rédaction de leur propre récit numérique. Conclusions : La recherche participative par voie numérique permet aux infirmières préceptrices et à leurs étudiantes de livrer un vibrant plaidoyer en faveur du préceptorat et de la carrière en milieu rural. Cependant, les médias numériques peuvent aussi altérer l’expérience des participantes, et leur implication dans toutes les phases de la recherche peut constituer un fardeau plutôt qu’un facteur d’habilitation

“Tales and Adventures”: G.A. Henty’s Union Jack and the Competitive World of Publishing for Boys in the 1880s’

In the competitive publishing environment of the late nineteenth century, writers and magazines had to distinguish themselves carefully from potential rivals. This article examines how G.A. Henty’s quality boys’ weekly, Union Jack (1880-83), attempted to secure a niche in the juvenile publishing market by deliberately distinguishing itself from other papers as a literary, imperialist and “healthy” publication. The article explores the design and marketing techniques of the magazine, its status as a fiction paper, the high calibre of its contributors, and its aggressive rhetoric in targeting an exclusively masculine audience. It argues that while Union Jack was marketed as a niche publication, it eventually failed to distinguish itself sufficiently to survive in an extremely competitive environment

Reactivation of Fault Systems by Compartmentalized Hydrothermal Fluids in the Southern Andes Revealed by Magnetotelluric and Seismic Data

In active volcanic arcs such as the Andean volcanic mountain belt, magmatically‐sourced fluids are channelled through the brittle crust by faults and fracture networks. In the Andes, volcanoes, geothermal springs and major mineral deposits have a spatial and genetic relationship with NNE‐trending, margin‐parallel faults and margin‐oblique, NW‐trending Andean Transverse Faults (ATF). The Tinguiririca and Planchón‐Peteroa volcanoes in the Andean Southern Volcanic Zone (SVZ) demonstrate this relationship, as their spatially associated thermal springs show strike alignment to the NNE‐oriented El Fierro Thrust Fault System. We constrain the fault system architecture and its interaction with volcanically sourced hydrothermal fluids using a combined magnetotelluric (MT) and seismic survey that was deployed for 20 months. High conductivity zones are located along the axis of the active volcanic chain, delineating fluids and/or melt. A distinct WNW‐trending cluster of seismicity correlates with resistivity contrasts, considered to be a reactivated ATF. Seismicity occurs below 4 km, suggesting activity is limited to basement rocks, and the cessation of seismicity at 9 km delineates the local brittle‐ductile transition. As seismicity is not seen west of the El Fierro fault, we hypothesize that this structure plays a key role in compartmentalizing magmatically‐derived hydrothermal fluids to the east, where the fault zone acts as a barrier to cross‐fault fluid migration and channels fault‐parallel fluid flow to the surface from depth. Increases in fluid pressure above hydrostatic may facilitate reactivation. This site‐specific case study provides the first three‐dimensional seismic and magnetotelluric observations of the mechanics behind the reactivation of an ATF

Deciphering mollusc shell production: the roles of genetic mechanisms through to ecology, aquaculture and biomimetics

Most molluscs possess shells, constructed from a vast array of microstructures and architectures. The fully formed shell is composed of calcite or aragonite. These CaCO3 crystals form complex biocomposites with proteins, which although typically less than 5% of total shell mass, play significant roles in determining shell microstructure. Despite much research effort, large knowledge gaps remain in how molluscs construct and maintain their shells, and how they produce such a great diversity of forms. Here we synthesize results on how shell shape, microstructure, composition and organic content vary among, and within, species in response to numerous biotic and abiotic factors. At the local level, temperature, food supply and predation cues significantly affect shell morphology, whilst salinity has a much stronger influence across latitudes. Moreover, we emphasize how advances in genomic technologies [e.g. restriction site-associated DNA sequencing (RAD-Seq) and epigenetics] allow detailed examinations of whether morphological changes result from phenotypic plasticity or genetic adaptation, or a combination of these. RAD-Seq has already identified single nucleotide polymorphisms associated with temperature and aquaculture practices, whilst epigenetic processes have been shown significantly to modify shell construction to local conditions in, for example, Antarctica and New Zealand. We also synthesize results on the costs of shell construction and explore how these affect energetic trade-offs in animal metabolism. The cellular costs are still debated, with CaCO3 precipitation estimates ranging from 1-2 J/mg to 17-55 J/mg depending on experimental and environmental conditions. However, organic components are more expensive (~29 J/mg) and recent data indicate transmembrane calcium ion transporters can involve considerable costs. This review emphasizes the role that molecular analyses have played in demonstrating multiple evolutionary origins of biomineralization genes. Although these are characterized by lineage-specific proteins and unique combinations of co-opted genes, a small set of protein domains have been identified as a conserved biomineralization tool box. We further highlight the use of sequence data sets in providing candidate genes for in situ localization and protein function studies. The former has elucidated gene expression modularity in mantle tissue, improving understanding of the diversity of shell morphology synthesis. RNA interference (RNAi) and clustered regularly interspersed short palindromic repeats - CRISPR-associated protein 9 (CRISPR-Cas9) experiments have provided proof of concept for use in the functional investigation of mollusc gene sequences, showing for example that Pif (aragonite-binding) protein plays a significant role in structured nacre crystal growth and that the Lsdia1 gene sets shell chirality in Lymnaea stagnalis. Much research has focused on the impacts of ocean acidification on molluscs. Initial studies were predominantly pessimistic for future molluscan biodiversity. However, more sophisticated experiments incorporating selective breeding and multiple generations are identifying subtle effects and that variability within mollusc genomes has potential for adaption to future conditions. Furthermore, we highlight recent historical studies based on museum collections that demonstrate a greater resilience of molluscs to climate change compared with experimental data. The future of mollusc research lies not solely with ecological investigations into biodiversity, and this review synthesizes knowledge across disciplines to understand biomineralization. It spans research ranging from evolution and development, through predictions of biodiversity prospects and future-proofing of aquaculture to identifying new biomimetic opportunities and societal benefits from recycling shell products.FCT: UID/Multi/04326/2019; European Marine Biological Research Infrastructure Cluster-EMBRIC (EU H2020 research and innovation program) 654008; European Union Seventh Framework Programme [FP7] ITN project 'CACHE: Calcium in a Changing Environment' under REA 60505; NERC Natural Environment Research Council NE/J500173/1info:eu-repo/semantics/publishedVersio

Host-specific pit-forming epizoans on Silurian crinoids

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/75404/1/j.1502-3931.1978.tb01229.x.pd

Circadian Clock Gene Expression in the Coral Favia fragum over Diel and Lunar Reproductive Cycles

Natural light cycles synchronize behavioral and physiological cycles over varying time periods in both plants and animals. Many scleractinian corals exhibit diel cycles of polyp expansion and contraction entrained by diel sunlight patterns, and monthly cycles of spawning or planulation that correspond to lunar moonlight cycles. The molecular mechanisms for regulating such cycles are poorly understood. In this study, we identified four molecular clock genes (cry1, cry2, clock and cycle) in the scleractinian coral, Favia fragum, and investigated patterns of gene expression hypothesized to be involved in the corals' diel polyp behavior and lunar reproductive cycles. Using quantitative PCR, we measured fluctuations in expression of these clock genes over both diel and monthly spawning timeframes. Additionally, we assayed gene expression and polyp expansion-contraction behavior in experimental corals in normal light:dark (control) or constant dark treatments. Well-defined and reproducible diel patterns in cry1, cry2, and clock expression were observed in both field-collected and the experimental colonies maintained under control light:dark conditions, but no pattern was observed for cycle. Colonies in the control light:dark treatment also displayed diel rhythms of tentacle expansion and contraction. Experimental colonies in the constant dark treatment lost diel patterns in cry1, cry2, and clock expression and displayed a diminished and less synchronous pattern of tentacle expansion and contraction. We observed no pattern in cry1, cry2, clock, or cycle expression correlated with monthly spawning events suggesting these genes are not involved in the entrainment of reproductive cycles to lunar light cycles in F. fragum. Our results suggest a molecular clock mechanism, potentially similar to that in described in fruit flies, exists within F. fragum