Effect of stocking density of fish on water quality and growth performance of European Carp and leafy vegetables in a low-tech aquaponic system

Aquaponics (AP) is a semi-closed system of food production that combines aquaculture and hydroponics and represents a new agricultural system integrating producers and consumers. The aim of this study was to test the effect of stocking densities (APL, 2.5 kg m-3; APH, 4.6 kg m-3) on water quality, growth performance of the European Carp (Cyprinus carpio L.), and yield of leafy vegetables (catalogna, lettuce, and Swiss Chard) in a low-technology AP pilot system compared to a hydroponic cultivation. The AP daily consumption of water due to evapotranspiration was not different among treatments with an average value of 8.2 L d-1, equal to 1.37% of the total water content of the system. Dissolved oxygen was significantly (p < 0.05) different among treatments with the lowest median value recorded with the highest stocking density of fish (5.6 mg L-1) and the highest median value in the hydroponic control (8.7 mg L-1). Marketable yield of the vegetables was significantly different among treatments with the highest production in the hydroponic control for catalogna (1.2 kg m-2) and in the APL treatment for Swiss Chard (5.3 kg m-2). The yield of lettuce did not differ significantly between hydroponic control and APL system (4.0 kg m-2 on average). The lowest production of vegetables was obtained in the APH system. The final weight (515 g vs. 413 g for APL and APH, respectively), specific growth rate (0.79% d-1 vs. 0.68% d-1), and feed conversion (1.55 vs. 1.86) of European Carp decreased when stocking density increased, whereas total yield of biomass was higher in the APH system (4.45 kg m-3 vs. 6.88 kg m-3). A low mortality (3% on average) was observed in both AP treatments. Overall, the results showed that a low initial stocking density at 2.5 kg m-3 improved the production of European Carp and of leafy vegetables by maintaining a better water quality in the tested AP system

Influence of group housing, lactation system and floor type on performance and injuries in rabbit does

To evaluate the effect of group housing, lactation system, and floor type, 40 crossbred multiparous pregnant rabbit does were transferred to individual pens (0.5 m x 1.0 m); 8 does were kept individually during all the trial (I; n=8); the other 32 does were kept in 8 collective pens (2.0 m x 1.0 m, by joining 4 individual pens) in stable groups of 4 individuals (G; n=8) from 8 d until 2 d before kindling and from 2 d until 33 d after kindling. Half pens had wire net floor covered with plastic mats (W; n=8) and the other half plastic slatted floor (P; n=8). Within G does, in half pens (n=4) each doe nursed its own litter (fixed lactation, F); in the remaining half, each doe randomly nursed the litters of the group (random lactation, R). Doe performance at kindling and during lactation, litter performance from standardization until weaning (33 d), and skin injuries on G does (5, 12, 19, 26 and 34 d after kindling) were controlled. The effect of the housing system on performance was tested by PROC GLM of SAS. Within G does, the effect of the lactation system and floor type was evaluated by PROC MIXED (pen as random effect); the injury frequency was analysed by PROC FREQ according to observation day, lactation system, and floor type. Housing system did not affect doe kindling or lactation performance; litter weight at standardization was higher for G than I does (680 vs 619 g, P<0.05), but differences disappeared at weaning. The lactation system showed some residual effects when controlled lactation finished, nests were opened, and kits free of moving. In fact, from 19 to 33 d after kindling, does and litters had higher feed intake (679 g/d vs 602 g/d; P<0.05) and, at 33 d, kits were heavier (775 vs 748 g, P<0.05) in R than in F pens. Floor type (W vs P pens) affected milk production (244 vs 225 g/d; P=0.06), and kit weight at the end of controlled lactation (301 vs 290 g; P<0.05), without residual effects at weaning. The injury frequency changed after the group formation: 34% at 5 d, 47% at 12 d, 13% at 19 and 26 d, and 10% at 34 d of lactation (P<0.05). In conclusion, under our conditions, group housing did not negatively affect doe performance; lactation system and floor type had minor effects; aggressiveness among does was evident after group formation during early lactation but decreased in the late lactation. Further recordings on more reproductive cycles would be necessary to confirm the present results

Crossbreeding to improve local chicken breeds: predicting growth performance of the crosses using the Gompertz model and estimated heterosis

The present study aims to validate the Gompertz model to predict the growth performance of chicken crosses according to growth curve parameters of the parental lines and the estimated heterosis for each curve parameter. A total of 252 one-day-old chicks of both sexes belonging to 6 genotypes, including Ross 308, Sasso (SA), Bionda Piemontese (BP), and Robusta Maculata (RM), and the crosses between these local breeds and SA (BP x SA and RM x SA) were randomly allocated in 18 pens (3 pens/genotype) in mixed-sex groups (14 animals/pen; 7 females and 7 males). The individual body weight (BW) of all birds was recorded once a week from hatching until slaughtering (81 d for Ross 308; 112 d for SA, 140 d for the other genotypes). We drew up our final dataset with 240 birds (40 birds/ genotype; 20 females and 20 males). The growth curve of each genotype was described using the Gompertz model, and the heterosis for each growth curve parameter was calculated as the difference between F-1 crosses and the average of parental breeds. The predicted growth curve parameters were evaluated by cross-validation. The Gompertz model accurately estimated the growth curves of all the genotypes (R-2 > 0.90). Heterosis was significant for almost all growth curve parameters in both crosses (P < 0.05). Heterosis ranged from -13.0 to +11.5%, depending on parameters, but varied slightly between the crossbreeds (BP x SA and RM x SA). The predicted values of adult BW, weight at the inflection point, and maximum growth rate were overestimated for BP x SA and under-estimated for RM x SA, with a mean error between observed and predicted values <12.71% for all the curve parameters. In conclusion, the growth performance of chicken crosses between local breeds and commercial strains can be accurately predicted with Gompertz parameters of the parental lines adjusting for heterosis

Evaluation of carcass and meat traits of Muscovy duck fed with black soldier fly partially defatted meal

The aim of this study was to evaluate the carcass characteristics and breast meat quality in Muscovy duck (Cairina moschata domestica) fed different inclusion levels of a partially defatted black soldier fly larva (BSF) meal. A total of 256 Muscovy ducklings (average live weight, LW: 71.32\ub12.70 g) were reared from day 3 to day 48 and randomly allotted in 32 pens (8 replicates/treatment). Four different diets were formulated with increasing substitution level of corn gluten meal with BSF larva meal (0, 3, 6 and 9%; BSF0, BSF3, BSF6 and BSF9, respectively) and divided in 3 feeding phases: starter (1-14 days), grower (14-35 days) and finisher (35-48 days). At day 48, 2 animals/replicate were slaughtered and dissected to determine their carcass yields. The weights of spleen, bursa of Fabricius, liver, heart and abdominal fat were recorded. Breast and thigh muscles were then excised from 16 ducks/treatment and weighted. Ultimate pH (pHu) and L*, a*, b* colour values were then measured on breast muscle. The collected data were tested by means of oneway ANOVA evaluating the effect of dietary BSF inclusion level by polynomial contrasts. Significance was declared at P<0.05. The inclusion of BSF did not affect final LW (2,515.68\ub192.42 g on average). Hot and cold carcass weights showed a quadratic response (P<0.05) to increasing BSF larva meal, with a minimum corresponding to BSF6; however, refrigeration losses were not affected by treatments. Weight of spleen, bursa of Fabricius, liver and heart did not differ among treatments. The weight of abdominal fat showed a quadratic response to increasing BSF meal with a minimum corresponding to BSF6 group (P<0.05). Breast and thigh yields, pHu and L*, a*, b* colour values did not differ among groups. With the exception of BSF6, the inclusion of BSF meal did not affect meat traits and carcass characteristics, confirming the potential use of BSF meal in Muscovy duck diets

The use of environmental enrichments affects performance and behavior of growing rabbits housed in collective pens

This study assessed the effects of an elevated plastic-slatted platform and/or a plastic hiding tube in collective pens with large group sizes (27 or 36 rabbits/pen; 16 rabbits/m2) on the performance and welfare of rabbits kept from weaning (at 33 days of age) to slaughter (at 68 or 75 days of age). Growth performance, injuries, and behavior (video recorded for 24 h) of rabbits (n = 504) were recorded. The platform allowed rabbits to adopt the rearing position more frequently (+0.14 events during 2 min every 30 min across 24 h) and to rest with stretched body for longer (+3.8% of observed time) (p < 0.001). Production parameters and reactivity at the open field test were not modified, but the occurrence of injured rabbits at the trial end was higher in pens with platforms (+8.9%; p < 0.01). This result was possibly related to the higher group size in pens with platforms (36 rabbits) compared to those without platforms (27 rabbits). The inclusion of the tube decreased growth (-2.2 g/d; p < 0.05), whereas it was scarcely used by rabbits and it did not substantially change their behavior or the occurrence of injuries. In conclusion, under the experimental conditions of this study, elevated platforms worked as a useful structural enrichment in view of animal behavior but negatively impacted on the rate of injuries, whereas the usefulness of the tube was not confirmed

A deep-learning sequence-based method to predict protein stability changes upon genetic variations

Several studies have linked disruptions of protein stability and its normal functions to disease. Therefore, during the last few decades, many tools have been developed to predict the free energy changes upon protein residue variations. Most of these methods require both sequence and structure information to obtain reliable predictions. However, the lower number of protein structures available with respect to their sequences, due to experimental issues, drastically limits the application of these tools. In addition, current methodologies ignore the antisymmetric property characterizing the thermodynamics of the protein stability: a variation from wild-type to a mutated form of the protein structure (XW→XM) and its reverse process (XM→XW) must have opposite values of the free energy difference (ΔΔGWM=−ΔΔGMW). Here we propose ACDC-NN-Seq, a deep neural network system that exploits the sequence information and is able to incorporate into its architecture the antisymmetry property. To our knowledge, this is the first convolutional neural network to predict protein stability changes relying solely on the protein sequence. We show that ACDC-NN-Seq compares favorably with the existing sequence-based methods

W-F substitutions in apomyoglobin increase the local flexibility of the N-terminal region causing amyloid aggregation: A H/D exchange study.

Myoglobin is an α-helical globular protein containing two highly conserved tryptophanyl residues at positions 7 and 14 in the N-terminal region. The simultaneous substitution of the two residues impairs the productive folding of the protein making the polypeptide chain highly prone to aggregate forming amyloid fibrils at physiological pH and room temperature. The role played by tryptophanyl residues in driving the productive folding process was investigated by providing structural details at low resolution of compact intermediate of three mutated apomyoglobins, i.e., W7F, W14F and the amyloid forming mutant W7FW14F. In particular, we followed the hydrogen/deuterium exchange rate of protein segments using proteolysis with pepsin followed by mass spectrometry analysis. The results revealed significant differences in the N-terminal region, consisting in an alteration of the physico-chemical properties of the 7-11 segment for W7F and in an increase of local flexibility of the 12-29 segment for W14F. In the double trypthophanyl substituted mutant, these effects are additive and impair the formation of native-like contacts and favour inter-chain interactions leading to protein aggregation and amyloid formation at physiological pH