Observational Constraints on Transverse Gravity: a Generalization of Unimodular Gravity

We explore the hypothesis that the set of symmetries enjoyed by the theory that describes gravity is not the full group of diffeomorphisms Diff(M), as in General Relativity, but a maximal subgroup of it, TransverseDiff(M), with its elements having a jacobian equal to unity; at the infinitesimal level, the parameter describing the coordinate change, xi^mu (x), is transverse, i.e., partial_mu(xi^mu)=0. Incidentally, this is the smaller symmetry one needs to propagate consistently a graviton, which is a great theoretical motivation for considering these theories. Also, the determinant of the metric, g, behaves as a "transverse scalar", so that these theories can be seen as a generalization of the better-known unimodular gravity. We present our results on the observational constraints on transverse gravity, in close relation with the claim of equivalence with general scalar-tensor theory. We also comment on the structure of the divergences of the quantum theory to the one-loop order.Comment: Prepared for the First Mediterranean Conference on Classical and Quantum Gravity, MCCQG, Kolymbari (Crete, Greece), 14-18 September, 2009; also, ERE2009: Gravitation in the Large, Bilbao (Spain), 7-11 September, 200

Systematic Review On Bullying Situations And Intervention Programs In Early Childhood Education

Background: Bullying is a traumatic event that is observed from the early stages of the educational system. Ensuring an adequate socioemotional development through the implementation of activities or intervention programs based on the work of Emotional Intelligence in the Early Childhood Education stage where empathy, assertiveness, emotional intelligence, social skills, values... are worked on, with the aim of preventing/eradicating situations of violence in educational environments, is essential to put an end to this problem. Goal: To find out whether there is evidence of bullying at the early childhood education (ECE) stage and to investigate whether bullying prevention work is carried out at this educational stage. Method: A systematic review, following the PRISMA 2020 Declaration, was carried out in the Web of Science (WoS) and Scopus (SJR) databases from January to March 2023. The search terms used were: "Children", "Health Education" and "Bullying". Only articles in Spanish and English published from 2013 to 2023 were selected, in which bullying in the ECE stage is analyzed. The last filter applied was related with research areas of Psychology and Education. The sample is composed of a total of 4 articles, most of them published abroad. Findings: According to the studies reviewed, there is little concern about bullying at the ECE stage, and there is little concern about the implementation of prevention programs for this proble

Modeling of the human alveolar rhabdomyosarcoma Pax3-Foxo1 chromosome translocation in mouse myoblasts using CRISPR-Cas9 nuclease

Many recurrent chromosome translocations in cancer result in the generation of fusion genes that are directly implicated in the tumorigenic process. Precise modeling of the effects of cancer fusion genes in mice has been inaccurate, as constructs of fusion genes often completely or partially lack the correct regulatory sequences. The reciprocal t(2;13)(q36.1;q14.1) in human alveolar rhabdomyosarcoma (A-RMS) creates a pathognomonic PAX3-FOXO1 fusion gene. In vivo mimicking of this translocation in mice is complicated by the fact that Pax3 and Foxo1 are in opposite orientation on their respective chromosomes, precluding formation of a functional Pax3-Foxo1 fusion via a simple translocation. To circumvent this problem, we irreversibly inverted the orientation of a 4.9 Mb syntenic fragment on chromosome 3, encompassing Foxo1, by using Cre-mediated recombination of two pairs of unrelated oppositely oriented LoxP sites situated at the borders of the syntenic region. We tested if spatial proximity of the Pax3 and Foxo1 loci in myoblasts of mice homozygous for the inversion facilitated Pax3-Foxo1 fusion gene formation upon induction of targeted CRISPR-Cas9 nuclease-induced DNA double strand breaks in Pax3 and Foxo1. Fluorescent in situ hybridization indicated that fore limb myoblasts show a higher frequency of Pax3/Foxo1 co-localization than hind limb myoblasts. Indeed, more fusion genes were generated in fore limb myoblasts via a reciprocal t(1;3), which expressed correctly spliced Pax3-Foxo1 mRNA encoding Pax3-Foxo1 fusion protein. We conclude that locus proximity facilitates chromosome translocation upon induction of DNA double strand breaks. Given that the Pax3-Foxo1 fusion gene will contain all the regulatory sequences necessary for precise regulation of its expression, we propose that CRISPR-Cas9 provides a novel means to faithfully model human diseases caused by chromosome translocation in mice.[Author Summary]: Many cancers carry recurrent chromosome translocations, which often result in the formation of fusion genes that are directly involved in the tumorigenic process. Alveolar rhabdomyosarcoma, a muscle tumor in children, is typified by a translocation that fuses the PAX3 gene on chromosome 2 to the FOXO1 gene on chromosome 13. For translocation to occur both genes need to break and the disparate ends need to fuse via a process called non-homologous end joining. We determined that physical proximity of Pax3 and Foxo1 in mouse muscle progenitor cells (myoblasts) facilitates fusion gene formation. Because Pax3 and Foxo1 in the mouse are in an opposite orientation, we used a chromosome engineering strategy to invert the orientation of Foxo1 so that upon translocation a productive Pax3-Foxo1 fusion gene is created. Co-localization of the Pax3 and Foxo1 loci is higher in fore limb than in hind limb myoblasts. Simultaneous induction of a targeted double strand DNA break in each gene by CRISPR-Cas9 nuclease generated more fusion genes in fore limb than in hind limb myoblasts. Thus, gene proximity facilitates fusion gene formation. We propose that CRISPR-Cas9 nuclease can be used for the precise modeling of chromosome translocations of human cancer in mice.This work was supported by the Van Vleet foundation of Memphis and ALSAC of St Jude Children’s Research Hospital and the Cancer Center Core Grant 5P30CA021765-34. BVB and JJC were supported by grant C1178/A4520 from Cancer Research UK.Peer Reviewe

Short-term energy recovery control for virtual inertia provision by renewable energy sources

The proliferation of Converter-Interfaced Renewable Energy Sources (CIRES), which are inertia-less, and the gradual decommissioning of synchronous generation have posed several challenges to the electric power system. This has motivated a complete a shift in the CIRES design and its corresponding control philosophy. Integrating Energy Storage Systems (ESS) within CIRES enables the implementation of different operating modes allowing them to provide ancillary services (AS) in a similar way to the synchronous generation. In order to tackle with those short-term response AS, such as virtual inertia, fast ESS (FESS) solutions with high power-to-energy ratio, particularly flywheels and supercapacitors, are preferred. In spite of several control algorithms have been proposed to provide such fast AS, very little research effort has been paid on the proper FESS energy recovery after the AS provision. This task is particularly challenging, since supercapacitors must be operated at a certain state of charge to guarantee that the required AS can be provided within its operational limits. This paper aims to fill this gap by proposing a new energy recovery control scheme for supercapacitors after the provision of short-term AS, such as virtual inertia. The proposed control is validated via simulations which clearly highlights its adequate performance.Horizonte 2020 (Unión Europea) 764090Ministerio de Economía y Competitividad (MINECO). España ENE2017-84813-R

Na+/K+-ATPase is a new interacting partner for the neuronal glycine transporter GlyT2 that downregulates its expression in vitro and in vivo

The neuronal glycine transporter GlyT2 plays a fundamental role in the glycinergic neurotransmission by recycling the neurotransmitter to the presynaptic terminal. GlyT2 is the main supplier of glycine for vesicle refilling, a process that is absolutely necessary to preserve quantal glycine content in synaptic vesicles. Alterations in GlyT2 activity modify glycinergic neurotransmission and may underlie several neuromuscular disorders, such as hyperekplexia, myoclonus, dystonia, and epilepsy. Indeed, mutations in the gene encoding GlyT2 are the main presynaptic cause of hyperekplexia in humans and produce congenital muscular dystonia type 2 (CMD2) in Belgian Blue cattle. GlyT2 function is strictly coupled to the sodium electrochemical gradient actively generated by the Na+/K+-ATPase (NKA). GlyT2 cotransports 3Na+/Cl-/glycine generating large rises of Na+ inside the presynaptic terminal that must be efficiently reduced by the NKA to preserve Na+ homeostasis. In this work, we have used high-throughput mass spectrometry to identify proteins interacting with GlyT2 in the CNS. NKA was detected as a putative candidate and through reciprocal coimmunoprecipitations and immunocytochemistry analyses the association between GlyT2 and NKA was confirmed. NKA mainly interacts with the raft-associated active pool of GlyT2, and low and high levels of the specific NKA ligand ouabain modulate the endocytosis and total expression of GlyT2 in neurons. The ouabain-mediated downregulation of GlyT2 also occurs in vivo in two different systems: zebrafish embryos and adult rats, indicating that this NKA-mediated regulatory mechanism is evolutionarily conserved and may play a relevant role in the physiological control of inhibitory glycinergic neurotransmission

Toxicity study in a pig model of intraperitoneal collagenase as an “enzymatic scalpel” directed to break stroma in order to generate a new perspective for peritoneal carcinomatosis approach: an experimental research

Background: This study aimed to measure the toxicity resulting from collagenase administration to the peritoneal cavity in a pig model as a preliminary step to break down the stroma surrounding tumors. Methods: Eight pigs were treated with 2 different collagenase concentrations previously tested in rats by our group. Time and temperature were controlled using a peritoneal lavage system (PRS System, Combat Medical Ltd.) identical to that used in human surgeries through hyperthermic intraperitoneal chemotherapy (HIPEC); 2 additional pigs were treated with peritoneal lavage only. Samples of blood and peritoneal fluid were collected pre-treatment, immediately after treatment, and 24 h postoperatively. In addition, histological studies and blood collagenase levels were measured. Results: No complications were observed during the surgeries. Intraoperative images evidenced the release of peritoneal tissue during collagenase treatment. After surgery, the animals showed no signs of pain. Diet and mobility were normal at 4 h postoperatively, and there were no significant differences in hematologic or biochemical parameters. Quantification of MMP1 and MMP2 in all samples as measured by absorbance showed no differences in blood collagenase levels between pre-treatment, post-treatment, and 24 h postoperatively. None of the animals treated with collagenase showed peritoneal adhesions during the second surgery. Histologically, peritoneal organs and serous structures did not show any microscopic alterations associated with collagenase treatment in any group. Conclusion: Lavage of the peritoneal cavity with doses of up to 100,000 collagen digestion units/animal for 30 min is safe and removes connective tissue from the peritoneal cavity

Mediating the Presence of Others: Reconceptualising Co-Presence as Mediated Intimacy

Drawing insight from queer and media studies, this article analyses data from the UK study Adults’ Media Lives. The authors claim that this study reveals the significance of people’s intimate relationships to their media practices, highlighting in particular how people’s media practices mediate the ‘presence’ of others. The authors put forward the concept of mediated intimacy to capture both the cultural intimacy people have with media and the mediation of intimacy by media practices. Mediating intimacy has implications for normative conceptions of intimate life, including the significance of ‘time’ to the values of ‘home’ and ‘work’

Full annual monitoring of Subantarctic Emiliania huxleyi populations reveals highly calcified morphotypes in high-CO2 winter conditions

Datos de investigación en: http://hdl.handle.net/10366/143074[EN]Ocean acidifcation is expected to have detrimental consequences for the most abundant calcifying phytoplankton species Emiliania huxleyi. However, this assumption is mainly based on laboratory manipulations that are unable to reproduce the complexity of natural ecosystems. Here, E. huxleyi coccolith assemblages collected over a year by an autonomous water sampler and sediment traps in the Subantarctic Zone were analysed. The combination of taxonomic and morphometric analyses together with in situ measurements of surface-water properties allowed us to monitor, with unprecedented detail, the seasonal cycle of E. huxleyi at two Subantarctic stations. E. huxleyi subantarctic assemblages were composed of a mixture of, at least, four diferent morphotypes. Heavier morphotypes exhibited their maximum relative abundances during winter, coinciding with peak annual TCO2 and nutrient concentrations, while lighter morphotypes dominated during summer, coinciding with lowest TCO2 and nutrients levels. The similar seasonality observed in both time-series suggests that it may be a circumpolar feature of the Subantarctic zone. Our results challenge the view that ocean acidifcation will necessarily lead to a replacement of heavily-calcifed coccolithophores by lightly-calcifed ones in subpolar ecosystems, and emphasize the need to consider the cumulative efect of multiple stressors on the probable succession of morphotypes.European Union's Horizon 2020, Marie Skłodowska-Curie Individual fellowshi

Full annual monitoring of Subantarctic Emiliania huxleyi populations reveals highly calcified morphotypes in high-CO2 winter conditions [Dataset]

[EN]Supplement Table S1. a. Sampling dates and morphotype relative abundance of E. huxleyi coccolith assemblages collected in the surface layer at the SOTS site. b. Sampling intervals, fluxes and morphotype relative abundance and morphometric measurements of E. huxleyi coccolith assemblages intercepted by the sediment traps at the SOTS and SAM sites. Table S2. Environmental parameters measured at the surface layer of the SOTS site from August 2011 to July 2012.European Union's Horizon 2020, Marie Skłodowska-Curie Individual fellowshipThe dataset includes Supplementary Information, Table S1. : abundance, composition and morphometric data of E. huxleyi coccolith assemblages generated during the current study Table S2: environmental data Environmental parameters measured at the surface layer of the SOTS site from August 2011 to July 2012