Elucidating how the saprophytic fungus Aspergillus nidulans uses the plant polyester suberin as carbon source

Lipid polymers in plant cell walls, such as cutin and suberin, build recalcitrant hydrophobic protective barriers. Their degradation is of foremost importance for both plant pathogenic and saprophytic fungi. Regardless of numerous reports on fungal degradation of emulsified fatty acids or cutin, and on fungi-plant interactions, the pathways involved in the degradation and utilisation of suberin remain largely overlooked. As a structural component of the plant cell wall, suberin isolation, in general, uses harsh depolymerisation methods that destroy its macromolecular structure. We recently overcame this limitation isolating suberin macromolecules in a near-native state.work partially supported by a grant from Iceland, Liechtenstein and Norway through the EEA financial mechanism (Project PT015), FCT: grant (PEst-OE/EQB/LA0004/2013) and fellowships (SFRH/BD/38378/2007, SFRH/BD/66396/2009, SFRH/BD/66030/2009, SFRH/BD/48286/2008), Fundação Calouste Gulbenkian fellowship (21-95587-B)

Clinical utility of F-18-FDG PET/CT in the follow-up of a large cohort of patients with high-risk differentiated thyroid carcinoma

Objective: To evaluate the clinical utility of F-18-FDG PET/CT in patients with high-risk DTC. Subjects and methods: Single-center retrospective study with 74 patients with high-risk differentiated thyroid cancer (DTC), classified in 4 groups. Group 1: patients with positive sTg or TgAb, subdivided in Group 1A: negative RxWBS and no foci of metastases identified at conventional image (n = 9)Group 1B: RxWBS not compatible with suspicious foci at conventional image or not proportional to sTg level (n = 13)Group 2: patients with histological findings of aggressive DTC variants (n = 21) and Group 3: patients with positive RxWBS (n = 31). Results: F-18-FDG PET/CT identified undifferentiated lesions and helped restage the disease in groups 1B and 2. The scan helped guide clinical judgment in 9/13 (69%) patients of group 1B, 10/21 (48%) patients of group 2 and 2/31 (6%) patients of group 3. There was no clinical benefit associated with group 1A. F-18-FDG PET/CT was associated with progressive disease. Conclusion: F-18-FDG PET/CT is a useful tool in the follow-up of patients with high-risk DTC, mainly in the group of RxWBS not compatible with suspicious foci at conventional image or not proportional to sTg level and in those with aggressive DTC variants. Additionally, this study showed that F-18-FDG PET/CT was associated with progression and helped display undifferentiated lesions guiding clinical assessments regarding surgeries or expectant treatments.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Ministério da Saúde (MS)Univ Fed São Paulo EPM Unifesp, Escola Paulista Med, Ctr Doencas Tireoide, São Paulo, SP, BrazilUniv Fed São Paulo EPM Unifesp, Escola Paulista Med, Lab Endocrinol Mol & Translac, Dept Med,Div Endocrinol, São Paulo, SP, BrazilHIAE, Dept Imagem, São Paulo, SP, BrazilUniv Fed São Paulo EPM Unifesp, Escola Paulista Med, Ctr Doencas Tireoide, São Paulo, SP, BrazilUniv Fed São Paulo EPM Unifesp, Escola Paulista Med, Lab Endocrinol Mol & Translac, Dept Med,Div Endocrinol, São Paulo, SP, BrazilFAPESP: 2006/60402-1MS: 25000.168513/2008-11Web of Scienc

Observation of Interlayer Phonon Modes in van der Waals Heterostructures

We have investigated the vibrational properties of van der Waals heterostructures of monolayer transition metal dichalcogenides (TMDs), specifically MoS2/WSe2 and MoSe2/MoS2 heterobilayers and twisted MoS2 bilayers, by means of ultralow-frequency Raman spectroscopy. We discovered Raman features (at 30–40 cm−1) that arise from the layer-breathing mode (LBM) vibration between the two incommensurate TMD monolayers in these structures. The LBM Raman intensity correlates strongly with the suppression of photoluminescence that arises from interlayer charge transfer. The LBM is generated only in bilayer areas with direct layer-layer contact and an atomically clean interface. Its frequency also evolves systematically with the relative orientation between the two layers. Our research demonstrates that the LBM can serve as a sensitive probe to the interface environment and interlayer interactions in van der Waals materials

Beyond spectroscopy. II. Stellar parameters for over twenty million stars in the northern sky from SAGES DR1 and Gaia DR3

We present precise photometric estimates of stellar parameters, including effective temperature, metallicity, luminosity classification, distance, and stellar age, for nearly 26 million stars using the methodology developed in the first paper of this series, based on the stellar colors from the Stellar Abundances and Galactic Evolution Survey (SAGES) DR1 and Gaia EDR3. The optimal design of stellar-parameter sensitive $uv$ filters by SAGES has enabled us to determine photometric-metallicity estimates down to $-3.5$, similar to our previous results with the SkyMapper Southern Survey (SMSS), yielding a large sample of over five million metal-poor (MP; [Fe/H]$\le -1.0$) stars and nearly one million very metal-poor (VMP; [Fe/H]$\le -2.0$) stars. The typical precision is around $0.1$ dex for both dwarf and giant stars with [Fe/H]$>-1.0$, and 0.15-0.25/0.3-0.4 dex for dwarf/giant stars with [Fe/H]$<-1.0$. Using the precise parallax measurements and stellar colors from Gaia, effective temperature, luminosity classification, distance and stellar age are further derived for our sample stars. This huge data set in the Northern sky from SAGES, together with similar data in the Southern sky from SMSS, will greatly advance our understanding of the Milky Way, in particular its formation and evolution.Comment: 14 pages, 14 figures, 3 tables, accepted by ApJ. arXiv admin note: text overlap with arXiv:2104.1415

Macrocalcitonin Is a Novel Pitfall in the Routine of Serum Calcitonin Immunoassay

Context: Calcitonin (CT) is a sensitive marker of medullary thyroid carcinoma (MTC) and is used for primary diagnosis and follow-up after thyroidectomy. However, persistently elevated CT is observed even after complete surgical removal without evidence of a recurrent or persistent tumor. Objective: To investigate the presence of assay interference in the serum CT of MTC patients who are apparently without a structural disease. Patients and Methods: We studied three index MTC cases for CT assay interference and 14 patients with metastatic MTC. The CT level was measured using an immunofluorometric assay. Screening for assay interference was performed by determination of CT levels before and after serum treatment with polyethylene glycol. Additionally, samples were analyzed by chromatography on ultra-performance liquid chromatography and protein A-Sepharose. Results: Patients with biochemical and structural disease showed CT mean recovery of 84.1% after polyethylene glycol treatment, whereas patients suspected of interference showed recovery from 2-7%. The elution profile on UPLC showed that the immunometric CT from these three patients behaved like a high molecular mass aggregate (>300 kDa). Additionally, when these samples were applied to the protein A-Sepharose, CT immunoreactivity was retained on the column and was only released after lowering the pH. Conclusions: For the first time, our results show the presence of a novel pitfall in the CT immunoassay: "macrocalcitonin." Its etiology, frequency, and meaning remain to be defined, but its recognition is of interest and can help clinicians avoid unnecessary diagnostic investigations and treatment during the follow-up of MTC.Sao Paulo State Research Foundation-FAPESPFAPESPFederal Agency of Support and Evaluation of Postgraduate Education (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)National Council for Scientific and Technological DevelopmentUniv Fed Sao Paulo, Escola Paulista Med, Div Endocrinol, Dept Med,Thyroid Dis Ctr, BR-04039032 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Div Endocrinol, Dept Med,Lab Mol & Translat Endocrinol, BR-04039032 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Biochem, Div Mol Biol, BR-04044020 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Div Endocrinol, Dept Med,Thyroid Dis Ctr, BR-04039032 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Div Endocrinol, Dept Med,Lab Mol & Translat Endocrinol, BR-04039032 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Biochem, Div Mol Biol, BR-04044020 Sao Paulo, SP, BrazilFAPESP: 2006/60402-1FAPESP: 2010/51547-1FAPESP: 2010/19478Web of Scienc

The Low-redshift Lyman Continuum Survey: Radio continuum properties of low-zz Lyman continuum emitters

The sources that leak Lyman-continuum (LyC) photons and lead to the reionisation of the universe are intensely studied using multiple observing facilities. Recently, the Low-redshift LyC Survey (LzLCS) has found the first large sample of LyC emitting galaxies at low redshift ($z\sim 0.3$) with the Hubble Space Telescope/Cosmic Origins Spectrograph. The LzLCS sample contains a robust estimate of the LyC escape fraction ($f_\mathrm{esc}^\mathrm{LyC}$) for 66 galaxies spanning a wide range of $f_\mathrm{esc}^\mathrm{LyC}$. Here we, for the first time, aim to study the radio continuum (RC) properties of LzLCS sources and their dependence on $f_\mathrm{esc}^\mathrm{LyC}$. We present Karl G. Jansky Very Large Array RC observations at C (4-8 GHz), S (2-4 GHz) and L (1-2 GHz) bands for a sub-sample of the LzLCS sources. The radio spectral index ($\alpha^{\mathrm{3GHz}}_\mathrm{6GHz}$) spans a wide range from being flat ( $\geq -0.1$) to very steep ($\leq -1.0$). We find that the strongest leakers in our sample show flat $\alpha^{\mathrm{3GHz}}_\mathrm{6GHz}$, weak leakers have $\alpha^{\mathrm{3GHz}}_\mathrm{6GHz}$ close to normal star-forming galaxies, and non-leakers are characterized by steep $\alpha^{\mathrm{3GHz}}_\mathrm{6GHz}$. We argue that a combination of young ages, free-free absorption, and a flat cosmic-ray energy spectrum can altogether lead to a flat $\alpha^{\mathrm{3GHz}}_\mathrm{6GHz}$ for strong leakers. Non-leakers are characterized by steep spectra which can arise due to break/cutoff at high frequencies. Such a cutoff in the spectrum can arise in a single injection model of CRs characteristic of galaxies which have recently stopped star formation. Such a relation between $\alpha^{\mathrm{3GHz}}_\mathrm{6GHz}$ and $f_\mathrm{esc}^\mathrm{LyC}$ hints at the interesting role of supernovae, CRs, and magnetic fields in facilitating the escape (and/or the lack) of LyC photons.Comment: 25 pages, 14 figures, 3 tables, Submitted to Astronomy & Astrophysic

Selective deletion of endothelial cell calpain in mice reduces diabetic cardiomyopathy by improving angiogenesis

Aims/hypothesis: The role of non-cardiomyocytes in diabetic cardiomyopathy has not been fully addressed. This study investigated whether endothelial cell calpain plays a role in myocardial endothelial injury and microvascular rarefaction in diabetes, thereby contributing to diabetic cardiomyopathy. Methods: Endothelial cell-specific Capns1-knockout (KO) mice were generated. Conditions mimicking prediabetes and type 1 and type 2 diabetes were induced in these KO mice and their wild-type littermates. Myocardial function and coronary flow reserve were assessed by echocardiography. Histological analyses were performed to determine capillary density, cardiomyocyte size and fibrosis in the heart. Isolated aortas were assayed for neovascularisation. Cultured cardiac microvascular endothelial cells were stimulated with high palmitate. Angiogenesis and apoptosis were analysed. Results: Endothelial cell-specific deletion of Capns1 disrupted calpain 1 and calpain 2 in endothelial cells, reduced cardiac fibrosis and hypertrophy, and alleviated myocardial dysfunction in mouse models of diabetes without significantly affecting systemic metabolic variables. These protective effects of calpain disruption in endothelial cells were associated with an increase in myocardial capillary density (wild-type vs Capns1-KO 3646.14 ± 423.51 vs 4708.7 ± 417.93 capillary number/high-power field in prediabetes, 2999.36 ± 854.77 vs 4579.22 ± 672.56 capillary number/high-power field in type 2 diabetes and 2364.87 ± 249.57 vs 3014.63 ± 215.46 capillary number/high-power field in type 1 diabetes) and coronary flow reserve. Ex vivo analysis of neovascularisation revealed more endothelial cell sprouts from aortic rings of prediabetic and diabetic Capns1-KO mice compared with their wild-type littermates. In cultured cardiac microvascular endothelial cells, inhibition of calpain improved angiogenesis and prevented apoptosis under metabolic stress. Mechanistically, deletion of Capns1 elevated the protein levels of β-catenin in endothelial cells of Capns1-KO mice and constitutive activity of calpain 2 suppressed β-catenin protein expression in cultured endothelial cells. Upregulation of β-catenin promoted angiogenesis and inhibited apoptosis whereas knockdown of β-catenin offset the protective effects of calpain inhibition in endothelial cells under metabolic stress. Conclusions/interpretation: These results delineate a primary role of calpain in inducing cardiac endothelial cell injury and impairing neovascularisation via suppression of β-catenin, thereby promoting diabetic cardiomyopathy, and indicate that calpain is a promising therapeutic target to prevent diabetic cardiac complications

Decreasing the use of edible oils in China using WeChat and theories of behavior change: study protocol for a randomized controlled trial.

The consumption of edible oils in China has increased rapidly in recent years, and the total amount of edible-oil intake in the country has ranked first in the world. The choice and intake of edible oils, as a source of fats, are important factors that affect people's health. Many chronic diseases are closely associated with high-calorie and saturated-fat intake. The influence of traditional concepts that promote the use of edible oils among women, particularly housewives, plays a key role in a household's diet and nutrition because the diet-related knowledge, attitude and behaviour of housewives are dominant factors in planning and preparing their family's meals. WeChat, which was developed by Tencent, is a multipurpose messaging, social media and mobile payment application (app) in China. Described by Forbes as one of the world's most powerful apps, WeChat provides considerable convenience in disseminating knowledge. Accordingly, this study aims to design a pilot intervention to decrease the use of edible oils in Chinese households. The intervention, which is based on theories of behaviour change, will be implemented through WeChat. The study design is a randomised controlled trial that adopts knowledge, attitude and practice, social cognitive and stages of change theories as theoretical models. A total of 800 housewives between the ages of 25 and 45 years will be recruited on WeChat and from the communities in four areas (including rural and urban) in Chongqing, China. A self-administered questionnaire will be used to collect information regarding age, educational level, occupation, family members, edible-oil intake habits, knowledge of edible oils and WeChat usage habits. A total of 200 participants will be selected and randomly assigned to two equal-sized groups: group A (the intervention group) and group B (the control group). Group A will receive health education regarding edible oils for four consecutive weeks, whereas group B will be treated as the blank control. Each participant will complete a battery of knowledge, attitude and behaviour tests immediately, 3 months and 6 months after the intervention. In addition, weight, moisture rate, fat rate, visceral fat level and body mass index will be calculated using a multifunctional weighing scale, namely, Tanita BC-601 (Japan). The study is currently in the design stage. This study aims to increase knowledge and awareness of the appropriate use of edible oils, thereby encouraging participants to change behaviour by decreasing the intake of unhealthy levels of edible oils. It will be the first intervention to investigate the use of edible oils in China through WeChat. We predict that receiving health education regarding edible oils through WeChat will substantially improve the knowledge and attitude of the respondents. The members of the intervention group will have increased awareness and will be willing to decrease their use of edible oils to remain healthy. Results of this study may provide scientific evidence for the effect of health education through WeChat on edible oil-intake behaviour, thereby offering a comprehensive intervention to decrease the use of edible oils and promote a healthy lifestyle. Chinese Clinical Trial Registry (primary registry in the World Health Organisation registry network): ChiCTR-IOR-17013472 . Registered on 21 November 2017