Allocation of Carbon-14 to Roots of Different Ages in Perennial Ryegrass (Lolium perenne L.)

The objective of this study was to provide information on how current photosynthate allocated to the root system in perennial ryegrass (Lolium perenne L.) is distributed among individual roots at differing nodal positions. Mature single ryegrass tillers were transplanted in September 1993 to glass house pots and three months later four of the plants were supplied with 14CO2. Individual roots from these radioactively-labeled plants were identified according to their nodal position on the tiller axis and amount of radiocarbon in each root quantified for root tip segments and for the remainder of the root axis. Similar plants were destructively harvested and the number of roots, and mean weight and mean length of individual roots at each node recorded. Counting basipetally from the node of the first fully expanded leaf, root initiation occurred by node two, and final root mass and length was attained by node six. Of the total radiocarbon recovered from the root system, approximately 70% was found in roots attached to the first five nodes. The greatest allocation to root tips was at node one (17.7% of radiocarbon recovered) while the greatest allocation to root axes was at node three (12.1% of radiocarbon recovered). Implications for understanding of timing of root development in relation to nodal position on the tiller axis are discussed

Calculating the entropy loss on adsorption of organic molecules at insulating surfaces

Although it is recognized that the dynamic behavior of adsorbing molecules strongly affects the entropic contribution to adsorption free energy, detailed studies of the adsorption entropy of large organic molecules at insulating surfaces are still rare. We compared adsorption of two different functionalized organic molecules, 1,3,5-tri(4-cyano-4,4-biphenyl)benzene (TCB) and 1,4-bis(cyanophenyl)-2,5-bis(decyloxy)benzene (CDB), on the KCl(001) surface using density functional theory (DFT) and molecular dynamics (MD) simulations. The accuracy of the van der Waals corrected DFT-D3 was benchmarked using Møller–Plesset perturbation theory calculations. Classical force fields were then parametrized for both the TCB and CDB molecules on the KCl(001) surface. These force fields were used to perform potential of mean force (PMF) calculations of adsorption of individual molecules and extract information on the entropic contributions to adsorption energy. The results demonstrate that entropy loss upon adsorption are significant for flexible molecules. Even at relatively low temperatures (e.g., 400 K), these effects can match the enthalpic contribution to adsorption energ

Coxsackievirus B3 Inhibits Antigen Presentation In Vivo, Exerting a Profound and Selective Effect on the MHC Class I Pathway

Many viruses encode proteins whose major function is to evade or disable the host T cell response. Nevertheless, most viruses are readily detected by host T cells, and induce relatively strong T cell responses. Herein, we employ transgenic CD4+ and CD8+ T cells as sensors to evaluate in vitro and in vivo antigen presentation by coxsackievirus B3 (CVB3), and we show that this virus almost completely inhibits antigen presentation via the MHC class I pathway, thereby evading CD8+ T cell immunity. In contrast, the presentation of CVB3-encoded MHC class II epitopes is relatively unencumbered, and CVB3 induces in vivo CD4+ T cell responses that are, by several criteria, phenotypically normal. The cells display an effector phenotype and mature into multi-functional CVB3-specific memory CD4+ T cells that expand dramatically following challenge infection and rapidly differentiate into secondary effector cells capable of secreting multiple cytokines. Our findings have implications for the efficiency of antigen cross-presentation during coxsackievirus infection

The spectrum of mutations and molecular pathogenesis of hemophilia A in 181 Portuguese patients

Disease-causing alterations within the F8 gene were identified in 177 hemophilia A families of Portuguese origin. The spectrum of non-inversion F8 mutations in 101 families included 67 different alterations, namely: 36 missense, 8 nonsense and 4 splice site mutations, as well as 19 insertions/deletions. Thirty-four of these mutations are novel. Molecular modeling allowed prediction of the conformational changes introduced by selected amino acid substitutions and their correlation with the patients' phenotypes. The relatively frequent, population-specific, missense mutations together with de novo alterations can lead to significant differences in the spectrum of F8 mutations among different populationsThis study was partially supported by Fundação para a Ciência e a Tecnologia: research grant PBIC/C/SAU/1588/92 and Programa de Financiamento Plurianual do CIGM

Generic Tracking of Multiple Apparent Horizons with Level Flow

We report the development of the first apparent horizon locator capable of finding multiple apparent horizons in a ``generic'' numerical black hole spacetime. We use a level-flow method which, starting from a single arbitrary initial trial surface, can undergo topology changes as it flows towards disjoint apparent horizons if they are present. The level flow method has two advantages: 1) The solution is independent of changes in the initial guess and 2) The solution can have multiple components. We illustrate our method of locating apparent horizons by tracking horizon components in a short Kerr-Schild binary black hole grazing collision.Comment: 13 pages including figures, submitted to Phys Rev

Dust Formation Above Cool Magnetic Spots in Evolved Stars

We examine the structure of cool magnetic spots in the photospheres of evolved stars, specifically asymptotic giant branch (AGB) stars and R Coronae Borealis stars. We find that the photosphere of a cool magnetic spot will be above the surrounding photosphere of AGB stars, opposite to the situation in the sun. This results from the behavior of the opacity, which increases with decreasing temperature, opposite to the behavior of the opacity near the effective temperature of the sun. We analyze the formation of dust above the cool magnetic spots, and suggest that the dust formation is facilitated by strong shocks, driven by stellar pulsations, which run through and around the spots. The presence of both the magnetic field and cooler temperatures make dust formation easier as the shock passes above the spot. We review some observations supporting the proposed mechanism, and suggest further observations to check the model.Comment: 22 pages, uses aasms4.sty, preprin

Continuous recruitment of naive T cells contributes to heterogeneity of antiviral CD8 T cells during persistent infection

Numerous microbes establish persistent infections, accompanied by antigen-specific CD8 T cell activation. Pathogen-specific T cells in chronically infected hosts are often phenotypically and functionally variable, as well as distinct from T cells responding to nonpersistent infections; this phenotypic heterogeneity has been attributed to an ongoing reencounter with antigen. Paradoxically, maintenance of memory CD8 T cells to acutely resolved infections is antigen independent, whereas there is a dependence on antigen for T cell survival in chronically infected hosts. Using two chronic viral infections, we demonstrate that new naive antigen-specific CD8 T cells are primed after the acute phase of infection. These newly recruited T cells are phenotypically distinct from those primed earlier. Long-lived antiviral CD8 T cells are defective in self-renewal, and lack of thymic output results in the decline of virus-specific CD8 T cells, indicating that newly generated T cells preserve antiviral CD8 T cell populations during chronic infection. These findings reveal a novel role for antigen in maintaining virus-specific CD8 T cells during persistent infection and provide insight toward understanding T cell differentiation in chronic infection

Dynamically Driven Evolution of the Interstellar Medium in M51

Massive star formation occurs in giant molecular clouds (GMCs); an understanding of the evolution of GMCs is a prerequisite to develop theories of star formation and galaxy evolution. We report the highest-fidelity observations of the grand-design spiral galaxy M51 in carbon monoxide (CO) emission, revealing the evolution of GMCs vis-a-vis the large-scale galactic structure and dynamics. The most massive GMCs (giant molecular associations (GMAs)) are first assembled and then broken up as the gas flow through the spiral arms. The GMAs and their H_2 molecules are not fully dissociated into atomic gas as predicted in stellar feedback scenarios, but are fragmented into smaller GMCs upon leaving the spiral arms. The remnants of GMAs are detected as the chains of GMCs that emerge from the spiral arms into interarm regions. The kinematic shear within the spiral arms is sufficient to unbind the GMAs against self-gravity. We conclude that the evolution of GMCs is driven by large-scale galactic dynamics—their coagulation into GMAs is due to spiral arm streaming motions upon entering the arms, followed by fragmentation due to shear as they leave the arms on the downstream side. In M51, the majority of the gas remains molecular from arm entry through the interarm region and into the next spiral arm passage

First results from CARMA: the combined array for research in millimeter-wave astronomy

The Combined Array for Research in Millimeter-wave Astronomy (CARMA) comprises the millimeter-wave antennas of the Owens Valley Radio Observatory (OVRO), the Berkeley-Illinois-Maryland Association (BIMA) Array, and the new Sunyaev-Zel'dovich Array (SZA). CARMA consists of six 10.4-m, nine 6.1-m, and eventually eight 3.5-m diameter antennas on a site at elevation 2200 m in the Inyo Mountains near Bishop, California. The array will be operated by an association that includes the California Institute of Technology and the Universities of California (Berkeley), Chicago, Illinois (Urbana-Champaign), and Maryland. Observations will be supported at wavelengths of 1 cm, 3 mm, and 1.3 mm, on baselines from 5 m to 2 km. The initial correlator will use field programmable gate array (FPGA) technology to provide all single-polarization cross-correlations on two subarrays of 8 and 15 antennas with a total bandwidth of 8 GHz on the sky. The next generation correlator will correlate the full 23-antenna array in both polarizations. CARMA will support student training, technology development, and front-line astronomical research in a wide range of fields including cosmology, galaxy formation and evolution, star and planet formation, stellar evolution, chemistry of the interstellar medium, and within the Solar System, comets, planets, and the Sun. Commissioning of CARMA began in August 2005, after relocation of the antennas to the new site. The first science observations commenced in April 2006