873 research outputs found
Calculations of energy levels and lifetimes of low-lying states of barium and radium
We use the configuration interaction method and many-body perturbation theory
to perform accurate calculations of energy levels, transition amplitudes, and
lifetimes of low-lying states of barium and radium. Calculations for radium are
needed for the planning of measurements of parity and time invariance violating
effects which are strongly enhanced in this atom. Calculations for barium are
used to control the accuracy of the calculations.Comment: 8 page
Super-resolution imaging and estimation of protein copy numbers at single synapses with DNA-PAINT
In the brain, the strength of each individual synapse is defined by the complement of proteins present or the "local proteome." Activity-dependent changes in synaptic strength are the result of changes in this local proteome and posttranslational protein modifications. Although most synaptic proteins have been identified, we still know little about protein copy numbers in individual synapses and variations between synapses. We use DNA-point accumulation for imaging in nanoscale topography as a single-molecule super-resolution imaging technique to visualize and quantify protein copy numbers in single synapses. The imaging technique provides near-molecular spatial resolution, is unaffected by photobleaching, enables imaging of large field of views, and provides quantitative molecular information. We demonstrate these benefits by accessing copy numbers of surface AMPA-type receptors at single synapses of rat hippocampal neurons along dendritic segments
Traveling-wave deceleration of SrF molecules
We report on the production, deceleration and detection of a SrF molecular
beam. The molecules are captured from a supersonic expansion and are
decelerated in the X state. We demonstrate the removal
of up to 40% of the kinetic energy with a 2 meter long modular traveling-wave
decelerator. Our results demonstrate a crucial step towards the preparation of
ultracold gases of heavy diatomic molecules for precision spectroscopy
Single-molecule super-resolution imaging of chromosomes and in situ haplotype visualization using Oligopaint FISH probes
Fluorescence in situ hybridization (FISH) is a powerful single-cell technique for studying nuclear structure and organization. Here we report two advances in FISH-based imaging. We first describe the in situ visualization of single-copy regions of the genome using two single-molecule super-resolution methodologies. We then introduce a robust and reliable system that harnesses single-nucleotide polymorphisms (SNPs) to visually distinguish the maternal and paternal homologous chromosomes in mammalian and insect systems. Both of these new technologies are enabled by renewable, bioinformatically designed, oligonucleotide-based Oligopaint probes, which we augment with a strategy that uses secondary oligonucleotides (oligos) to produce and enhance fluorescent signals. These advances should substantially expand the capability to query parent-of-origin-specific chromosome positioning and gene expression on a cell-by-cell basis
Europa. Central. 1:1850000
Coordenadas : E35 05'-E43 00'/N49 40'-N44 20'. Meridiano de Ferro. Constan ademas coordenadas segun el meridiano de ParÃsRelieve : sombreadoLeyenda de signos utilizadosInserta : Pest - Ofen. - Escala 1:5000
Quantitative single-protein imaging reveals molecular complex formation of integrin, talin, and kindlin during cell adhesion
Single-molecule localization microscopy (SMLM) enabling the investigation of individual proteins on molecular scales has revolutionized how biological processes are analysed in cells. However, a major limitation of imaging techniques reaching single-protein resolution is the incomplete and often unknown labeling and detection efficiency of the utilized molecular probes. As a result, fundamental processes such as complex formation of distinct molecular species cannot be reliably quantified. Here, we establish a super-resolution microscopy framework, called quantitative single-molecule colocalization analysis (qSMCL), which permits the identification of absolute molecular quantities and thus the investigation of molecular-scale processes inside cells. The method combines multiplexed single-protein resolution imaging, automated cluster detection, in silico data simulation procedures, and widely applicable experimental controls to determine absolute fractions and spatial coordinates of interacting species on a true molecular level, even in highly crowded subcellular structures. The first application of this framework allowed the identification of a long-sought ternary adhesion complex-consisting of talin, kindlin and active beta 1-integrin-that specifically forms in cell-matrix adhesion sites. Together, the experiments demonstrate that qSMCL allows an absolute quantification of multiplexed SMLM data and thus should be useful for investigating molecular mechanisms underlying numerous processes in cells. Single-molecule localisation microscopy is limited by low labeling and detection efficiencies of the molecular probes. Here the authors report a framework to obtain absolute molecular quantities on a true molecular scale; the data reveal a ternary adhesion complex underlying cell-matrix adhesion
A biotecnologia nos programas de melhoramento de forrageiras tropicais da Embrapa Gado de Corte.
Neste trabalho procurou-se apresentar e discutir, de forma ampla, o uso da biotecnologia e seu potencial para os programas de melhoramento de forrageiras tropicais realizados na Embrapa Gado de Corte. O uso da biotecnologia nesses programas é uma atividade recente, mas importantes resultados vêm sendo gerados a fim de auxiliar o processo de obtenção de novas cultivares forrageiras. A maioria dos trabalhos apresentados utiliza marcadores Random Amplification of Polymorphic DNA (RAPD) para aplicações em curto prazo: estudos de diversidade em acessos de bancos de germoplasma, identificação de hÃbridos e estimação da taxa de cruzamento. Aplicações em médio e longo prazos do uso de marcadores, como mapeamento genético e seleção auxiliada por marcadores moleculares (SAMM), ainda necessitam de maiores investimentos, tanto na busca de novos marcadores, quanto no desenvolvimento de populações adequadas para esses estudos. Em 2007, teve inÃcio uma nova linha de pesquisa nessa unidade, a prospecção de genes com caracterÃsticas econômicas. Genes para a tolerância ao alumÃnio são o foco dessa pesquisa que pretende explorar a sintenia entre os genomas de gramÃneas, visando ao desenvolvimento de cultivares de braquiária mais tolerantes ao alumÃnio. A Embrapa Gado de Corte vem investindo em pessoal e aquisição de equipamentos para avançar não só na produção de cultivares de forrageiras mais adaptadas à s necessidades de um mercado cada vez mais exigente, como também no crescimento institucional do setor de biotecnologia.bitstream/CNPGC-2009-09/12403/1/DOC168.pd
Investigations of Ra properties to test possibilities of new optical frequency standards
The present work tests the suitability of the narrow transitions $7s \
^2S_{1/2} \to 6d ^2D_{3/2}7s ^2S_{1/2} \to 6d ^2D_{5/2}^+6d^+$ to be considered as a potential
candidate for an atomic clock. This is further corroborated by our studies of
the hyperfine interactions, dipole and quadrupole polarizabilities and
quadrupole moments of the appropriate states of this system.Comment: Latex files, 5 pages, 1 figur
Europa. Central. 1:925000
Coordenadas : E27 30-E32 37'/52 02'-N49 58'. Meridiano de Ferro. Constan ademas coordenadas segun el meridiano de ParÃsRelieve : sombreadoLeyenda de signos utilizadosInserta : un mapa de altitude
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