MicroRNA-433 Dampens Glucocorticoid Receptor Signaling, Impacting Circadian Rhythm and Osteoblastic Gene Expression

FUNDING This work was supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health [AR44877]; the National Institutes for Dental and Craniofacial Research [5T90DE21989]; a Grant-in-Aid award from the American Society for Bone and Mineral Research; the UConn Health Center Research Advisory council; and the Center for Molecular Medicine at UConn Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.Peer reviewedPublisher PD

Synthesis of Polysaccharide Macrobolaforms

International audienceNeutral polysaccharides are very abundant renewable natural products that have been identified as alternative substrates for use in the field of polymer commmodities. Unfortunately beside other obstacles such as hydrophilicity, polysaccharides can display very low shape factors (molecular length/width) as compared to common synthetic polymers. This shape factor is often unsuited for creating the molecular entanglements, necessary for material physical cohesion for applications in the field of plastic material. In order to overcome this drawback various strategies have been applied to extend the length of these macromolecules, from controlled chemical cross-linking to macromolecular fractionation. Due to the very large number of chemically similar hydroxyl functions present on the macromolecular polymer backbone, selective chemical intermolecular-linking of hydroxyl groups is almost impossible to control, and very rapidly leads to unsuited tri-dimensional networks. Taking advantage of the unique chemical nature of one of the extremities (the reducing end) we developed several techniques of selective dimerisation of oligo-and polysaccharide, using diamine and dicarboxylic linkers. These dimerisations resulted in saccharidic bolaforms with improved shape factor by doubling of the molecular length. In the case of large polysaccharides the obtained macrobolaforms displayed interesting improved mechanical properties. Preliminary investigations of the structure/property relationships of these macrobolaforms show an improvement of mechanical behaviour, which was linked to the shape factor improvement and also to the ability to form oriented structures during material extrusion + dimerisation of polysaccharide

Field theoretic calculation of the surface tension for a model electrolyte system

We carry out the calculation of the surface tension for a model electrolyte to first order in a cumulant expansion about a free field theory equivalent to the Debye-H\"uckel approximation. In contrast with previous calculations, the surface tension is calculated directly without recourse to integrating thermodynamic relations. The system considered is a monovalent electrolyte with a region at the interface, of width h, from which the ionic species are excluded. In the case where the external dielectric constant epsilon_0 is smaller than the electrolyte solution's dielectric constant epsilon we show that the calculation at this order can be fully regularized. In the case where h is taken to be zero the Onsager-Samaras limiting law for the excess surface tension of dilute electrolyte solutions is recovered, with corrections coming from a non-zero value of epsilon_0/epsilon.Comment: LaTeX, 14 pages, 3 figures, 1 tabl

Dissecting the Cosmic Infrared Background with 3D Instruments

The cosmic infrared background (CIB) consists of emission from distant, dusty, star-forming galaxies. Energetically, the CIB is very important as it contains as much energy as the extragalactic optical background. The nature and evolutionary status of the objects making up the background are, however, unclear. The CIB peaks at ~150 microns, and as such is most effectively studied from space. The limited apertures of space-borne telescopes set the angular resolution that can be attained, and so even Herschel, with its 3.5m diameter, will be confusion-limited at this wavelengths at ~5mJy. The bulk of the galaxies contributing to the CIB are fainter than this, so it is difficult to study them without interferometry. Here we present the results of a preliminary study of an alternative way of probing fainter than the continuum confusion limit using far-IR imaging spectroscopy. An instrument capable of such observations is being planned for SPICA - a proposed Japanese mission with an aperture equivalent to that of Herschel and more than 2 orders of magnitude more sensitive. We investigate the potential of imaging spectrometers to break the continuum confusion limit. We have simulated the capabilities of a spectrometer with modest field of view (2'x2'), moderate spectral resolution (R~1-2000) and high sensitivity. We find that such an instrument is capable of not only detecting line emission from sources with continuum fluxes substantially below the confusion limit, but also of determining their redshifts and, where multiple lines are detected, some emission line diagnostics. We conclude that 3-D imaging spectrometers on cooled far-IR space telescopes will be powerful new tools for extragalactic far-IR astronomy.Comment: Accepted for publication in Astronomy & Astrophysic

Toward a script theory of guidance in computer-supported collaborative learning

This article presents an outline of a script theory of guidance for computer-supported collaborative learning (CSCL). With its four types of components of internal and external scripts (play, scene, role, and scriptlet) and seven principles, this theory addresses the question how CSCL practices are shaped by dynamically re-configured internal collaboration scripts of the participating learners. Furthermore, it explains how internal collaboration scripts develop through participation in CSCL practices. It emphasizes the importance of active application of subject matter knowledge in CSCL practices, and it prioritizes transactive over non-transactive forms of knowledge application in order to facilitate learning. Further, the theory explains how external collaboration scripts modify CSCL practices and how they influence the development of internal collaboration scripts. The principles specify an optimal scaffolding level for external collaboration scripts and allow for the formulation of hypotheses about the fading of external collaboration scripts. Finally, the article points towards conceptual challenges and future research questions

Linking stellar mass and star formation in Spitzer/MIPS 24 micron galaxies

We present deep Ks<21.5 (Vega) identifications, redshifts and stellar masses for most of the sources composing the bulk of the 24 micron background in the GOODS/CDFS. Our identified sample consists of 747 Spitzer/MIPS 24 micron objects, and includes ~94% of all the 24 micron sources in the GOODS-South field which have fluxes Snu(24)>83 microJy (the 80% completeness limit of the Spitzer/GTO 24 micron catalog). 36% of our galaxies have spectroscopic redshifts (mostly at z<1.5) and the remaining ones have photometric redshifts of very good quality, with a median of |dz|=|zspec-zphot|/(1+zspec)=0.02. We find that MIPS 24 micron galaxies span the redshift range z~0-4, and that a substantial fraction (28%) lie at high redshifts z>1.5. We determine the existence of a bump in the redshift distribution at z~1.9, indicating the presence of a significant population of galaxies with PAH emission at these redshifts. Massive (M>10^11 Msun) star-forming galaxies at redshifts 2<z<3 are characterized by very high star-formation rates (SFR>500 Msun/yr), and some of them are able to construct a mass of 10^10-10^11 Msun in a single burst lifetime (~0.01-0.1 Gyr). At lower redshifts z<2, massive star-forming galaxies are also present, but appear to be building their stars on long timescales, either quiescently or in multiple modest burst-like episodes. At redshifts z~1-2, the ability of the burst-like mode to produce entire galaxies in a single event is limited to some lower (M<7x10^10 Msun) mass systems, and it is basically negligible at z<1. Our results support a scenario where star-formation activity is differential with assembled stellar mass and redshift, and where the relative importance of the burst-like mode proceeds in a down-sizing way from high to low redshifts. (abridged)Comment: Accepted for publication in the ApJ. 19 pages, 10 figures. Uses emulateap

Violent crime exposure classification and adverse birth outcomes: a geographically-defined cohort study

BACKGROUND: Area-level socioeconomic disparities have long been associated with adverse pregnancy outcomes. Crime is an important element of the neighborhood environment inadequately investigated in the reproductive and public health literature. When crime has been used in research, it has been variably defined, resulting in non-comparable associations across studies. METHODS: Using geocoded linked birth record, crime and census data in multilevel models, this paper explored the relevance of four spatial violent crime exposures: two proximal violent crime categorizations (count of violent crime within a one-half mile radius of maternal residence and distance from maternal residence to nearest violent crime) and two area-level crime categorizations (count of violent crimes within a block group and block group rate of violent crimes) for adverse birth events among women in living in the city of Raleigh NC crime report area in 1999–2001. Models were adjusted for maternal age and education and area-level deprivation. RESULTS: In black and white non-Hispanic race-stratified models, crime characterized as a proximal exposure was not able to distinguish between women experiencing adverse and women experiencing normal birth outcomes. Violent crime characterized as a neighborhood attribute was positively associated with preterm birth and low birth weight among non-Hispanic white and black women. No statistically significant interaction between area-deprivation and violent crime category was observed. CONCLUSION: Crime is variably categorized in the literature, with little rationale provided for crime type or categorization employed. This research represents the first time multiple crime categorizations have been directly compared in association with health outcomes. Finding an effect of area-level violent crime suggests crime may best be characterized as a neighborhood attribute with important implication for adverse birth outcomes