On the design of timber bolted connections subjected to fire

Paper 121Current research at the University of Canterbury is investigating the application of Johansen’s yield equations to the prediction of the failure strength of bolted connections in fire conditions. A series of single bolted connections using steel side plates have been heated at constant temperature for several hours, then loaded to failure. The failure loads have been used to determine the embedment strength of the joints at various temperatures over a range of temperatures from ambient to 300°C. These temperature-dependent embedment strengths have also been used in the Johansen’s equations for wood-steel-wood and wood-wood-wood connections and compared with the results for single bolted connections tested over a range of constant temperatures. Comparisons have also been made with the results of several similar connections tested in fire conditions and show considerable promise for predicting failure of such joints

Ecological changes in historically polluted soils: Metal(loid) bioaccumulation in microarthropods and their impact on community structure

International audienceSoil pollution by persistent metal(loid)s present environmental and sanitary risks. While the effects of metal(loid)s on vegetation and macrofauna have been widely studied, their impact on microarthropods (millimetre scale) and their bioaccumulation capacity have been less investigated. However, microarthropods provide important ecosystem services, contributing in particular to soil organic matter dynamics. This study focussed on the impact of metal(loid) pollution on the structure and distribution of microarthropod communities and their potential to bioaccumulate lead (Pb). Soil samples were collected from a contaminated historical site with a strong horizontal and vertical gradient of Pb concentrations. Microarthropods were extracted using the Berlese method. The field experiments showed that microarthropods were present even in extremely polluted soils (30,000 mg Pb kg− 1). However, while microarthropod abundance increased with increasing soil C/N content (R2 = 0.79), richness decreased with increasing pollution. A shift in the community structure from an oribatid-to a springtail-dominated community was observed in less polluted soils (R2 = 0.68). In addition, Pb bioamplification occurred in microarthropods, with higher Pb concentrations in predators than in detritivorous microarthropods. Finally, the importance of feeding and reproductive ecological traits as potentially relevant descriptors of springtail community structures was highlighted. This study demonstrates the interest of microarthropod communities with different trophic levels and ecological features for evaluating the global environmental impact of metal(loid) pollution on soil biological quality

Bioaccessibility of selenium after human ingestion in relation to its chemical species and compartmentalization in maize

International audienceSelenium is a micronutrient needed by all living organisms including humans, but often present in low concentration in food with possible deficiency. From another side, at higher concentrations in soils as observed in seleniferous regions of the world, and in function of its chemical species, Se can also induce (eco)toxicity. Root Se uptake was therefore studied in function of its initial form for maize (Zea mays L.), a plant widely cultivated for human and animal food over the world. Se phytotoxicity and compartmentalization were studied in different aerial plant tissues. For the first time, Se oral human bioaccessibility after ingestion was assessed for the main Se species (SeIV and SeVI) with the BARGE ex vivo test in maize seeds (consumed by humans), and in stems and leaves consumed by animals. Corn seedlings were cultivated in hydroponic conditions supplemented with 1 mg L−1 of selenium (SeIV, SeVI, Control) for 4 months. Biomass, Se concentration, and bioaccessibility were measured on harvested plants. A reduction in plant biomass was observed under Se treatments compared to control, suggesting its phytotoxicity. This plant biomass reduction was higher for selenite species than selenate, and seed was the main affected compartment compared to control. Selenium compartmentalization study showed that for selenate species, a preferential accumulation was observed in leaves, whereas selenite translocation was very limited toward maize aerial parts, except in the seeds where selenite concentrations are generally high. Selenium oral bioaccessibility after ingestion fluctuated from 49 to 89 % according to the considered plant tissue and Se species. Whatever the tissue, selenate appeared as the most human bioaccessible form. A potential Se toxicity was highlighted for people living in seleniferous regions, this risk being enhanced by the high Se bioaccessibility

Band-filtering in multidimensional NMR

The advantages of band-filtering schemes, based on the selection of an inversion profile, over selective 90° excitation pulse are presented in case of multidimensional NMR spectroscopy. The performances of two "user-friendly" methods based on such a scheme, DANTE-Z and SPIN-PINGING, are discussed. In association with the use of pulse-width-modulated DANTE trains, these methods changes the tedious calibration of band-selective pulses into the calibration of a conventional π/2 "hard" pulse

Soil-plant-atmosphere transfer of organic and metal(oid) pollutants in an industrial and urban area

International audienc

Soil-plant-atmosphere transfer of organic and metal(oid) pollutants in an industrial and urban area

International audienc

Caractérisation et aide à la décision dans le cadre de la gestion d’une ancienne friche industrielle de périphérie urbaine

International audienc

Caractérisation et aide à la décision dans le cadre de la gestion d’une ancienne friche industrielle de périphérie urbaine

International audienc

Chromosomal assignment of the uromodulin gene (UMOD) to 16p13.11

International audienceWe report the chromosomal assignment on chromosome arm 16p of a cDNA clone isolated through its expression in mature kidney and lack of expression in several Wilms tumors. DNA sequencing and analysis of the pattern of RNA expression in different tissues identified this clone as a uromodulin (Tamm-Horsfall glycoprotein, uromucoid; UMOD) sequence. By hybridizing this clone to somatic cell hybrids carrying different human chromosomes or segments of chromosome 16, the gene for UMOD was localized to 16p13.11