Studies on Tumour Active Compounds with Multiple Metal Centres

Four tumour active trinuclear complexes: DH4Cl: [{trans-PtCl(NH3)2}2m-{trans-Pd( NH3)2(H2N(CH2)4NH2)2]Cl4, DH5Cl: [{trans-PtCl(NH3)2}2m-{trans-Pd( NH3)2(H2N(CH2)5NH2)2]Cl4, DH6Cl: [{trans-PtCl(NH3)2}2m-{trans-Pd( NH3)2(H2N(CH2)6NH2)2]Cl4, DH7Cl: [{trans-PtCl(NH3)2}2m-{trans-Pd(NH3)2-( H2N(CH2)7NH2)2]Cl4 and one dinuclear complex DHD: [{trans-PtCl(NH3)2}�-{ H2N(CH2)6NH2}{trans-PdCl(NH3)2]Cl(NO3), have been prepared and characterised based on elemental analyses, IR, Raman, mass and 1 H NMR spectral measurements. For the trinuclear complexes, the synthesis has been carried out using a step-up method branching out from the central palladium unit. A purity of about 95% has been obtained by repeated dissolution and precipitation. The activity against human cancer cell lines including ovary cell lines: A2780, A2780 cisR , A2780 ZD0473R , non small lung cell line: NCI-H640 and melanoma: Me-10538 have been determined based on MMT assay. Cell uptakes, DNA-binding have been determined for ovary cell lines: A2780, A2780 cisR . The nature of interaction with pBR322 plasmid DNA and ssDNA has been studied for trinuclear complexes DH4Cl, DH5Cl, DH6Cl and DH7Cl and the dinuclear complex DHD. Interaction of DH6Cl with adenine and guanine has also been studied by HPLC. The compounds are found to exhibit significant anticancer activity against cancer cell lines especially ovarian cancer cell lines: A2780, A2780 cisR and A2780 ZD0473R . DH6Cl in which the linking diamine has six carbon atoms is found to be the most active compound. As the number of carbon atoms in thelinking diamine is changed from the optimum value of six, the activity is found to decrease, illustrating the structure-activity relationship. The increase in uptake of the trinuclear complexes in A2780 cell line with the increase in size of the linking diamine coupled with the low molar conductivity values found for the solutions of the compounds suggest that the compounds would remain in solution as undissociated �molecules� and hence could cross the cell membrane by passive diffusion. Much lower resistance factors for the all the multinuclear compounds including DHD as applied to A2780 cisR cell line, as compared to that for cisplatin, suggest that the compounds are able to overcome multiple mechanisms of resistance operating in the cell line. All of the multinuclear complexes are expected to form long-range interstrand GG adducts with DNA, causing irreversible global changes in the DNA conformation but unlike cisplatin do not cause sufficient DNA bending to be recognized by HMG 1 protein. Increasing prevention of BamH1 digestion with the increase in concentration of the multinuclear compounds also provide support to the idea that the compounds because of the formation of a plethora of interstrand GG adducts are able to cause irreversible changes in DNA conformation. The results of the study show that indeed new trinuclear tumour active compounds can be found by replacing the central platinum unit in BBR3464 with other suitable metal units

The personality characteristics of emergency nurses

Background: There are ever increasing demands on the emergency nursing workforce so it is necessary to consider how to enhance the recruitment and retention of emergency nurses. Personality is known to influence occupational choice, yet there is a lack of research exploring how personality may influence the workforce decisions of emergency nurses. Aims: To establish the personality profile of a sample of emergency nurses, and to explore whether any relationship exists between their personality characteristics and time spent working within emergency nursing. Methods: A standardised personality assessment instrument, the NEO™-PI-3, was used along with a demographic survey. Data were collected from 72 emergency nurses in an Australian Emergency Department between July and October 2012. Descriptive statistics were used to report demographics and the personality assessment results were compared against general population norms in each of the five personality domains and their 30 associated facets using a one-sample t-test. A two-sided alpha level of .01 was determined to indicate statistical significance. Results: Emergency nurse participants scored higher than the population norms in the domains of extraversion, openness to experience and agreeableness, and in twelve facets, including excitement seeking, vulnerability and competence. Conclusion: The personality profile of this sample of emergency nurses is different from the established population norms. Further research is required to establish whether these study results are applicable to the wider emergency nurse workforce and to establish any link with personality and other nursing specialty choice, which may assist in improving nursing workforce retention and recruitment

Triple-negative breast cancer: Present challenges and new perspectives

Contains fulltext : 83751.pdf (publisher's version ) (Open Access)21 p

Application of spectroscopic imaging and chemometrics for brain tumour classification

Item does not contain fulltextTarragona (Spain) : [S.n.]1 p

Including Spatial Information in Clustering Multivariate Images

Item does not contain fulltextCampinas (Brasil) : [S.n.

Towards tsunami-resistant chemometrics

Contains fulltext : 123095.pdf (publisher's version ) (Open Access

Analytical Sciences: measuring to know and understand

Item does not contain fulltextNijmegen : [S.n.

A self-organizing feature map for clustering nucleic acids - Application to a data matrix containing A-DNA and B-DNA dinucleotides

Contains fulltext : 28238.pdf (publisher's version ) (Open Access

ASPECTS OF PSEUDORANK ESTIMATION METHODS BASED ON THE EIGENVALUES OF PRINCIPAL COMPONENT ANALYSIS OF RANDOM MATRICES

Contains fulltext : 112304.pdf (publisher's version ) (Open Access