Azole fungicide sensitivity and molecular mechanisms of reduced sensitivity in Irish Pyrenopeziza brassicae populations

© 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. This is an open access article under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License. https://creativecommons.org/licenses/by-nc-nd/4.0/BACKGROUND: Light leaf spot, caused by Pyrenopeziza brassicae, is amongst the most damaging diseases of winter oilseed rape(Brassica napus), and currently the sterol 14⊍-demethylase (CYP51) inhibitors (azoles) represent the main class of fungicides used to control light leaf spot development. However, a shift in sensitivity to azole fungicides in P. brassicae populations has been observed in different European countries, including Ireland. RESULTS: To assess the sensitivity status of Irish P. brassicae populations to azole fungicides, three collections of P. brassicae from 2018–2020 were tested in vitro against tebuconazole and prothioconazole-desthio, and the PbCYP51 gene targeted by this class of fungicides was genotyped in different isolates. A change in sensitivity to azole fungicides was observed and differences in sensitivity to tebuconazole between Irish populations were present. There were two substitutions within PbCYP51 (G460Sand S508T) and inserts of different sizes in its promoter region. The presence of the G460S/S508T double mutant was reported for the first time, and the diversity in insert size was greater than previously known. Compared to wild type isolates, those carrying G460S or S508T were less sensitive to both fungicides and, where inserts were also identified, they further reduced sensitivity to azole fungicides. CONCLUSIONS: The results of this study suggest that azole fungicides are still very effective in controlling light leaf spot in Ireland. However, using azole fungicides in mixtures of fungicides with different modes of action is recommendedPeer reviewe

Underwater Object Tracking Using Time Frequency Signatures of Acoustic Signals

International audienceDetecting underwater objects is an important ap plication in marine applications. Most of the techniques are based on the amplitude related techniques, whereby the amplitude of the received echo is used to detect objects within specified bounds. Amplitude techniques however are prone to interference and attenuation, thus limiting the capabilities of such systems. Hence, the aim of this paper is to propose a new technique that detect and track underwater moving objects usingthe turbulence generated by the object. Wideband signals have proven to be a very efficient alternative for merging turbulent flow characteristics and waveform design in order to describe and explain the behavior of turbulence, both artificial and natural. Therefore, constructing adapted waveforms to the natural turbulence embedded in the flow, as well as to the artificial turbulence created by an unknown underwater moving object may hold the key for a new technique for underwater object tracking. When acoustic signals with a particular Instantaneous Frequency Law traveling into underwater environment will hit a moving object, their Instantaneous Frequency Law will capture the object's artificial turbulence, as well as the natural turbulence embedded in the flow. Experimental results carried out in our reduced scale facility provide the validation of the technique

Investigation of molecular mechanisms associated with fungicide sensitivity in Irish Pyrenopeziza brassicae populations

© 2020 The Author(s). This an open access work distributed under the terms of the Creative Commons Attribution Licence (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.Light leaf spot is amongst the most damaging diseases of oilseed rape and a significant threat to Irish crops. Unfortunately, the epidemiology of Pyrenopeziza brassicae, the agent causing this disease, remains poorly understood under Irish growing conditions and fungicides are relied upon to provide control. To investigate if the cropping strategies currently used are the best for the control of disease, we screened three populations of P. brassicae isolates from different regions of Ireland for alterations in the genes targeted by azole and Methyl benzamidazole carbamate fungicides. As molecular mechanisms associated with a decrease in fungicide sensitivity were observed in the populations, the results will be correlated with data from fungicide sensitivity tests using these classes of fungicides.Non peer reviewe

SPIDER4LEGS MOBILE ROBOT DESIGN CONTROLLED BY THE ESP32 PLATFORM

The grazing robot is a dynamic system, consisting of a platform (the body of the robot) and a number of legs with structures similar to the kinematic chains of manipulator robots. Grazing robots can have a redundant number of legs, therefore they can ensure a static stability even if one or more legs are destroyed [10]. The objective of the work is to design a mobile robot controlled with the Arduino board controlled by an ESP 32 logic processor. The robot proposed in the paper is a spider-type robot with four legs, named SPIDER4LEGS. The components used to make the robot are the following: ESP32 development board, ESP ROOM-32, WiFi and Bluetooth BLE, Dual Core; PCA9685 module, I2C interface, 16 CH, servo motor; 12 Servo motors SG90 180 degrees. A possible field of application of these robots is the internal inspection of pipelines transporting petroleum products [7],[8],[9]

On the vortex parameter estimation using wide band signals in active acoustic system

International audienceThis paper proposes a new method for detection of the vortex presence in fluid flow based on an active acoustic system. The experiment that validates the theory was done on a reduced scale facility using ultrasonic transceivers. The main objective was to highlight the effect of a cavitation vortex on an applied wide band signal. In order to accomplish that, the Recurrence Plot Analysis (RPA) was investigated which emhasizes similar states of a dynamic process. The Tests were done from no vortex cavitation flow to vortex cavitation flow and backward

Mental health at different stages of cancer survival: a natural language processing study of Reddit posts

IntroductionThe purpose of this study was to use text-based social media content analysis from cancer-specific subreddits to evaluate depression and anxiety-loaded content. Natural language processing, automatic, and lexicon-based methods were employed to perform sentiment analysis and identify depression and anxiety-loaded content.MethodsData was collected from 187 Reddit users who had received a cancer diagnosis, were currently undergoing treatment, or had completed treatment. Participants were split according to survivorship status into short-term, transition, and long-term cancer survivors. A total of 72524 posts were analyzed across the three cancer survivor groups.ResultsThe results showed that short-term cancer survivors had significantly more depression-loaded posts and more anxiety-loaded words than long-term survivors, with no significant differences relative to the transition period. The topic analysis showed that long-term survivors, more than other stages of survivorship, have resources to share their experiences with suicidal ideation and mental health issues while providing support to their survivor community.DiscussionThe results indicate that Reddit texts seem to be an indicator of when the stressor is active and mental health issues are triggered. This sets the stage for Reddit to become a platform for screening and first-hand intervention delivery. Special attention should be dedicated to short-term survivors

Periodontitis and Heart Disease: Current Perspectives on the Associative Relationships and Preventive Impact

Due to the important advancement and the accumulation of new evidence on the periodontitis-cardiovascular disease (CVD) relationship as well as the major medical, economic and social burden caused by both diseases this chapter aims to review existing epidemiological and pathogenetic links related to this topic. Also, this chapter aims to highlight the impact of the periodontitis-CVD relationships on clinical practice and on the preventive approaches targeting to decrease the impact of periodontitis on CVD. Periodontitis is an infectious disease eliciting local and general inflammation, which leads to periodontal destruction and systemic involvement. Several pathways could explain the link between periodontitis and CVD such as bacteraemia, chronic persistent systemic inflammation and oxidative stress. The first step in the treatment of periodontitis addresses the elimination of microbial components, which lead to a decrease in local and systemic inflammation. Periodontal therapy seems to positively impact CVD. Specialists should inform patients with CVD on the negative impact of periodontitis on their systemic status and refer patients to the periodontist for an extensive examination as routine management of CVD. Some possible risks of periodontal therapy should be considered in patients undergoing antithrombotic medication