Pathogen-induced activation of disease-suppressive functions in the endophytic root microbiome

Microorganisms living inside plants can promote plant growth and health, but their genomic and functional diversity remain largely elusive. Here, metagenomics and network inference show that fungal infection of plant roots enriched for Chitinophagaceae and Flavobacteriaceae in the root endosphere and for chitinase genes and various unknown biosynthetic gene clusters encoding the production of nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs). After strain-level genome reconstruction, a consortium of Chitinophaga and Flavobacterium was designed that consistently suppressed fungal root disease. Site-directed mutagenesis then revealed that a previously unidentified NRPS-PKS gene cluster from Flavobacterium was essential for disease suppression by the endophytic consortium. Our results highlight that endophytic root microbiomes harbor a wealth of as yet unknown functional traits that, in concert, can protect the plant inside out.</p

Celestial calendar-paintings and culture-based digital storytelling: cross-cultural, interdisciplinary, STEM/STEAM resources for authentic astronomy education engagement

In D(L)akota star knowledge, the Sun is known as Wi and the Moon is Han-Wi. They have an important relationship, husband and wife. The pattern of their ever-changing relationship is mirrored in the motions of Sun and Moon as seen from our backyards, also called the lunar phases. The framework of the cultural teaching is storytelling and relationships. Cultural perspectives in astronomy such as this remind us of how indigenous ways of knowing are rooted in inclusion, engagement, and relevancy. Designed by A. Lee in 2007, the Native Skywatchers initiative seeks to remember and revitalize indigenous star and earth knowledge, promoting the native voice as the lead voice. The overarching goal of Native Skywatchers is to communicate the knowledge that indigenous people traditionally practiced a sustainable way of living and sustainable engineering through a living and participatory relationship with the above and below, sky and earth. In 2012 two indigenous star maps were created: the Ojibwe Giizhig Anung Masinaaigan-Ojibwe Sky Star Map (A. Lee, W. Wilson, C. Gawboy), and the D(L)akota star map, Makoce Wicanhpi Wowapi (A. Lee, J. Rock). In 2016, a collaboration with W. Buck of the Manitoba First Nations Resource Centre (MFNRC), produced a third star map: Ininew Achakos Masinikan- Cree Star Map Book. We aim to improve current inequities in education for native young people especially through STEM engagement, to inspire increased cultural pride, and promote community wellness. Presented here will be recently created resources such as: astronomical calendar-paintings and short videos that exist at the intersection of art-science-culture. As we look for sustainable ways to widen participation in STEM, particularly in astronomy education, part of the conversation needs to consider the place for art and culture in STEM

Celestial calendar-paintings and culture-based digital storytelling: cross-cultural, interdisciplinary, STEM/STEAM resources for authentic astronomy education engagement

In D(L)akota star knowledge, the Sun is known as Wi and the Moon is Han-Wi. They have an important relationship, husband and wife. The pattern of their ever-changing relationship is mirrored in the motions of Sun and Moon as seen from our backyards, also called the lunar phases. The framework of the cultural teaching is storytelling and relationships. Cultural perspectives in astronomy such as this remind us of how indigenous ways of knowing are rooted in inclusion, engagement, and relevancy. Designed by A. Lee in 2007, the Native Skywatchers initiative seeks to remember and revitalize indigenous star and earth knowledge, promoting the native voice as the lead voice. The overarching goal of Native Skywatchers is to communicate the knowledge that indigenous people traditionally practiced a sustainable way of living and sustainable engineering through a living and participatory relationship with the above and below, sky and earth. In 2012 two indigenous star maps were created: the Ojibwe Giizhig Anung Masinaaigan-Ojibwe Sky Star Map (A. Lee, W. Wilson, C. Gawboy), and the D(L)akota star map, Makoce Wicanhpi Wowapi (A. Lee, J. Rock). In 2016, a collaboration with W. Buck of the Manitoba First Nations Resource Centre (MFNRC), produced a third star map: Ininew Achakos Masinikan- Cree Star Map Book. We aim to improve current inequities in education for native young people especially through STEM engagement, to inspire increased cultural pride, and promote community wellness. Presented here will be recently created resources such as: astronomical calendar-paintings and short videos that exist at the intersection of art-science-culture. As we look for sustainable ways to widen participation in STEM, particularly in astronomy education, part of the conversation needs to consider the place for art and culture in STEM

Celestial calendar-paintings and culture-based digital storytelling: cross-cultural, interdisciplinary, STEM/STEAM resources for authentic astronomy education engagement

In D(L)akota star knowledge, the Sun is known as Wi and the Moon is Han-Wi. They have an important relationship, husband and wife. The pattern of their ever-changing relationship is mirrored in the motions of Sun and Moon as seen from our backyards, also called the lunar phases. The framework of the cultural teaching is storytelling and relationships. Cultural perspectives in astronomy such as this remind us of how indigenous ways of knowing are rooted in inclusion, engagement, and relevancy. Designed by A. Lee in 2007, the Native Skywatchers initiative seeks to remember and revitalize indigenous star and earth knowledge, promoting the native voice as the lead voice. The overarching goal of Native Skywatchers is to communicate the knowledge that indigenous people traditionally practiced a sustainable way of living and sustainable engineering through a living and participatory relationship with the above and below, sky and earth. In 2012 two indigenous star maps were created: the Ojibwe Giizhig Anung Masinaaigan-Ojibwe Sky Star Map (A. Lee, W. Wilson, C. Gawboy), and the D(L)akota star map, Makoce Wicanhpi Wowapi (A. Lee, J. Rock). In 2016, a collaboration with W. Buck of the Manitoba First Nations Resource Centre (MFNRC), produced a third star map: Ininew Achakos Masinikan- Cree Star Map Book. We aim to improve current inequities in education for native young people especially through STEM engagement, to inspire increased cultural pride, and promote community wellness. Presented here will be recently created resources such as: astronomical calendar-paintings and short videos that exist at the intersection of art-science-culture. As we look for sustainable ways to widen participation in STEM, particularly in astronomy education, part of the conversation needs to consider the place for art and culture in STEM

Targeted Apoptosis of Senescent Cells Restores Tissue Homeostasis in Response to Chemotoxicity and Aging

Accession Number: GSE94395 Platform: GPL16791: Illumina HiSeq 2500 (Homo sapiens) Organism: Homo sapiens Published on 2017-03-01 Summary: The accumulation of irreparable cellular damage restricts healthy lifespan after acute stress or natural aging. Senescent cells are thought to impair tissue function and their genetic clearance can successfully delay features of aging. Identifying how senescent cells avoid apoptosis would allow for the prospective design of anti-senescence compounds to address whether homeostasis can be restored. Here, we identify FOXO4 as a pivot in the maintenance of senescent cell viability. We designed a FOXO4-based peptide which selectively competes for interaction of FOXO4 with p53. In senescent cells, this results in p53 nuclear exclusion and cell-intrinsic apoptosis. Importantly, under conditions where it was well tolerated, the FOXO4 peptide restored liver function after Doxorubicin-induced chemotoxicity. Moreover, in fast aging XpdTTD/TTD, as well as in naturally aged mice the FOXO4 peptide could counteract the loss of fitness, fur density and renal function. Thus, it is possible to therapeutically target senescent cells and thereby effectively counteract senescence-associated loss of tissue homeostasis. Overall Design: mRNA expression levels are compared between IR-induced senescent and proliferating IMR90 cells in triplicate Contact: Name: Peter L.J. de Keizer Organization: Erasmus University Medical Center Laboratory: Senescence in Cancer and Aging Deparment: Molecular Genetics Address: Wytemaweg 80 Rotterdam Netherlands Email: [email protected] Organization: GEO Address: US

Targeted Apoptosis of Senescent Cells Restores Tissue Homeostasis in Response to Chemotoxicity and Aging

Accession Number: GSE94395 Platform: GPL16791: Illumina HiSeq 2500 (Homo sapiens) Organism: Homo sapiens Published on 2017-03-01 Summary: The accumulation of irreparable cellular damage restricts healthy lifespan after acute stress or natural aging. Senescent cells are thought to impair tissue function and their genetic clearance can successfully delay features of aging. Identifying how senescent cells avoid apoptosis would allow for the prospective design of anti-senescence compounds to address whether homeostasis can be restored. Here, we identify FOXO4 as a pivot in the maintenance of senescent cell viability. We designed a FOXO4-based peptide which selectively competes for interaction of FOXO4 with p53. In senescent cells, this results in p53 nuclear exclusion and cell-intrinsic apoptosis. Importantly, under conditions where it was well tolerated, the FOXO4 peptide restored liver function after Doxorubicin-induced chemotoxicity. Moreover, in fast aging XpdTTD/TTD, as well as in naturally aged mice the FOXO4 peptide could counteract the loss of fitness, fur density and renal function. Thus, it is possible to therapeutically target senescent cells and thereby effectively counteract senescence-associated loss of tissue homeostasis. Overall Design: mRNA expression levels are compared between IR-induced senescent and proliferating IMR90 cells in triplicate Contact: Name: Peter L.J. de Keizer Organization: Erasmus University Medical Center Laboratory: Senescence in Cancer and Aging Deparment: Molecular Genetics Address: Wytemaweg 80 Rotterdam Netherlands Email: [email protected] Organization: GEO Address: US