162 research outputs found

    Characterization of Thermophilic Halotolerant Aeribacillus pallidus TD1 from Tao Dam Hot Spring, Thailand

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    The bacterial strain TD1 was isolated from Tao Dam hot spring in Thailand. Strain TD1 was Gram positive, rod-shaped, aerobic, motile, and endospore forming. The cell was 2.0–40 μm in length and about 0.4 μm in diameter. The optimum growth occurred at 55–60 °C and at pH 7–8. Strain TD1 was able to grow on medium containing up to 10% NaCl. The DNA G+C content was 38.9 mol%. The cellular fatty acid content was mainly C16:0, which comprised 25.04% of the total amount of cellular fatty acid. 16S rDNA showed 99% identity to Aeribacillus pallidus DSM 3670T. Bayesian tree analysis strongly supported the idea that strain TD1 is affiliated with genus Aeribacillus, as Aeribacillus pallidus strain TD1. Although the 16S rDNA of A. pallidus strain TD1 is similar to that of A. pallidus DSM 3670T, some physiological properties and the cellular fatty acid profiles differ significantly. A. pallidus strain TD1 can produce extracellular pectate lyase, which has not been reported elsewhere for other bacterial strains in the genus Aeribacillus. A. pallidus strain TD1 may be a good candidate as a pectate lyase producer, which may have useful industrial applications

    Relationship Between Brassiere Cup Size and Shoulder-Neck Pain in Women

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    There are very few reports in regard to relationship between breast size and shoulder-neck pain. The purpose of this study is to examine the correlations among breast size, brassiere cup size, and moment-in-time reporting of shoulderneck pain in a group of adult women. Three hundred thirty nine female volunteers from the hospital staff answered the questionnaire. Breast size, brassiere cup size, and shoulder-neck pain were self-reported by each participant. The relationship among breast size, brassiere cup size and shoulder-neck pain was investigated. Spearman’s test showed no significant relationship between shoulder-neck pain and brassiere cup size. However, after participants were classified into two groups (small brassiere cup size and large brassiere cup size with 219 and 120 participants, respectively), there was a significant positive correlation between shoulder-neck pain and large brassiere cup size (p<0.05). There was no significant relationship between shoulder-neck pain and breast size. In conclusion, large brassiere cup size is an important cause of shoulder-neck pain

    Selection of Anti-Sulfadimidine Specific ScFvs from a Hybridoma Cell by Eukaryotic Ribosome Display

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    BACKGROUND:Ribosome display technology has provided an alternative platform technology for the development of novel low-cost antibody based on evaluating antibiotics derived residues in food matrixes. METHODOLOGY/PRINCIPAL FINDINGS:In our current studies, the single chain variable fragments (scFvs) were selected from hybridoma cell lines against sulfadimidine (SM(2)) by using a ribosome library technology. A DNA library of scFv antibody fragments was constructed for ribosome display, and then mRNA-ribosome-antibody (MRA) complexes were produced by a rabbit reticulocyte lysate system. The synthetic sulfadimidine-ovalbumin (SM(2)-OVA) was used as an antigen to pan MRA complexes and putative scFv-encoding genes were recovered by RT-PCR in situ following each panning. After four rounds of ribosome display, the expression vector pCANTAB5E containing the selected specific scFv DNA was constructed and transformed into Escherichia coli HB2151. Three positive clones (SAS14, SAS68 and SAS71) were screened from 100 clones and had higher antibody activity and specificity to SM(2) by indirect ELISA. The three specific soluble scFvs were identified to be the same molecular weight (approximately 30 kDa) by Western-blotting analysis using anti-E tag antibodies, but they had different amino acids sequence by sequence analysis. CONCLUSIONS/SIGNIFICANCE:The selection of anti-SM(2) specific scFv by in vitro ribosome display technology will have an important significance for the development of novel immunodetection strategies for residual veterinary drugs

    Diagnosis and therapy monitoring of Whipple's arthritis by polymerase chain reaction

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    Diagnosis and therapy monitoring of Whipple's arthritis by polymerase chain reaction

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    Kreativitat von Lehrpersonen als voraussetzung fur kreativitat von schulern und schulerinnen?

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    Поддержка одаренности – развитие креативности : материалы международного конгресса, Витебск, 22–27 сентября 2014 г. : в 2 т. - Витебск : ВГУ имени П.М. Машерова, 2014. - Т. 1. - С. 265-268. - Библиогр.: с. 267-268 (13 назв.).В данной статье исходя из описания значения групповой креативности будут представлены первичные результаты текущего научного проекта «Процессы сотрудничества при групповом преподавании»

    Five Ways In

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    Contact Improvisation first developed in the United States in the 1970s as a movement experiment that challenged dancers to respond instinctively to bodily collisions. Four decades later, the dance form continues to be investigated openly by people all over the world. This film follows the aspirations of five dancers as they navigate the joys and challenges of being with three hundred participants at the biggest Contact Improvisation festival in the world in the old German university town of Freiburg. Jashana, an American political activist and Raquel, a Brazilian volunteer at the festival, search for the political edge and community building potential of the form. Swedish biodynamic gardener, Johan and Israeli travelling teacher, Lior, share hopes for beautiful and challenging dances and new ways to teach the form. Camille from France had only recently discovered the form. He seeks the heart of Contact Improvision out of his comfort zone of salsa and sports in the pursuit of sensitivity and weight sharing. During a week where being part of a temporary community and the intimacy and physicality of the form can challenge even experienced dancers, will the Freiburg Festival deliver the inspiration and insights they seek
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