Regulation of MicroRNA Biogenesis: A miRiad of mechanisms

microRNAs are small, non-coding RNAs that influence diverse biological functions through the repression of target genes during normal development and pathological responses. Widespread use of microRNA arrays to profile microRNA expression has indicated that the levels of many microRNAs are altered during development and disease. These findings have prompted a great deal of investigation into the mechanism and function of microRNA-mediated repression. However, the mechanisms which govern the regulation of microRNA biogenesis and activity are just beginning to be uncovered. Following transcription, mature microRNA are generated through a series of coordinated processing events mediated by large protein complexes. It is increasingly clear that microRNA biogenesis does not proceed in a 'one-size-fits-all' manner. Rather, individual classes of microRNAs are differentially regulated through the association of regulatory factors with the core microRNA biogenesis machinery. Here, we review the regulation of microRNA biogenesis and activity, with particular focus on mechanisms of post-transcriptional control. Further understanding of the regulation of microRNA biogenesis and activity will undoubtedly provide important insights into normal development as well as pathological conditions such as cardiovascular disease and cancer

A 19 to 17 Ma amagmatic extension event at the Mid-Atlantic Ridge: Ultramafic mylonites from the Vema Lithospheric Section

A >300 km long lithospheric section (Vema Lithospheric Section or VLS) is exposed south of the Vema transform at 11 degrees N in the Atlantic. It is oriented along a seafloor spreading flow line and represents similar to 26 Ma of accretion at a single 80 km long segment (EMAR) of the Mid-Atlantic Ridge. The basal part of the VLS exposes a mantle unit made mostly of relatively undeformed coarse-grained/porphyroclastic peridotites that were sampled at close intervals. Strongly deformed mylonitic peridotites were found at 14 contiguous sites within a similar to 80 km stretch (similar to 4.7 Ma interval); they are dominant in a time interval of 1.4 Ma, from crustal ages of 16.8 to 18.2 Ma (mylonitic stretch). Some of the mylonites are "dry," showing anhydrous high-T deformation, but most contain amphibole. The mylonitic peridotites tend to be less depleted than the porphyroclastic peridotites on the basis of mineral major and trace elements composition, suggesting that the mylonites parent was a subridge mantle that underwent a relatively low degree of melting. The Sr, Nd, and O isotopic composition of the amphiboles is MORB-like and suggests either that seawater did not contribute to their isotopic signature or that their isotopic ratios re-equilibrated during fluid circulation in the upper mantle. Four (40)Ar/(39)Ar ages, on three amphiboles separated from the peridotites, are close to crustal ages predicted from magnetic anomalies, confirming that the amphiboles formed close to ridge axis. We propose that crustal accretion at the EMAR segment has been mostly symmetrical for the 26 Ma of its recorded history, except for the similar to 1.4 Ma interval of prevalent ultramafic mylonites (mylonitic stretch) that may record a period of quasi-amagmatic asymmetric accretion of oceanic lithosphere close to the ridge-Vema transform intersection, possibly with development of detachment faults. This interval may correspond to a thermal minimum of the subridge upwelling mantle, marking the transition from a period of decreasing to one of increasing mantle melting below the EMAR segment

Deep origin and hot melting of an Archaean orogenic peridotite massif in Norway

The buoyancy and strength of sub-continental lithospheric mantle is thought to protect the oldest continental crust (cratons) from destruction by plate tectonic processes. The exact origin of the lithosphere below cratons is controversial, but seems clearly to be a residue remaining after the extraction of large amounts of mel