Ecophysiological Responses of Two Olive Tree Cultivars (Olea europaea L. CV Koroneiki and Chemlali) Under Three Water Treatments

In this paper, our objective is to assess and compare the effects of three levels of irrigation application on the ecophysiological behavior and plant dry matter accumulation of two young olive tree cultivars Koroneiki, a promising greek cultivar, and Chemlali, the best local cultivar. According to this assessment, we can determine the most efficient water treatment that could be suitable for Mediterranean environments subjected to water shortage conditions. Measurements were made in the green house of the Tunisian Olive tree Institute under normal day-light conditions from March, 16th to April, 21st 2015. Three water treatments were applied which are T100% (control treatment: Daily irrigation at 100% of Available Water Content (AWC)), T50% (Daily irrigation at 50% of AWC) and T0% (Without watering). The results showed that the two olive tree cultivars possess important mechanisms to overtake limited water resources. However, some striking variations existed between the two cultivars studied. Indeed, as water stress increased (T0%), Chemlali maintained longer a high midday leaf water potential compared (- 4.54 MPa) to Koroneiki (- 5.8 MPa). T50% treatment seems to be sufficient for both cultivars. Measurements of total Osmotic Adjustment (OA) showed that olive trees use this mechanism to create very negative leaf water potentials in order to be able to extract water from a dry root environment. The root/shoot ratio of Chemlali plants at T50% treatment was the highest (1.08) comparatively with the other two treatments (0.70 and 0.79 for T100% and T0% AWC water treatments, respectively). This result shows that Chemlali plants valorize low quantities of water (T50%) rather than high quantities (T100%) and Koroneiki plants behave better when it is irrigated at 100% AWC. To conclude, Chemlali plants irrigated at 50% AWC, compared to Koroneiki plants, are the most suitable to tolerate water restriction conditions

Genetic landscape of a large cohort of Primary Ovarian Insufficiency : New genes and pathways and implications for personalized medicine

Background Primary Ovarian Insufficiency (POI), a public health problem, affects 1-3.7% of women under 40 yield-ing infertility and a shorter lifespan. Most causes are unknown. Recently, genetic causes were identified, mostly in single families. We studied an unprecedented large cohort of POI to unravel its molecular pathophysiology.Methods 375 patients with 70 families were studied using targeted (88 genes) or whole exome sequencing with pathogenic/likely-pathogenic variant selection. Mitomycin-induced chromosome breakages were studied in patients' lymphocytes if necessary. Findings A high-yield of 29.3% supports a clinical genetic diagnosis of POI. In addition, we found strong evidence of pathogenicity for nine genes not previously related to a Mendelian phenotype or POI: ELAVL2, NLRP11, CENPE, SPATA33, CCDC150, CCDC185, including DNA repair genes: C17orf53(HROB), HELQ, SWI5 yielding high chromo-somal fragility. We confirmed the causal role of BRCA2, FANCM, BNC1, ERCC6, MSH4, BMPR1A, BMPR1B, BMPR2, ESR2, CAV1, SPIDR, RCBTB1 and ATG7 previously reported in isolated patients/families. In 8.5% of cases, POI is the only symptom of a multi-organ genetic disease. New pathways were identified: NF-kB, post-translational regulation, and mitophagy (mitochondrial autophagy), providing future therapeutic targets. Three new genes have been shown to affect the age of natural menopause supporting a genetic link.Interpretation We have developed high-performance genetic diagnostic of POI, dissecting the molecular pathogene-sis of POI and enabling personalized medicine to i) prevent/cure comorbidities for tumour/cancer susceptibility genes that could affect life-expectancy (37.4% of cases), or for genetically-revealed syndromic POI (8.5% of cases), ii) predict residual ovarian reserve (60.5% of cases). Genetic diagnosis could help to identify patients who may benefit from the promising in vitro activation-IVA technique in the near future, greatly improving its success in treating infertility.Funding Universite? Paris Saclay, Agence Nationale de Biome?decine.Copyright (c) 2022 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)Peer reviewe

Quelle technique adopter pour le phénotypage des alvéolites lymphocytaires : immunocytochimie ou cytométrie en flux ?

International audienceBackground : Diffuse interstitial pneumonias are considered as a group of multiple affections characterized by challenging diagnoses because of the lack of specific clinical signs. Radiologic investigations highlight the diagnoses in most of the cases but bronchoalveolar lavage plays a key role in the diagnostic diagram. We aim to compare the immunocytochemical technique and the flow cytometry in the phenotyping of lymphocytic alveolitis.Methods : We described a series of 32 lymphocytic alveolitis, which were analyzed using immunocytochemistry and flow cytometry.Results : We found a good reproducibility between the immunocytochemistry performed on smears and cytoblocks (kappa = 0.7) and a poor reproducibility between immunocytochemistry and flow cytometry (kappa = 0.35).Conclusion : Our study emphasized on the poor reproducibility between immunocytochemistry and flow cytometry. Further studies about the reliability of both techniques are needed especially in discordant cases.Introduction : Les pneumopathies interstitielles diffuses sont considérées comme un groupe d’affections multiples diverses et de diagnostic difficile vu la non-spécificité des signes cliniques. Les moyens d’imagerie orientent dans la plupart des cas vers le diagnostic, cependant, le lavage bronchoalvéolaire garde toute son importance dans les cas peu spécifiques. Notre objectif est de comparer l’immunocytochimie et la cytométrie en flux dans le phénotypage des alvéolites lymphocytaires.Matériel et méthodes : Nous avons colligé 32 cas d’alvéolites lymphocytaires diagnostiquées et prises en charge au service d’anatomie pathologique pour l’analyse cytologique du LBA et le marquage immunocytochimique et dans le département d’hématologie (unité de cytométrie en flux) pour le phénotypage par cytométrie en flux.Résultats : Nous avons mis en évidence une bonne reproductibilité entre l’immunocytochimie sur étalements et sur cytoblocs (kappa = 0,7) tandis que l’immunocytochimie et la cytométrie en flux semblaient peu comparables (kappa = 0,35).Conclusion : Notre étude met l’accent sur la mauvaise reproductibilité entre l’immunocytochimie et la cytométrie en flux. Nous projetons de nous référer aux diagnostics cliniques retenus afin d’asseoir les résultats discordants entre les 2 techniques et de mettre en évidence leur fiabilité et justesse

Ecophysiological and biochemical aspects of olive tree (Olea europaea L.) in response to salt stress and gibberellic acid-induced alleviation

Soil salinization is considered as one of the most important risks for agricultural soils. The objective of this experiment was to study the ecophysiological and the biochemical behaviour of two cultivars of Olea europaea L., 'Chemlali' and 'Koroneiki' under two salinity levels (100 and 200mM NaCl) and the potential alleviation induced by gibberellic acid (GA3) foliar sprays. Salinity treatments significantly decreased photosynthetic assimilation rate and stomatal conductance compared to the control for both cultivars, but 'Chemlali' showed a higher resistance to increasing NaCl salinity compared to 'Koroneiki'. Leaf chlorophyll index also reduced gradually with increasing salinity concentration compared to the control. At the end of the experiment, a decrease in growth and dry matter accumulation was observed. Under high salinity stress, a significant decrease in root DW was recorded by 37% and 59% for 'Chemlali' and 'Koroneiki', respectively. High salinity stress decreased also shoot DW up to 51% for 'Chemlali'. However, mannitol concentration increased under increasing salinity levels compared to control for 'Chemlali' cultivars. Interestingly, foliar application of GA3 alleviated the negative effects of salinity on ecophysiological parameters especially for 'Koroneiki'. Indeed, GA3 improved photosynthetic assimilation up to 14% for 'Chemlali' and 36% for 'Koroneiki' compared to high salinity treatment. Both cultivars showed an increase in leaf chlorophyll index after applying GA3. Under high salinity combined with GA3, growth and dry weight were increased compared to salt stressed plants without GA3. The obtained results report that 'Chemlali' cultivar is more tolerant to salinity than 'Koroneiki' and suggest that GA3 plays an important role to reduce negative effects of NaCl salinity

Analysis of Outcomes in Ischemic vs Nonischemic Cardiomyopathy in Patients With Atrial Fibrillation A Report From the GARFIELD-AF Registry

IMPORTANCE Congestive heart failure (CHF) is commonly associated with nonvalvular atrial fibrillation (AF), and their combination may affect treatment strategies and outcomes