4,309 research outputs found
Structural features and domain movements controlling substrate binding and cofactor specificity in class II HMG-CoA reductase
The key mevalonate pathway enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (HMGR) uses the cofactor NAD(P)H to reduce HMG-CoA to mevalonate in the production of countless metabolites and natural products. Although inhibition of HMGR by statin drugs is well-understood, several mechanistic details of HMGR catalysis remain unresolved, and the structural basis for the wide range of cofactor specificity for either NADH or NADPH among HMGRs from different organisms is also unknown. Here, we present crystal structures of HMGR from Streptococcus pneumoniae (SpHMGR) alongside kinetic data of the enzyme’s cofactor preferences. Our structure of SpHMGR bound with its kinetically preferred NADPH cofactor suggests how NADPH-specific binding and recognition are achieved. In addition, our structure of HMG-CoA-bound SpHMGR reveals large, previously unknown conformational domain movements that may control HMGR substrate binding and enable cofactor exchange without intermediate release during the catalytic cycle. Taken together, this work provides critical new insights into both the HMGR reaction mechanism and the structural basis of cofactor specificity
Structural features and domain movements controlling substrate binding and cofactor specificity in class II HMG-CoA reductase
The key mevalonate pathway enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (HMGR) uses the cofactor NAD(P)H to reduce HMG-CoA to mevalonate in the production of countless metabolites and natural products. Although inhibition of HMGR by statin drugs is well-understood, several mechanistic details of HMGR catalysis remain unresolved, and the structural basis for the wide range of cofactor specificity for either NADH or NADPH among HMGRs from different organisms is also unknown. Here, we present crystal structures of HMGR from Streptococcus pneumoniae (SpHMGR) alongside kinetic data of the enzyme’s cofactor preferences. Our structure of SpHMGR bound with its kinetically preferred NADPH cofactor suggests how NADPH-specific binding and recognition are achieved. In addition, our structure of HMG-CoA-bound SpHMGR reveals large, previously unknown conformational domain movements that may control HMGR substrate binding and enable cofactor exchange without intermediate release during the catalytic cycle. Taken together, this work provides critical new insights into both the HMGR reaction mechanism and the structural basis of cofactor specificity
Evidence for High-Frequency QPOs with a 3:2 Frequency Ratio from a 5000 Solar Mass Black Hole
Following the discovery of 3:2 resonance quasi-periodic oscillations (QPOs)
in M82X-1 (Pasham et al. 2014), we have constructed power density spectra (PDS)
of all 15 (sufficiently long) {\it XMM-Newton} observations of the
ultraluminous X-ray source NGC1313X-1 ( 210
erg/sec). We detect a strong QPO at a frequency of 0.290.01 Hz in data
obtained on 2012 December 16. Subsequent searching of all the remaining
observations for a 3:2/2:3 frequency pair revealed a feature at 0.460.02
Hz on 2003 Dec 13 (frequency ratio of 1.590.09). The global significance
of the 0.29 Hz feature considering all frequencies between 0.1 and 4 Hz is
3.5 . The significance of the 0.460.02 Hz QPO is 3.5
for a search at 2/3 and 3/2 of 0.29 Hz. We also detect lower frequency QPOs
(32.92.6 and 79.71.2 mHz). All the QPOs are super-imposed on a
continuum consisting of flat-topped, band-limited noise, breaking into a
power-law at a frequency of 163 mHz and white noise at 0.1 Hz.
NGC1313X-1's PDS is analogous to stellar-mass black holes' (StMBHs) PDS in the
so-called steep power-law state, but with the respective frequencies (both QPOs
and break frequencies) scaled down by a factor of 1000. Using the
inverse mass-to-high-frequency QPO scaling of StMBHs, we estimate NGC1313X-1's
black hole mass to be 50001300 , consistent with an inference
from the scaling of the break frequency. However, the implied Eddington ratio,
L 0.030.01, is significantly lower compared to StMBHs in the
steep power-law state (L 0.2).Comment: Published in ApJ Letter
On Neutral Absorption and Spectral Evolution in X-ray Binaries
Current X-ray observatories make it possible to follow the evolution of
transient and variable X-ray binaries across a broad range in luminosity and
source behavior. In such studies, it can be unclear whether evolution in the
low energy portion of the spectrum should be attributed to evolution in the
source, or instead to evolution in neutral photoelectric absorption. Dispersive
spectrometers make it possible to address this problem. We have analyzed a
small but diverse set of X-ray binaries observed with the Chandra High Energy
Transmission Grating Spectrometer across a range in luminosity and different
spectral states. The column density in individual photoelectric absorption
edges remains constant with luminosity, both within and across source spectral
states. This finding suggests that absorption in the interstellar medium
strongly dominates the neutral column density observed in spectra of X-ray
binaries. Consequently, evolution in the low energy spectrum of X-ray binaries
should properly be attributed to evolution in the source spectrum. We discuss
our results in the context of X-ray binary spectroscopy with current and future
X-ray missions.Comment: Accepted for publication in ApJ Letter
The Specific Globular Cluster Frequencies of Dwarf Elliptical Galaxies from the Hubble Space Telescope
The specific globular cluster frequencies (S_N) for 24 dwarf elliptical (dE)
galaxies in the Virgo and Fornax Clusters and the Leo Group imaged with the
Hubble Space Telescope are presented. Combining all available data, we find
that for nucleated dEs --- which are spatially distributed like giant
ellipticals in galaxy clusters --- S_N(dE,N)=6.5 +- 1.2 and S_N increases with
M_V, while for non-nucleated dEs --- which are distributed like late-type
galaxies --- S_N(dE,noN)=3.1 +- 0.5 and there is little or no trend with M_V.
The S_N values for dE galaxies are thus on average significantly higher than
those for late-type galaxies, which have S_N < 1. This suggests that dE
galaxies are more akin to giant Es than to late-type galaxies. If there are
dormant or stripped irregulars hiding among the dE population, they are likely
to be among the non-nucleated dEs. Furthermore, the similarities in the
properties of the globular clusters and in the spatial distributions of dE,Ns
and giant Es suggest that neither galaxy mass or galaxy metallicity is
responsible for high values of S_N. Instead, most metal-poor GCs may have
formed in dwarf-sized fragments that merged into larger galaxies.Comment: 12 pages (uses aaspp4.sty), 2 figures, 1 table, to appear in the
Astrophysical Journa
Petrov types of slowly rotating fluid balls
Circularly rotating axisymmetric perfect fluid space-times are investigated
to second order in the small angular velocity. The conditions of various
special Petrov types are solved in a comoving tetrad formalism. A number of
theorems are stated on the possible Petrov types of various fluid models. It is
shown that Petrov type II solutions must reduce to the de Sitter spacetime in
the static limit. Two space-times with a physically satisfactory
energy-momentum tensor are investigated in detail. For the rotating
incompressible fluid, it is proven that the Petrov type cannot be D. The
equation of the rotation function can be solved for the Tolman type
IV fluid in terms of quadratures. It is also shown that the rotating version of
the Tolman IV space-time cannot be Petrov type D.Comment: 14 pages, version to appear in Gen. Rel. Gra
Magnetic field dependence of the internal quality factor and noise performance of lumped-element kinetic inductance detectors
We present a technique for increasing the internal quality factor of kinetic
inductance detectors (KIDs) by nulling ambient magnetic fields with a properly
applied magnetic field. The KIDs used in this study are made from thin-film
aluminum, they are mounted inside a light-tight package made from bulk
aluminum, and they are operated near . Since the thin-film
aluminum has a slightly elevated critical temperature (), it therefore transitions before the package (), which also serves as a magnetic shield. On cooldown, ambient
magnetic fields as small as approximately can produce
vortices in the thin-film aluminum as it transitions because the bulk aluminum
package has not yet transitioned and therefore is not yet shielding. These
vortices become trapped inside the aluminum package below
and ultimately produce low internal quality factors in the thin-film
superconducting resonators. We show that by controlling the strength of the
magnetic field present when the thin film transitions, we can control the
internal quality factor of the resonators. We also compare the noise
performance with and without vortices present, and find no evidence for excess
noise beyond the increase in amplifier noise, which is expected with increasing
loss.Comment: 5 pages, 4 figure
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