Determination of Different Biological Factors on the Base of Dried Blood Spot Technology

It is well-known that distinct biological indices (analytes) have distinct variability. We try to use some mathematical algorithms to pick out a set of blood parameters which give an opportunity to retrieve the initial volume of the blood spotted, and use it to calculate exact concentrations of analyts interesting to a physician. For our analysis we used the database of biochemical blood parameters obtained in Russian Scientific Center of Roentgen-Radiology during 1995-2000, which includes more than 30000 of patients.Comment: 5 page

Creating a Database of Helicopter Main Rotor Acoustics for Validation of CFD Methods

The work presents recent experiments at the Kazan National Technical University (KNRTU-KAI), related to helicopter acoustics. The objective is to provide a database of near-field experimental data suitable for CFD validation. The obtained set of data corresponds to a Mach-scaled rotor of known planform. An advantage of the current dataset is that direct near-field acoustic data is made available and this allows easy and direct comparisons with CFD predictions, without the need to use far-field aeroacoustic methods

Ruthenibacterium lactatiformans gen. nov., sp.nov., an anaerobic, lactate-producing member of the family Ruminococcaceae isolated from human faeces

Two novel strains of Gram-stain-negative, rod-shaped, obligately anaerobic, non-spore-forming, non-motile bacteria were isolated from the faeces of healthy human subjects. The strains, designated as 585-1T and 668, were characterized by mesophilic fermentative metabolism, production of d-lactic acid, succinic acid and acetic acid as end products of d-glucose fermentation, prevalence of C18 : 1 ω9, C18 : 1 ω9 aldehyde, C16 : 0 and C16 : 1 ω7c fatty acids, presence of glycine, glutamic acid, lysine, alanine and aspartic acid in the petidoglycan peptide moiety and lack of respiratory quinones. Whole genome sequencing revealed the DNA G+C content was 56.4–56.6 mol%. The complete 16S rRNA gene sequences of the two strains shared 91.7/91.6 % similarity with Anaerofilum pentosovorans FaeT, 91.3/91.2 % with Gemmiger formicilis ATCC 27749T and 88.9/88.8 % with Faecalibacterium prausnitzii ATCC 27768T. On the basis of chemotaxonomic and genomic properties it was concluded that the strains represent a novel species in a new genus within the family Ruminococcaceae , for which the name Ruthenibacterium lactatiformans gen. nov., sp. nov. is proposed. The type strain of Ruthenibacterium lactatiformans is 585-1T (=DSM 100348T=VKM B-2901T)

Оцінювання запасів прісної води півдня України

The method of determining available water resources and the volumes of their use are presented. The methodology uses indicators: water quantity and quality, variability, raising public awareness of water problems, access to water and vulnerability of ecosystems. On the basis of the «Atlas of Water Risk» (interactive map), based on the described method, an assessment of the availability of fresh water in southern regions of Ukraine was made using the example of data for the year 2015.Наведено методику визначення доступних водних ресурсів і обсягів їх використання за показниками кількості води, її мінливості та якості, підвищення обізнаності громадськістю про водні проблеми, доступу до води і уразливості екосистем. На базі «Атласу водного ризику» (інтерактивної карти) за описаною методикою проведено оцінювання забезпеченості південних регіонів України прісною водою на прикладі даних 2015 р

Measurement of intracellular concentration of fluorescently-labeled targets in living cells

Estimations of intracellular concentrations of fluorescently-labeled molecules within living cells are very important for guidance of biological experiments and interpretation of their results. Here we propose a simple and universal approach for such estimations. The approach is based upon common knowledge that the dye fluorescence is directly proportional to its quantum yield and the number of its molecules and that a coefficient of proportionality is determined by spectral properties of the dye and optical equipment used to record fluorescent signals. If two fluorescent dyes are present in the same volume, then a ratio of their concentrations is equal to a ratio of their fluorescence multiplied by some dye- and equipment-dependent coefficient. Thus, if the coefficient and concentration of one dye is known then the concentration of another dye can be determined. Here we have demonstrated how to calculate this coefficient (called a ratio factor) and how to use it for concentration measurements of fluorescently tagged molecules. As an example of how this approach can be used, we estimated a concentration of exogenously expressed neuronal Ca2+ sensor protein, hippocalcin, tagged by a fluorescent protein in a dendritic tree of rat hippocampal neurons loaded via a patch pipette with Alexa Fluor dye of known concentration. The new approach should allow performing a fast, inexpensive and reliable quantitative analysis of fluorescently-labeled targets in different parts of living cells

Оптимизация уровня собственного удержания риска на страховом рынке

In this paper, we discuss key aspects of net retention ratio optimization due to risk transfer for reinsurance as a factor of financial stability and security in the insurance company. A net retention ratio algorithm, which should be the responsibility of the insurance company for ensuring the minimum required level of the insurer’s financial security, was developed for this study. The implementation of suggested approach is performed based at economic and mathematical model («linear programming»), subject to the insurance market performance.У статті розглянуто ключові аспекти оптимізації частки власного утримання ризику страховою компанією (СК) у процесі його передачі в перестрахування як фактору забезпечення фінансової стабільності та безпеки СК. Для цього дослідження було розроблено алгоритм, що дозволяє визначити частку власного утримання, яка повинна зали- шатися на відповідальності страхової компанії для забезпечення мінімально необхідного рівня фінансової безпеки страховика. Запропонований авторами підхід, що ґрунтується на результатах економіко-математичного моделюван- ня, проходить практичну апробацію в ряді СК України.В статье рассмотрены ключевые аспекты оптимизации собственного удержания страховой компанией (СК) риска в процессе его передачи в перестрахование как фактора обеспечения финансовой стабильности и безо- пасности СК. Для данного исследования был разработан алгоритм определения доли собственного удержания, которая должна оставаться на ответственности страховой компании для обеспечения минимально необходимого уровня финансовой безопасности страховщика. Предложенный авторами подход, который базируется на результатах экономико-математического моделирования, проходит практическую апробацию в ряде СК Украины

Role of viral infection in the etiopathogenesis of breast cancer

The viral nature of many female genital cancers is now beyond question; however, the role of viral infection in the pathogenesis of breast cancer (BC) has not been adequately investigated. The paper defines the importance of a number of viruses in the etiopathogenesis of on- cogynecological diseases. It presents the results of examining 60 patients with Stages I-IV BC and 30 patients with fibrocystic mastopathy, in whom the presence of DNA-containing virus genomes in tumor tissue was compared, and the data of polymerase chain reaction study of genital tract smears. It is shown that human papillomaviruses and cytomegaloviruses do not play a fundamental role in the develop- ment of BC; there is no valid evidence for Epstein–Barr virus

The Specificity of the Differential Regulation of Economic Integration in the Context of Contemporary Labor Migration

The issues of integration in the modern world become relevant in the rapidly changing geopolitical realities, and the strategic imperative of time for the vast majority of States. Today in accordance with the principle of multi-level and various-speed integration on Eurasian space are successfully developing various integration formats. They complement each other to provide a wide partnership platform. The authors argue that the main participants in labor migration in the post-Soviet space reflect the actual path of social and economic interaction worked out historically. Common historical fate and similar paths of development, similar mental conceptosphere steel trigger mechanism for the implementation of the Eurasian Economic Union. Researches show that labour of migrant workers from different countries is widely distributed in different sectors of Russia's economy, their labour is used almost everywhere. In many industries a mechanism was established where officially Russian workers are registered at enterprises, but foreign migrant workers are actually employed. Russians began to actively move, migration processes have increased substantially, new forms of temporary migration. These processes have become more active throughout Eurasia. The authors analyze the causes and characteristics of migration in the Eurasian economic union at the present stage. DOI: 10.5901/mjss.2015.v6n3p9

НОВЫЕ МЕТОДЫ ПРОГНОЗА ЧУВСТВИТЕЛЬНОСТИ РАКА ПОЧКИ К ИММУНОТЕРАПИИ НА ОСНОВЕ АНАЛИЗА МОЛЕКУЛЯРНО-ГЕНЕТИЧЕСКИХ ХАРАКТЕРИСТИК ОПУХОЛИ

The paper reviews the literature on the treatment of renal cancer, mainly on postoperative immunotherapy. It outlines a role of cytokines in tumor-immune system interaction, the prognostic value of the level of expression of each cytokine for both survival rates and the efficiency of immunotherapy, as well as the use of this information to develop new therapeutic approaches.Приведен обзор литературы, посвещенный проблеме лечения рака почки, главным образом проведению послеоперационной иммунотерапии. Освещается роль цитокинов во взаимодействии опухоли и иммунной системы, определена прогностическая ценность величины экспрессии каждого цитокина в отношении как выживаемости пациента, так и эффективности иммунотерапии, а также использования этой информации для разработки новых терапевтических подходов

MOLECULAR-GENETIC CHARACTERISTICS OF PRIMARY TUMOR AND METASTATIC LYMPHATIC NODES IN BREAST CANCER

The purpose of systemic treatment in patients with breast cancer is based largely on the molecular characteristics of the primary tumor, but many clinical recommendations suggest also the study of metastatic nodes with an assessment of their receptor status (estrogen receptor ER, progesterone receptor RP, human epidermal growth factor receptor 2 Her2/neu). This is due to the fact that according to numerous studies, the discrepancy between the status of the primary tumor and the secondary nodes can reach high rates: 3–54 % for ER, 5–78 % for RP, and 0–34 % for Her2/neu. At the same time, more and more data actively demonstrate the imperfection of immunohistochemical analysis and the need to study additional parameters to improve the quality of diagnosis of patients with breast cancer. Material and methods. A morphological and immunohistochemical study of the tumor tissue of the primary node and axillary lymph nodes was performed in 199 patients with breast cancer (T1-3N0-3M0) using standard methods, and RT-PCR was also studied with the expression of 24 genes. Results. The incidence of differences between the molecular phenotypes of the main tumor and metastatic axillary lymph nodes was 26 (26 %) of 99 cases. Most often, differences were noted in cases of breast cancer with luminal A type – 13 cases (50 %). According to the results of a comparative PCR analysis of tissue samples from the primary tumor and metastatic regional lymph nodes, only the expression of the CD68, ERSR1, GRB7 and MMD11 receptors was statistically significant. Conclusion. The results indicate the need for an integrated approach and additional methods for the diagnosis of breast cancer, which will undoubtedly improve the quality of planning and the effectiveness of systemic treatment in patients with breast cancer