Testicular endocrine activity is upregulated by D-Aspartic acid in the green frog Rana esculenta

This study investigated the involvement of D-aspartic acid (D-Asp) in testicular steroidogenesis of the green frog Rana esculenta and its effect on stimulation of thumb pad morphology and glandular activity, a typical testosterone-dependent secondary sexual characteristic in this amphibian species. In the testis, D-Asp concentrations vary significantly during the reproductive cycle: they are low in pre- and post-reproductive periods, but reach peak levels in the reproductive period (140-236 nmol/g wet tissue). Moreover, the concentrations of D-Asp in the testis through the sexual cycle positively match the testosterone levels in the gonad and the plasma. The racemase activity evaluated during the cycle expresses its peak when D-Asp and testosterone levels are highest, that is, during the reproductive period, confirming the synthesis of D-Asp from L-Asp by an aspartate racemase. Short-term in vivo experiments consisting of a single injection of D-Asp (2.0 micro mol/g body weight) demonstrated that this amino acid accumulates significantly in the testis, and after 3 h its uptake is coupled with a testosterone increase in both testis and plasma. Moreover, within 18 h of amino acid administration, the D-Asp concentration in the testis decreased along with the testosterone titer to prestimulation levels. Other amino acids (L-Asp, D-Glu and L-Glu) used instead of D-Asp were ineffective, confirming that the significant increase in testicular testosterone was a specific feature of this amino acid. In long-term experiments, D-Asp had been administered chronically to frogs caught during the three phases of the reproductive cycle, inducing testosterone increase and 17beta-estradiol decrease in the gonad during the pre- and post-reproductive period, and vice versa during the reproductive period

Statutory Comment

International audienc

Exploring NLS1 through the physical properties of their hosts

In this work we aim at addressing the still open question about the nature of Narrow-Line Seyfert 1 (NLS1) galaxies: are they really active nuclei with lower mass Black-Holes (BHs) than Seyfert 1 (S1) and quasars? Our approach is based on the recently discovered physical connections between nuclear supermassive BHs and their hosting spheroids (spiral bulges or ellipticals). In particular we compare BH masses of NLS1s and S1s analyzing the properties of their hosts by means of spectroscopic and photometric data in the optical wavelength domain. We find that NLS1s fill the low BH mass and bulge luminosity values of the BH mass - bulge magnitude relation, a result strongly suggesting that NLS1s are active nuclei where less massive BHs are hosted by less massive bulges. The correlation is good with a relatively small scatter fitting simultaneously NLS1s, S1s and quasars. On the other hand, NLS1s seem to share the same stellar velocity dispersion range of S1s in the BH mass - stellar velocity dispersion relation, indicating that NLS1s have a smaller BH/bulge mass ratio than S1s. These two conflicting results support in any case the idea that NLS1s could be young S1s. Finally we do not confirm the significantly non linear BH--bulge relation claimed by some authors

Stellar velocity dispersion in NLS1 galaxies

Several authors recently explored the Black-Hole mass (M_BH) vs. stellar velocity dispersion (sigma_*) relationship for Narrow Line Seyfert 1 galaxies (NLS1s). Their results are more or less in agreement and seem to indicate that NLS1s fill the region below the fit obtained by Tremaine et al. (2002), showing a range of sigma_* similar to that of Seyfert 1 galaxies, and a lower M_BH. Until now the [O III] width has been used in place of the stellar velocity dispersion, but some indications begin to arise against the effectiveness of the gaseous kinematics in representing the bulge potential at least in NLS1s. Bian & Zhao (2004) stressed the urgency to produce true sigma_* measurements. Here we present new stellar velocity dispersions obtained through direct measurements of the Ca II absorption triplet (~ 8550 A) in the nuclei of 8 NLS1 galaxies. The resulting sigma_* values and a comparison with sigma_[O III] confirm our suspects (see Botte et al. 2004) that [O III] typically overestimates the stellar velocity dispersion and demonstrate that NLS1s follow the M_BH-sigma_* relation as Seyfert 1, quasars and non-active galaxies.Comment: 5 pages, 4 figures, accepted for publication in MNRA

Gulf of Naples Advanced Model (GNAM): A Multiannual Comparison with Coastal HF Radar Data and Hydrological Measurements in a Coastal Tyrrhenian Basin

High-resolution modelling systems have increasingly become an essential requirement to investigate ocean dynamics over a wide range of spatial and temporal scales, and to integrate the punctual ocean observations. When applied in coastal areas, they also have the potential to provide a detailed representation of transport and exchange processes at the sub-basin scale. This paper presents a validation exercise between the surface fields generated by the regional ocean modeling system (ROMS), developed for the Tyrrhenian Sea and downscaled for the Gulf of Naples (GNAM Gulf of Naples advanced model), and a 4 year-long (2009–2012) record of high-frequency radar (HFR) data. The comparison between hourly and seasonal model results and HFR surface fields is focused on the Gulf of Naples (GoN), where an observational network of three HFR sites has been operational since 2004, and on a specific subdomain characterized by the presence of the Sarno river, a long-term ecological research station (LTER-MC) and one important canyon area. An evaluation on a transect delimiting inshore–offshore zones in the GoN is also presented. The GNAM model was also compared with in situ hydrological parameters of temperatures and salinities retrieved at the LTER-MC fixed monitoring station. According to the skill metrics, basic circulation features are accurately reproduced by the circulation model, despite some model drawbacks in terms of increment of energy content in the surface current field occurring during specific seasonal events. The results allow us to identify potential model errors and to suggest useful improvements, the outcome also confirms the unique capability of HF radar systems to provide fine-scale measurements for the validation of numerical models and to counterbalance the lack of high-resolution measurements in coastal areas. © 2022 by the authors

Effect of COVID-19 pandemic on utilisation of community-based mental health care in North-East of Italy: A psychiatric case register study

Aims: WHO declared that mental health care should be considered one essential health service to be maintained during the coronavirus disease 2019 (COVID-19) pandemic. This study aims to describe the effect of lockdown and restrictions due to the COVID-19 pandemic in Italy on mental health services' utilisation, by considering psychiatric diagnoses and type of mental health contacts. Methods: The study was conducted in the Verona catchment area, located in the Veneto region (northeastern Italy). For each patient, mental health contacts were grouped into: (1) outpatient care, (2) social and supportive interventions, (3) rehabilitation interventions, (4) multi-professional assessments, (5) day care. A 'difference in differences' approach was used: difference in the number of contacts between 2019 and 2020 on the weeks of lockdown and intermediate restrictions was compared with the same difference in weeks of no or reduced restrictions, and such difference was interpreted as the effect of restrictions. Both a global regression on all contacts and separate regressions for each type of service were performed and Incidence Rate Ratios (IRRs) were calculated. Results: In 2020, a significant reduction in the number of patients who had mental health contacts was found, both overall and for most of the patients' characteristics considered (except for people aged 18-24 years for foreign-born population and for those with a diagnosis of schizophrenia. Moreover, in 2020 mental health contacts had a reduction of 57 096 (-33.9%) with respect to 2019; such difference remained significant across the various type of contacts considered, with rehabilitation interventions and day care showing the greatest reduction. Negative Binomial regressions displayed a statistically significant effect of lockdown, but not of intermediate restrictions, in terms of reduction in the number of contacts. The lockdown period was responsible of a 32.7% reduction (IRR 0.673; p-value <0.001) in the overall number of contacts. All type of mental health contacts showed a reduction ascribable to the lockdown, except social and supportive interventions. Conclusions: Despite the access to community mental health care during the pandemic was overall reduced, the mental health system in the Verona catchment area was able to maintain support for more vulnerable and severely ill patients, by providing continuity of care and day-by-day support through social and supportive interventions

Optical Monitoring of 3C 390.3 from 1995 to 2004 and Possible Periodicities in the Historical Light Curve

We report V, R, and I band CCD photometry of the radio galaxy 3C 390.3 obtained with the 1.56-m telescope of the Shanghai Astronomical Observatory from March 1995 to August 2004. Combining these data with data from the literature, we have constructed a historical light curve from 1894 to 2004 and searched for periodicities using the CLEANest program. We find possible periods of 8.30+-1.17, 5.37+-0.49, 3.51+-0.21, and 2.13+-0.08 years.Comment: Accepted by AJ, 34 pages, 11 figure

Protein kinase A negatively regulates Ca2+ signalling in Toxoplasma gondii

The phylum Apicomplexa comprises a group of obligate intracellular parasites that alternate between intracellular replicating stages and actively motile extracellular forms that move through tissue. Parasite cytosolic Ca2+ signalling activates motility, but how this is switched off after invasion is complete to allow for replication to begin is not understood. Here, we show that the cyclic adenosine monophosphate (cAMP)-dependent protein kinase A catalytic subunit 1 (PKAc1) of Toxoplasma is responsible for suppression of Ca2+ signalling upon host cell invasion. We demonstrate that PKAc1 is sequestered to the parasite periphery by dual acylation of PKA regulatory subunit 1 (PKAr1). Upon genetic depletion of PKAc1 we show that newly invaded parasites exit host cells shortly thereafter, in a perforin-like protein 1 (PLP-1)-dependent fashion. Furthermore, we demonstrate that loss of PKAc1 prevents rapid down-regulation of cytosolic [Ca2+] levels shortly after invasion. We also provide evidence that loss of PKAc1 sensitises parasites to cyclic GMP (cGMP)-induced Ca2+ signalling, thus demonstrating a functional link between cAMP and these other signalling modalities. Together, this work provides a new paradigm in understanding how Toxoplasma and related apicomplexan parasites regulate infectivity

Balmer Emission Line Profiles and the Complex Properties of Broad Line Regions in Active Galactic Nuclei

In this work we analyze a sample of AGN spectra, selected from the 6th Data Release of the Sloan Digital Sky Survey, exploiting a generalized technique of line profile analysis, designed to take into account the whole profiles of their broad emission lines. We find that the line profile broadening functions result from a complex structure, but we may be able to infer some constraints about the role of the geometrical factor, thus improving our ability to estimate AGN properties and their relation with the host galaxy. Our results suggest that flattening and inclination within the structure of the Broad Line Region (BLR) must be taken into account. We detect low inclinations of the BLR motion plane with respect to our line of sight, typically i < 20 degrees, with a geometrical effect which generally decreases as the line profile becomes broader.Comment: 13 pages, 11 figures, updated reference list. ApJ accepte

Protein kinase A negatively regulates Ca2+ signalling in Toxoplasma gondii.

The phylum Apicomplexa comprises a group of obligate intracellular parasites that alternate between intracellular replicating stages and actively motile extracellular forms that move through tissue. Parasite cytosolic Ca2+ signalling activates motility, but how this is switched off after invasion is complete to allow for replication to begin is not understood. Here, we show that the cyclic adenosine monophosphate (cAMP)-dependent protein kinase A catalytic subunit 1 (PKAc1) of Toxoplasma is responsible for suppression of Ca2+ signalling upon host cell invasion. We demonstrate that PKAc1 is sequestered to the parasite periphery by dual acylation of PKA regulatory subunit 1 (PKAr1). Upon genetic depletion of PKAc1 we show that newly invaded parasites exit host cells shortly thereafter, in a perforin-like protein 1 (PLP-1)-dependent fashion. Furthermore, we demonstrate that loss of PKAc1 prevents rapid down-regulation of cytosolic [Ca2+] levels shortly after invasion. We also provide evidence that loss of PKAc1 sensitises parasites to cyclic GMP (cGMP)-induced Ca2+ signalling, thus demonstrating a functional link between cAMP and these other signalling modalities. Together, this work provides a new paradigm in understanding how Toxoplasma and related apicomplexan parasites regulate infectivity