Long Term Aggresome Accumulation Leads to DNA Damage, p53-dependent Cell Cycle Arrest, and Steric Interference in Mitosis.

Juxtanuclear aggresomes form in cells when levels of aggregation-prone proteins exceed the capacity of the proteasome to degrade them. It is widely believed that aggresomes have a protective function, sequestering potentially damaging aggregates until these can be removed by autophagy. However, most in-cell studies have been carried out over a few days at most, and there is little information on the long term effects of aggresomes. To examine these long term effects, we created inducible, single-copy cell lines that expressed aggregation-prone polyglutamine proteins over several months. We present evidence that, as perinuclear aggresomes accumulate, they are associated with abnormal nuclear morphology and DNA double-strand breaks, resulting in cell cycle arrest via the phosphorylated p53 (Ser-15)-dependent pathway. Further analysis reveals that aggresomes can have a detrimental effect on mitosis by steric interference with chromosome alignment, centrosome positioning, and spindle formation. The incidence of apoptosis also increased in aggresome-containing cells. These severe defects developed gradually after juxtanuclear aggresome formation and were not associated with small cytoplasmic aggregates alone. Thus, our findings demonstrate that, in dividing cells, aggresomes are detrimental over the long term, rather than protective. This suggests a novel mechanism for polyglutamine-associated developmental and cell biological abnormalities, particularly those with early onset and non-neuronal pathologies

Discriminant analysis for lodging severity classification in wheat using RADARSAT-2 and Sentinel-1 data

Crop lodging - the bending of crop stems from their upright position or the failure of root-soil anchorage systems - is a major yield-reducing factor in wheat and causes deterioration of grain quality. The severity of lodging can be measured by a lodging score (LS)- an index calculated from the crop angle of inclination (CAI) and crop lodged area (LA). LS is difficult and time consuming to measure manually meaning that information on lodging occurrence and severity is limited and sparse. Remote sensing-based estimates of LS can provide more timely, synoptic and reliable information on crop lodging across vast areas. This information could improve estimates of crop yield losses, inform insurance loss adjusters and influence management decisions for subsequent seasons.This research - conducted in the 600 ha wheat sown area in the Bonifiche Ferraresi farm, located in Jolanda di Savoia, Ferrara, Italy - evaluated the performance of RADARSAT-2 and Sentinel-1 data to discriminate and classify lodging severity based on field measured LS. We measured temporal crop status characteristics related to lodging (e.g. lodged area, CAI, crop height) and collected relevant meteorological data (wind speed and rainfall) throughout May-June 2018. These field measurements were used to distinguish healthy (He) wheat from lodged wheat with different degrees of lodging severity (moderate, severe and very severe). We acquired multi-incidence angle (FQ8-27° and FQ21-41°) RADARSAT-2 and Sentinel-1 (40°) images and derived multiple metrics from them to discriminate and classify lodging severity. As a part of our data exploration, we performed a correlation analysis between the image-based metrics and LS. Next, a multi-temporal discriminant analysis approach, including a partial least squares (PLS-DA) method, was developed to classify lodging severities. We used the area under the curve-receiver operating characteristics (AUC-ROC) and confusion matrices to evaluate the accuracy of the PLS-DA classification models.Results show that (1) volume scattering components were highly correlated with LS at low incidence angles while double and surface scattering was more prevalent at high incidence angles; (2) lodging severity was best classified using low incidence angle R-FQ8 data (overall accuracy 72%) and (3) the Sentinel-1 data-based classification model was able to correctly identify 60% of the lodging severity cases in the study site. The results from this first study on classifying lodging severity using satellite-based SAR platforms suggests that SAR-based metrics can capture a substantial proportion of the observed variation in lodging severity, which is important in the context of operational crop lodging assessment in particular, and sustainable agriculture in general

The pandemic curvature of democratic space/time. A legal perspective.

The paper outlines if and how the pandemic contributed to the curvature of the democratic space/time. The entire legal toolbox was deployed and shaped to counter the pandemic's many impacts: not only regulatory strategies and decision-making processes, but also governance and even the legal lexicon. The short distance travelled in the journey towards recovery and resilience - which per se is a pandemic output - has already revealed the goal-oriented and performative face of the new NRRP/dedicated governance and regulatory framework, which seems able to temper the future and the risks it brings. It also paves the way to longer journeys, including the big transitions of our time - environmental and digital transitions, but also cultural and social ones - together with their transformative potential. Democracy is at a crossroad: it has the opportunity to be fit for the future (future-proof), or simply lost in transition(s). In this respect a few lessons emerge from the pandemic

Contenidos de fósforo total en suelos con características vérticas de la Provincia de Entre Ríos

p.53-58En Entre Ríos, los suelos con características vérticas ocupan el 69 por ciento del total de la superficie provincial, sin contar el D elta del río Paraná (Proyecto PN U D-FA O -INTA, 1980), generalmente estos suelos son deficientes en fósforo. El objetivo de este trabajo fue evaluar el contenido de fósforo total y su distribución en las fracciones orgánicas e inorgánicas en perfiles de suelos con características vérticas de Entre Ríos. Se tomaron muestras de suelo provenientes de cuatro Vertisoles, tres Alfisoles y un Molisol con características vérticas. En superficie los contenidos de fósforo total (Pt) de los suelos de los tres ordenes estudiados fueron de 200 a 300 mg kg. Del P total superficial de estos suelos predomina la fracción de P orgánico (Po) en relación a las formas inorgánicas (Pi). La distribución del P orgánico total en el perfil sigue un patrón general de disminución con el aumento de la profundidad, mientras que el Pi tiene una tendencia opuesta. Los horizontes C mostraron valores de P inorgánico de 150 a 200 mg de P kg `1 de suelo, reduciéndose aproximadamente a la mitad en los horizontes superficiales. Como resultado de este estudio, se puede concluir que los suelos del centro norte de la provincia de Entre Ríos presentan valores bajos de Pt. En estos suelos son im portantes las proporciones de Po en superficie, las cuales oscilan entre el 60 por ciento el 70 por ciento del Pt

Combining ASTER multispectral imagery analysis and support vector machines for rapid and cost-effective post-fire assessment: a case study from the Greek wildland fires of 2007

Remote sensing is increasingly being used as a cost-effective and practical solution for the rapid evaluation of impacts from wildland fires. The present study investigates the use of the support vector machine (SVM) classification method with multispectral data from the Advanced Spectral Emission and Reflection Radiometer (ASTER) for obtaining a rapid and cost effective post-fire assessment in a Mediterranean setting. A further objective is to perform a detailed intercomparison of available burnt area datasets for one of the most catastrophic forest fire events that occurred near the Greek capital during the summer of 2007. For this purpose, two ASTER scenes were acquired, one before and one closely after the fire episode. Cartography of the burnt area was obtained by classifying each multi-band ASTER image into a number of discrete classes using the SVM classifier supported by land use/cover information from the CORINE 2000 land nomenclature. Overall verification of the derived thematic maps based on the classification statistics yielded results with a mean overall accuracy of 94.6% and a mean Kappa coefficient of 0.93. In addition, the burnt area estimate derived from the post-fire ASTER image was found to have an average difference of 9.63% from those reported by other operationally-offered burnt area datasets available for the test region

Diabetic striatopathy: an updated overview of current knowledge and future perspectives

Purpose: Diabetic striatopathy (DS) is a rare complication of poorly controlled diabetes mellitus (DM), characterized by hyperglycemia associated with chorea/ballism and characteristic reversible basal ganglia abnormalities on computed tomography (CT) and/or magnetic resonance imaging (MRI). We propose a narrative review of the literature on this topic, currently unknown to most, and about which physicians should be aware. We intend to summarize, critically review, and take to mean the evidence on this disorder, describing its typical features. Methods: We searched Pubmed for English-language sources using the following keywords in the title and the abstract: diabetic striatopathy, hyperglycemic non-ketotic hemichorea/hemiballism, chorea/hemichorea associated with non-ketotic hyperglycemia, diabetic hemiballism/hemichorea, chorea, hyperglycemia, and basal ganglia syndrome. We collected scientific articles, including case reports, reviews, systematic reviews, and meta-analyses from the years 1975 to 2023. We eliminated duplicate, non-English language or non-related articles. Results: Older Asian women are more frequently affected. Suddenly or insidiously hemichorea/hemiballism, mainly in the limbs, and high blood glucose with elevated HbA1c in the absence of ketone bodies have been observed. Furthermore, CT striatal hyperdensity and T1-weighted MRI hyperintensity have been observed. DS is often a treatable disease following proper hydration and insulin administration. Histopathological findings are variable, and no comprehensive hypothesis explains the atypical cases reported. Conclusion: DS is a rare neurological manifestation of DM. If adequately treated, although treatment guidelines are lacking, the prognosis is good and life-threatening complications may occur occasionally. During chorea/hemiballism, we recommend blood glucose and HbA1c evaluation. Further studies are needed to understand the pathogenesis