Microarray-based analysis of fish egg quality after natural or controlled ovulation

BACKGROUND: The preservation of fish egg quality after ovulation-control protocols is a major issue for the development of specific biotechnological processes (e.g. nuclear transfer). Depending on the species, it is often necessary to control the timing of ovulation or induce the ovulatory process. The hormonal or photoperiodic control of ovulation can induce specific egg quality defects that have been thoroughly studied. In contrast, the impact on the egg transcriptome as a result of these manipulations has received far less attention. Furthermore, the relationship between the mRNA abundance of maternally-inherited mRNAs and the developmental potential of the egg has never benefited from genome-wide studies. Thus, the present study aimed at studying the rainbow trout (Oncorhynchus mykiss) egg transcriptome after natural or controlled ovulation using 9152-cDNA microarrays. RESULTS: The analysis of egg transcriptome after natural or controlled ovulation led to the identification of 26 genes. The expression patterns of 17 of those genes were monitored by real-time PCR. We observed that the control of ovulation by both hormonal induction and photoperiod manipulation induced significant changes in the egg mRNA abundance of specific genes. A dramatic increase of Apolipoprotein C1 (APOC1) and tyrosine protein kinase HCK was observed in the eggs when a hormonal induction of ovulation was performed. In addition, both microarray and real-time PCR analyses showed that prohibitin 2 (PHB2) egg mRNA abundance was negatively correlated with developmental success. CONCLUSION: First, we showed, for the first time in fish, that the control of ovulation using either a hormonal induction or a manipulated photoperiod can induce differences in the egg mRNA abundance of specific genes. While the impact of these modifications on subsequent embryonic development is unknown, our observations clearly show that the egg transcriptome is affected by an artificial induction of ovulation. Second, we showed that the egg mRNA abundance of prohibitin 2 was reflective of the developmental potential of the egg. Finally, the identity and ontology of identified genes provided significant hints that could result in a better understanding of the mechanisms associated with each type of ovulation control (i.e. hormonal, photoperiodic), and in the identification of conserved mechanisms triggering the loss of egg developmental potential

Le redistricting de 2011 à San Diego (Californie) : Mobilisation politique et représentativité de la communauté latino

Suite au recensement décennal de la population américaine, les districts électoraux sont redécoupés afin d’assurer le principe « d’un homme, une voix » tout en espérant offrir une meilleure représentation à l’ensemble des électeurs. Le cycle de redécoupage de 2011 concernait une multitude de législations sur l’ensemble du territoire américain. À San Diego en Californie, le redistricting des districts municipaux était conduit par une commission citoyenne indépendante qui avait pour but de faciliter la participation des citoyens à la prise de décision.Cet article propose de revenir sur le déroulement du processus à partir d’une étude de terrain. La communauté latino, devenue enjeu démographique et sociétal de taille, a massivement participé aux audiences publiques afin de faire valoir son appartenance territoriale et sa légitimité à obtenir une représentativité au conseil municipal. L’utilisation grandissante des Systèmes d’Informations Géographiques a constitué un élément nouveau dans l’implication des citoyens. Ils peuvent désormais manipuler leur espace de représentation politique et prendre conscience de l’impact des statistiques démographiques et politiques dans l’issue du redécoupage.Les mécanismes de mobilisations politiques seront appréhendés au travers de différentes cartes, d’extraits d’entretiens ou encore d’observations participantes, qui permettront in fine de conclure à une autre considération de l’espace politique, celle de la gouvernance locale.According to the decennial American Census, electoral districts are redrawn at each jurisdictional scale, through the principle of “one man, one vote”. In San Diego (California), the City Council redistricting has been conducted by a Commission of independent citizens. The main goal was to enhance the participation of citizens in the decision-making process.This article proposes to analyze this process from a fieldwork. The Latino community becomes a demographic and societal issue. This group has participated to public hearings in order to valorize their territorial belongings and legitimacy and to obtain a representativeness at the City Council.The recourse to Geographic Information Systems was a new piece of the citizens’ implication. They can now manipulate their representative political space and be aware of the demographic and political statistical impact of the process.All these political mobilizations mechanisms will be analyzed, thanks to maps, pieces of interview and participant observation. At the end, it is possible to conclude that a new consideration of the political space is emerging, the one of local governance

Methylome Response to Proteasome Inhibition by Pseudomonas syringae Virulence Factor Syringolin A

DNA methylation is an important epigenetic mark required for proper gene expression and silencing of transposable elements. DNA methylation patterns can be modified by environmental factors such as pathogen infection, in which modification of DNA methylation can be associated with plant resistance. To counter the plant defense pathways, pathogens produce effector molecules, several of which act as proteasome inhibitors. Here, we investigated the effect of proteasome inhibition by the bacterial virulence factor syringolin A (SylA) on genome-wide DNA methylation. We show that SylA treatment results in an increase of DNA methylation at centromeric and pericentromeric regions of Arabidopsis chromosomes. We identify several CHH differentially methylated regions (DMRs) that are enriched in the proximity of transcriptional start sites. SylA treatment does not result in significant changes in small RNA composition. However, significant changes in genome transcriptional activity can be observed, including a strong upregulation of resistance genes that are located on chromosomal arms. We hypothesize that DNA methylation changes could be linked to the upregulation of some atypical members of the de novo DNA methylation pathway, namely AGO3, AGO9, and DRM1. Our data suggests that modification of genome-wide DNA methylation resulting from an inhibition of the proteasome by bacterial effectors could be part of an epi-genomic arms race against pathogens. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license

Post-ovulatory ageing and egg quality: A proteomic analysis of rainbow trout coelomic fluid

BACKGROUND: In fish, oocyte post-ovulatory ageing is associated with egg quality decrease. During this period, eggs are held in the body cavity where they bath in a semi-viscous liquid known as coelomic fluid (CF). CF components are suspected to play a role in maintaining oocyte fertility and developmental competence (egg quality). However, CF proteic composition remains poorly studied. Thus rainbow trout CF proteome was studied during the egg quality decrease associated with oocyte post-ovulatory ageing. METHODS: High resolution two-dimensional gel electrophoresis was used to analyze the proteome of rainbow trout (Oncorhynchus mykiss) CF in relationship with the egg quality decrease associated with oocyte post-ovulatory ageing. A first experiment was performed using CF pools originating from 17 females sampled at ovulation as well as 7, 14 and 21 days later. These observations were verified using a second set of CF pools originating from 22 females sampled 5 and 16 days following ovulation. RESULTS: Approximately 200 protein spots of 10–105 kDa molecular mass and 3–10 pI were detected in CF samples. Several protein spots, while undetected at the time of ovulation, exhibited a progressive and strong accumulation in CF during post-ovulatory ageing. After silver-staining and Matrix-Assisted Laser Desorption Time Of Flight (MALDI-TOF) mass spectrometer analysis, some of these protein spots were identified as lipovitellin II fragments. CONCLUSIONS: These observations suggest that egg protein fragments accumulate in the CF during the post-ovulatory period and could therefore be used to detect egg quality defects associated with oocyte post-ovulatory ageing

Rayonnement sonore dans un écoulement subsonique complexe en régime harmonique (analyse et simulation numérique du couplage entre les phénomènes acoustiques et hydrodynamiques)

La thèse porte sur la simulation, en régime fréquentiel, du rayonnement acoustique en écoulement subsonique quelconque et dans un domaine infini. L'approche choisie s'appuie sur la résolution d'un système équivalent aux équations d'Euler linéarisées : le modèle de Galbrun. Ce modèle repose sur une représentation mixte Lagrange-Euler et aboutit à une équation dont l'unique inconnue est la perturbation du déplacement Lagrangien. Une des difficultés de l'approche de Galbrun est qu'une discrétisation directe de cette équation par une méthode d'éléments finis standard n'est pas stable. Un moyen de contourner cet obstacle est d'écrire une équation augmentée en ajoutant une nouvelle inconnue, le rotationnel du déplacement, appelée par abus vorticité. Cette approche conduit à un système qui couple une équation de type équation des ondes avec une équation de transport en régime fréquentiel. Et elle permet l'utilisation de couches parfaitement adaptées (PML) pour borner le domaine de calcul. La première partie du manuscrit est dédiée à l étude de l équation de transport harmonique et de sa résolution numérique, en particulier par un schéma de type Galerkin discontinu. Un des points délicats est lié au caractère oscillant des solutions de l'équation. Une fois cette étape franchie, la résolution du problème de propagation acoustique a été abordée. Une approximation basée sur l'utilisation d'éléments finis mixtes continus-discontinus avec couches parfaitement adaptées (PML) a été étudiée. En particulier, les caractères bien posés des problèmes continu et discret ainsi que la convergence du schéma numérique ont été démontrés sous certaines conditions sur l'écoulement porteur. Enfin, une mise en œuvre a été effectuée. Les résultats montrent la validité de cette approche mais aussi sa pertinence dans le cas d'écoulements complexes, voire d'écoulements dits instablesThis thesis deals with the numerical simulation of time harmonic acoustic propagation in an arbitrary mean flow in an unbounded domain. Our approach is based on an equation equivalent to the linearized Euler equations called the Galbrun equation. It is derived from a mixed Eulerian-Lagrangian formulation and results in a single equation whose only unknown is the perturbation of the Lagrangian displacement. A direct solution using finite elements is unstable but this difficulty can be overcome by using an augmented equation which is constructed by adding a new unknown, the vorticity, defined as the curl of the displacement. This leads to a set of equations coupling a wave like equation with a time harmonic transport equation which allows the use of perfectly matched layers (PML) at artificial boundaries to bound the computational domain. The first part of the thesis is a study of the time harmonic transport equation and its approximation by means of a discontinuous Galerkin scheme, the difficulties coming from the oscillating behaviour of its solutions. Once these difficulties have been overcome, it is possible to deal with the resolution of the acoustic propagation problem. The approximation method is based on a mixed continuous-Galerkin and discontinuous-Galerkin finite element scheme. The well-posedness of both the continuous and discrete problems is established and the convergence of the approximation under some mean flow conditions is proved. Finally a numerical implementation is achieved and numerical results are given which confirm the validity of the method and also show that it is relevant in complex cases, even for unstable flowsTOULOUSE-INSA-Bib. electronique (315559905) / SudocSudocFranceF