Caracterização in vitro da modificação pós-traducional das proteínas GInB e GInK de Herbaspirillum seropedicae /

Orientadora : Elaine Machado BenelliCo-orientador : Emanuel Maltempi de SouzaTese (doutorado) - Universidade Federal do Paraná, Setor de Ciências Biológicas, Programa de Pós-Graduação em Bioquímica. Defesa: Curitiba, 2007Inclui bibliografi

Clonagem, superexpressao e purificaçao de uma proteína mutante de Herbaspirillum seropedicae

Orientador: Emanuel Maltempi de Souza e Elaine Machado BenelliMonografia (Bacharelado) - Universidade Federal do Paraná. Setor de Ciencias Biológicas. Curso de Graduaçao em Ciencias BiológicasResumo : Herbaspirillum seropedicae é um diazotrofo endofítico que foi inicialmente isolado de gramíneas. Neste organismo, a fixação de nitrogênio é regulada positivamente pela proteína NifA. A proteína PII, produto do gene g/nB, possui um papel central nesta regulação. Dados de difração de raios-X mostraram que a proteína PII de H. seropedicae apresenta um motivo estrutural em hélice 310 a partir do resíduo Gly 108, na região C-terminal, que está ausente na proteína PII de E. colí, porém presente na proteína GlnK (paráloga à PII). De fato, a proteína PII de H. seropedicae parece ser funcionalmente semelhante a proteína GlnK de E. co/i. Enquanto a proteína GlnK de E. calí controla indiretamente a atividade de NifA, aliviando a inibição de NifL sobre NifA em condições de desrepressão, a PII de H. seropedicae parece controlar diretamente a atividade de NifA. Estes dados sugerem que a região C-terminal da proteína PII de H. seropedicae pode estar envolvida no controle da atividade de NifA. Para avaliar a importância funcional da região C-terminal da proteína PII de H. seropedicae foram construídos dois mutantes do gene g/nB. Em um dos mutantes, obtido por mutagênese sítio dirigida, os resíduos Gly108 e Pro109 foram substituídos por resíduos de alanina e esta proteína foi denominada PII-AA. O outro gene mutante, obtido por mutagênese aleatória, apresenta quatro substituições de resíduos: Gln3-Arg; Thr5- Ala; Gly87-Cys; Gly108-Trp e codifica para a proteína PII-AL. Os genes g/nB mutantes foram clonados no vetor de expressão pET28b+, originando os plasmídeos pACB3 e pACB51, respectivamente. O sequenciamento destes genes mostrou que o mutante aleatório apresenta a deleção de um nucleotídeo que torna o gene não funcional. A proteína PII-AA foi superexpressa a partir do plasmídeo pACB3 e purificada por cromatografia de troca iônica e de afinidade

Data release 2 of S-PLUS : accurate template-fitting based photometry covering ∼1000 deg2 in 12 optical filters

The Southern Photometric Local Universe Survey (S-PLUS) is an ongoing survey of ∼9300 deg2 in the southern sky in a 12-band photometric system. This paper presents the second data release (DR2) of S-PLUS, consisting of 514 tiles covering an area of 950 deg2. The data has been fully calibrated using a new photometric calibration technique suitable for the new generation of wide-field multifilter surveys. This technique consists of a χ2 minimization to fit synthetic stellar templates to already calibrated data from other surveys, eliminating the need for standard stars and reducing the survey duration by ∼15 per cent. We compare the template-predicted and S-PLUS instrumental magnitudesto derive the photometric zero-points(ZPs). We show that these ZPs can be further refined by fitting the stellar templates to the 12 S-PLUS magnitudes, which better constrain the models by adding the narrow-band information. We use the STRIPE82 region to estimate ZP errors, which are 10 mmags for filters J0410, J0430, g,J0515, r,J0660, i,J0861 and z; 15 mmags for filterJ0378; and 25 mmagsfor filters u and J0395. We describe the complete data flow of the S-PLUS/DR2 from observations to the final catalogues and present a brief characterization of the data. We show that, for a minimum signal-to-noise threshold of 5, the photometric depths of the DR2 range from 19.1 to 20.5 mag (measured in Petrosian apertures), depending on the filter. The S-PLUS DR2 can be accessed from the website: https://splus.cloud

Modulation of gene expression in mice treated with sugar and whey protein isolate / Modulação da expressão gênica em camundongos tratados com açúcar e isolado de proteína de soro de leite

This study compared the effects of three different types of diets (regular diet, high in sugar diet and high in sugar plus whey protein isolate supplementation diet) in mice. After 3 months, gene expression in liver and in adipose tissue was measured. We found that diets influenced in gene expression, but not supplementation. However, supplementation showed an anorectic effect, and protected animals from hepatic steatosis. In addition, the diets did not influence the myeloperoxidase activity in the adipose tissue, showing little influence on the infiltration of white cells in this tissue

The Southern Photometric Local Universe Survey (S-PLUS) : improved SEDs, morphologies, and redshifts with 12 optical filters

The Southern Photometric Local Universe Survey (S-PLUS) is imaging~300 deg2 of the celestial sphere in 12 optical bands using a dedicated 0.8 m robotic telescope, the T80-South, at the Cerro Tololo Inter-american Observatory, Chile. The telescope is equipped with a 9.2k×9.2k e2v detector with 10μm pixels, resulting in a field of view of 2 deg2 with a platescale of 0.55 arcsec pixel−1. The survey consists of four main subfields, which include twonon-contiguous fields at high Galactic latitudes (|b|>30◦, 8000 deg2) and two areas of the Galactic Disc and Bulge (for an additional 1300 deg2). S-PLUS uses the Javalambre 12-band magnitude system, which includes the 5ugrizbroad-band filters and 7 narrow-band filters centred on prominent stellar spectral features: the Balmer jump/[OII], Ca H+K, Hδ, Gband, Mg b triplet, Hα, and the Ca triplet. S-PLUS delivers accurate photometric redshifts (δz/(1+z)=0.02 or better) for galaxies withr<19.7 AB mag andz<0.4, thus producing a 3D map of the local Universe over a volume of more than 1 (Gpc/h)3. The final S-PLUS catalogue will also enable the study of star formation and stellar populations in and around the MilkyWay and nearby galaxies, as well as searches for quasars, variable sources, and low-metallicity stars. In this paper we introduce the main characteristics of the survey, illustrated with science verification data highlighting the unique capabilities of S-PLUS. We also present the first public data release of ~336 deg2 of the Stripe 82 area, in 12 bands, to a limiting magnitude ofr=21, available atdatalab.noao.edu/splus

J-PLUS: A wide-field multi-band study of the M15 globular cluster. Evidence of multiple stellar populations in the RGB

The Javalambre Photometric Local Universe Survey (J-PLUS) provides wide field-of-view images in 12 narrow, intermediate and broad-band filters optimized for stellar photometry. Here we have applied J-PLUS data for the first time for the study of Galactic GCs using science verification data obtained for the very metal-poor GC M\,15. Our J-PLUS data provide low-resolution spectral energy distributions covering the near-UV to the near-IR, allowing us to search for MPs based on pseudo-spectral fitting diagnostics. J-PLUS CMDs are found to be particularly useful to search for splits in the sequences formed by the upper red giant branch (RGB) and asymptotic giant branch (AGB) stars. We interpret these split sequences as evidence for the presence of MPs. This demonstrates that the J-PLUS survey will have sufficient spatial coverage and spectral resolution to perform a large statistical study of GCs through multi-band photometry in the coming years.Comment: 11 pages, 11 figures. Accepted for publication @ A&

S-PLUS : exploring wide field properties of multiple populations in galactic globular clusters at different metallicities

Multiple stellar populations (MSPs) are a ubiquitous phenomenon in Galactic globular clusters (GCs). By probing different spectral ranges affected by different absorption lines using the multiband photometric survey S-PLUS, we study four GCs – NGC 104, NGC 288, NGC 3201, and NGC 7089 – that span a wide range of metallicities. With the combination of broad and narrow-band photometry in 12 different filters from 3485A (u) to 9114A (z), we identified MSPs along the rectified red-giant branch in colour–magnitude diagrams and separated them using a K-means clustering algorithm. Additionally, we take advantage of the large Field of View of the S-PLUS detector to investigate radial trends in our sample. We report on six colour combinations that can be used to successfully identify two stellar populations in all studied clusters and show that they can be characterized as Na-rich and Na-poor. For both NGC 288 and NGC 7089, their radial profiles show a clear concentration of 2P population. This directly supports the formation theories that propose an enrichment of the intra-cluster medium and subsequent star formation in the more dense central regions. However, in the case of NGC 3201, the trend is reversed. The 1P is more centrally concentrated, in direct contradiction with previous literature studies. NGC 104 shows a well-mixed population. We also constructed radial profiles up to 1 half-light radius of the clusters with HST data to highlight that radial differences are lost in the inner regions of the GCs and that wide-field studies are essential when studying this

Caracterização in vitro da modificação pós-traducional das proteínas GInB e GInK de Herbaspirillum seropedicae /

Orientadora : Elaine Machado BenelliCo-orientador : Emanuel Maltempi de SouzaTese (doutorado) - Universidade Federal do Paraná, Setor de Ciências Biológicas, Programa de Pós-Graduação em Bioquímica. Defesa: Curitiba, 2007Inclui bibliografi

Polimorfismos de marcadores ALU em indivíduos de povos nativos americanos e siberianos

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Polimorfismos de marcadores ALU em indivíduos de povos nativos americanos e siberianos

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