Circulating miR-320b and miR-483-5p levels are associated with COVID-19 in-hospital mortality

none28noThe stratification of mortality risk in COVID-19 patients remains extremely challenging for physicians, especially in older patients. Innovative minimally invasive molecular biomarkers are needed to improve the prediction of mortality risk and better customize patient management. In this study, aimed at identifying circulating miRNAs associated with the risk of COVID-19 in-hospital mortality, we analyzed serum samples of 12 COVID-19 patients by small RNA-seq and validated the findings in an independent cohort of 116 COVID-19 patients by qRT-PCR. Thirty-four significantly deregulated miRNAs, 25 downregulated and 9 upregulated in deceased COVID-19 patients compared to survivors, were identified in the discovery cohort. Based on the highest fold-changes and on the highest expression levels, 5 of these 34 miRNAs were selected for the analysis in the validation cohort. MiR-320b and miR-483-5p were confirmed to be significantly hyper-expressed in deceased patients compared to survived ones. Kaplan-Meier and Cox regression models, adjusted for relevant confounders, confirmed that patients with the 20% highest miR-320b and miR-483-5p serum levels had three-fold increased risk to die during in-hospital stay for COVID-19. In conclusion, high levels of circulating miR-320b and miR-483-5p can be useful as minimally invasive biomarkers to stratify older COVID-19 patients with an increased risk of in-hospital mortality.restrictedGiuliani, Angelica; Matacchione, Giulia; Ramini, Deborah; Di Rosa, Mirko; Bonfigli, Anna Rita; Sabbatinelli, Jacopo; Monsurrò, Vladia; Recchioni, Rina; Marcheselli, Fiorella; Marchegiani, Francesca; Piacenza, Francesco; Cardelli, Maurizio; Galeazzi, Roberta; Pomponio, Giovanni; Ferrarini, Alessia; Gabrielli, Armando; Baroni, Silvia Svegliati; Moretti, Marco; Sarzani, Riccardo; Giordano, Piero; Cherubini, Antonio; Corsonello, Andrea; Antonicelli, Roberto; Procopio, Antonio Domenico; Ferracin, Manuela; Bonafè, Massimiliano; Lattanzio, Fabrizia; Olivieri, FabiolaGiuliani, Angelica; Matacchione, Giulia; Ramini, Deborah; Di Rosa, Mirko; Bonfigli, Anna Rita; Sabbatinelli, Jacopo; Monsurrò, Vladia; Recchioni, Rina; Marcheselli, Fiorella; Marchegiani, Francesca; Piacenza, Francesco; Cardelli, Maurizio; Galeazzi, Roberta; Pomponio, Giovanni; Ferrarini, Alessia; Gabrielli, Armando; Baroni, Silvia Svegliati; Moretti, Marco; Sarzani, Riccardo; Giordano, Piero; Cherubini, Antonio; Corsonello, Andrea; Antonicelli, Roberto; Procopio, Antonio Domenico; Ferracin, Manuela; Bonafè, Massimiliano; Lattanzio, Fabrizia; Olivieri, Fabiol

A polymorphic variant of the insulin-like growth factor 1 (IGF-1) receptor correlates with male longevity in the Italian population: a genetic study and evaluation of circulating IGF-1 from the "Treviso Longeva (TRELONG)" study

<p>Abstract</p> <p>Background</p> <p>An attenuation of the insulin-like growth factor 1 (IGF-1) signaling has been associated with elongation of the lifespan in simple metazoan organisms and in rodents. In humans, IGF-1 level has an age-related modulation with a lower concentration in the elderly, depending on hormonal and genetic factors affecting the IGF-1 receptor gene (<it>IGF-1R</it>).</p> <p>Methods</p> <p>In an elderly population from North-eastern Italy (<it>n </it>= 668 subjects, age range 70–106 years) we investigated the <it>IGF-1R </it>polymorphism G3174A (<it>rs2229765</it>) and the plasma concentration of free IGF-1. Frequency distributions were compared using χ²-test "Goodness of Fit" test, and means were compared by one-way analysis of variance (ANOVA); multiple regression analysis was performed using JMP7 for SAS software (SAS Institute, USA). The limit of significance for genetic and biochemical comparison was set at α = 0.05.</p> <p>Results</p> <p>Males showed an age-related increase in the A-allele of <it>rs2229765 </it>and a change in the plasma level of IGF-1, which dropped significantly after 85 years of age (85+ group). In the male 85+ group, A/A homozygous subjects had the lowest plasma IGF-1 level. We found no clear correlation between <it>rs2229765 </it>genotype and IGF-1 in the females.</p> <p>Conclusion</p> <p>These findings confirm the importance of the <it>rs2229765 </it>minor allele as a genetic predisposing factor for longevity in Italy where a sex-specific pattern for IGF-1 attenuation with ageing was found.</p

jobonaf/calicantus: calicantus 1.0

Calicantus is a service developed to collect and visualize air quality data. Through an interactive web interface, you can explore and analyze the data. Interactive maps, cluster analysis and other tools facilitate the visualization and help the interpretation of past pollution episodes and of the current situation. Furthermore, air quality forecasts for today and three days ahead are shown, provided by seven chemistry-transport models of the Copernicus Atmosphere Monitoring Service. More info: https://shiny.arpae.it/calicantus-intro

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air quality model benchmarkin

A proposal to overcome the ambiguity of the aerosol and communicate it more easily, with the help of Captain Sankey

In order to give a simple and straight answer to the question “who is to blame for air pollution?”, a screening approach is proposed, expressing the emission of each anthropogenic precursor in terms of “PM10 equivalent”. The anthropogenic aerosol formation factor (AFs) is estimated, starting from de Leeuw (2002) and Johansson et al. (2003) and introducing corrections based on chemistry-transport model simulations, in order to better express the local dynamics of the Po Valley

A dashboard for near-real-time air pollution episodes analysis and support to decision making

In this work, the web platform <i>calicantus </i>is described, a service developed to collect and visualize air quality data. Most of the Italian regional environmental agencies take part in this project and share air quality observed data, every day. Through an interactive web interface, the registered user can explore and analyse the data. Interactive maps, cluster analysis, time series analysis and other tools facilitate the visualization and help the interpretation of past pollution episodes and of the current situation. Furthermore, air quality forecasts for today and three days ahead - provided by the Copernicus Atmosphere Monitoring Service - are shown

Profili di vento di GAP. Diagnostica e correzione a posteriori

GAP è uno dei moduli della catena WRF-GAP-SURFPRO-FARM presso il Centro Regionale di Modellistica Ambientale di ARPA-FVG. Qui sono presentati i risultati della diagnostica e di una possibile correzione a posteriori dei profili di vento di GAP.<br

A hybrid Eulerian-Lagrangian-statistical approach to evaluate air quality in a mixed residential-industrial environment

Aim of this work is to evaluate the air quality in the mixed urban-industrial environment of the city of Trieste, Italy. In particular, PM10 daily exceedances have to be estimated in a residential district close to a large iron plant. Due to the complex orographic context, to the land-sea discontinuity and to the local scale wind regimes, concentration gradients can be often strong. Therefore it can be misleading to rely only on the monitoring network to assess air quality, since some stations could often have a limited spatial representativeness. On the other hand, under some conditions not only local emissions, but also long-range transport of PM10 or gaseous precursors from the Po Valley cannot be neglected. <br>To take into account all these elements, we used an Eulerian chemistry-transport model (CTM) to simulate the daily regional background concentrations and a Lagrangian model (LM) to simulate the local impact of the emissions of the iron plant (blast furnace, coking plant and casthouse). Regional scale background concentrations of PM10 have been estimated with a data fusion approach, interpolating the observations of the background stations using concentrations simulated by the CTM as proxy. Then the residuals over the urban stations have been interpolated using concentrations simulated by the LM as proxy. Finally, the sum of background and residuals is compared daily with the 50 µg/m3 threshold, in order to assess air quality with a detail suitable for epidemiological purposes.<br>The effectiveness of different strategies for impact mitigation is also assessed.<br><br

Sviluppo di algoritmi di controllo di qualità automatico per i dati della rete di monitoraggio della qualità dell'aria

Sviluppo di algoritmi di controllo di qualità automatico per i dati della rete di monitoraggio della qualità dell'ari

PROGETTO SUPERSITO

The project is focused on a detailed study of some chemical, physical and toxicological parameters and on health, epidemiological and environmental assessment by interpretative models, in the atmosphere of Emilia-Romagna (Italy). The project rises from the necessity to improve  knowledge about environmental and health aspects of fine and ultrafine particles, in primary and secondary components, in the atmosphere. The project, structured in 7 workpackages, is organized in two measurement programmes: the routine one that has a mainly daily time resolution, and the intensive one with high time resolution and a higher chemical speciation than the routine one. The sampling sites are five: three in urban areas (Bologna, Parma and Rimini), one in a rural area (San Pietro Capofiume) and one in a remote area (Monte Cimone). Parallel to outdoor studies, a workpackage  is planned for indoor studies and chemical composition analysis with the  outdoor/indoor ratio for characterizing indoor human exposure to outdoor pollution.Le projet consiste en une étude détaillée des paramètres chimiques, physiques et météorologiques et une évaluation sanitaire, épidémiologique et l’environnementale (grâce à des modèles), dans l’atmosphère de la région Emilie-Romagne. L’objectif du projet est le progrès des connaissances sur les aspects environnementaux et sanitaires des particules fines et ultra-fines en composants primaires et/ou secondaires dans l’ atmosphère. Le projet, articulé en 7 lignes directrices,  est organisé en deux programmes de mesures: un programme de routine avec une fréquence temporelle surtout quotidienne et un programme intensif avec une fréquence temporelle èlevée et un éventail plus large de l'analyse.  Les sites de mesure sont au nombre de cinq:  trois dans la zone urbaine (Bologne, Parme et Rimini), un dans la zone rurale (San Pietro Capofiume) et un dans une région reculée (Monte Cimone). Parallèlement, une ligne directrice se concentre sur l’étude du rapport intérieur/extérieur des composants  de la qualité de l’air dans les bâtiments, pour caractériser l'exposition humaine à la pollution extérieure lorsque les sujets son à l’intérieur.Lo scopo del progetto è la realizzazione di uno studio integrato dell’inquinamento dell’atmosfera in Emilia-Romagna attraverso misure di parametri chimici, fisici, tossicologici e valutazioni sanitarie, epidemiologiche ed ambientali mediante modelli interpretativi. Il progetto nasce dalla necessità di migliorare le conoscenze relativamente agli aspetti ambientali e sanitari del particolato fine ed ultrafine, nelle componenti e/o secondarie, presente in atmosfera. Il progetto, strutturato in 7 linee progettuali, è organizzato in due programmi di misure: uno routinario in cui sono previsti campionamenti giornalieri ed uno intensivo in cui è prevista una speciazione più dettagliata con maggiore risoluzione temporale. I siti di campionamento sono 5: 3 in area urbana (Bologna, Parma e Rimini), 1 in  area rurale (San Pietro Capofiume) e 1 in area remota (Monte Cimone). Contestualmente agli studi outdoor è prevista una linea progettuale che si occupa dello studio del particolato, della sua composizione chimica nel rapporto qualità dell’aria outdoor/indoor e della caratterizzazione dell’esposizione della popolazione in ambienti indoor agli inquinanti tipici dell’ambiente outdoor