Estrous behavior in dairy cows: identification of underlying mechanisms and gene functions

Selection in dairy cattle for a higher milk yield has coincided with declined fertility. One of the factors is reduced expression of estrous behavior. Changes in systems that regulate the estrous behavior could be manifested by altered gene expression. This literature review describes the current knowledge on mechanisms and genes involved in the regulation of estrous behavior. The endocrinological regulation of the estrous cycle in dairy cows is well described. Estradiol (E2) is assumed to be the key regulator that synchronizes endocrine and behavioral events. Other pivotal hormones are, for example, progesterone, gonadotropin releasing hormone and insulin-like growth factor-1. Interactions between the latter and E2 may play a role in the unfavorable effects of milk yield-related metabolic stress on fertility in high milk-producing dairy cows. However, a clear understanding of how endocrine mechanisms are tied to estrous behavior in cows is only starting to emerge. Recent studies on gene expression and signaling pathways in rodents and other animals contribute to our understanding of genes and mechanisms involved in estrous behavior. Studies in rodents, for example, show that estrogen-induced gene expression in specific brain areas such as the hypothalamus play an important role. Through these estrogen-induced gene expressions, E2 alters the functioning of neuronal networks that underlie estrous behavior, by affecting dendritic connections between cells, receptor populations and neurotransmitter releases. To improve the understanding of complex biological networks, like estrus regulation, and to deal with the increasing amount of genomic information that becomes available, mathematical models can be helpful. Systems biology combines physiological and genomic data with mathematical modeling. Possible applications of systems biology approaches in the field of female fertility and estrous behavior are discusse

Minder verliezen door betere benutting : bemesting 'Koeien & Kansen', 1999-2001

Dit rapport behandelt het optimaliseren van de bemesting binnen de randvoorwaarden van MINAS, in het kader van het project ‘Koeien & Kansen’. Hierbij is het opstellen van een jaarplan voor de bemesting essentieel. In dit jaarplan is de hoeveelheid kunstmeststikstof, die per ha cultuurgrond aangevoerd mag worden, het uitgangspunt. Deze kunstmest en de op het bedrijf beschikbare mest worden toegedeeld aan de verschillende gewassen. Het belangrijkste doel van het jaarplan voor de N-bemesting is het berekenen van de Njaargift op het intensief gebruikte grasland, waarbij de MINAS-eindnorm voor N kan worden gehaal

Toevoegen van nitrificatieremmer aan dunne rundmest op grasland, 2003 : literatuurstudie en oriënterend veldonderzoek

In het voorjaar is de periode tussen het toedienen van de meststoffen en de oogst van een snede gras vrij lang. In deze periode is de hoeveelheid neerslag vaak groter dan de vochtonttrekking door het gewas en de verdamping. De stikstof (N) kan dan verloren gaan door uitspoeling of denitrificatie. Onderzoek in het verleden heeft aangetoond dat het gebruik van voorjaarsmeststoffen als ammoniumsulfaatsalpeter (ASS) en zwavelzure ammoniak (ZA) een positief effect heeft op de opbrengst en de N-benutting. Voorjaarsmeststoffen bevatten meer N in de vorm van ammonium dan van nitraat. Door het toevoegen van een nitrificatieremmer aan de kunstmest wordt dit positieve effect nog vergroot. Een voorbeeld van een nitrificatieremmer is dimethylpyrazolfosfaat (DMPP). Entec26 is ASS waaraan deze nitrificatieremmer is toegevoegd. Dit rapport bevat een literatuurstudie over het gebruik van DMPP als nitrificatieremmer en geeft de resultaten van een praktijktoets in 2003 op drie 'Koeien & Kansen'-bedrijven, waar bij het uitrijden van de dunne rundermest DMPP aan die mest is toegevoeg

Holographic model for dilepton production in p-p collisions

We propose a holographic model for dilepton production in proton-proton collisions based on the exchange of vector mesons. The holographic hard wall model is used to describe the dynamics and interactions of vector mesons and baryons. We estimate the parameters lambda, mu, nu that characterize the angular distribution of the produced dileptons in a region of q_T^2 << Q^2, where perturbative QCD presents an effective strong coupling due to large logarithm corrections.Comment: Revision: V4 In this version we modified the dimension of the fermionic operator to match the canonical dimension of a baryonic operator. This modification affected slightly our results with respect to v3. 25 pages, 3 Latex figures + 6 eps figures, 5 tables. To appear in Nucl. Phys.

The Position of High Frequency Waves with Respect to the Granulation Pattern

High frequency velocity oscillations were observed in the spectral lines Fe I 543.45nm and 543.29nm, using 2D spectroscopy with a Fabry- Perot and speckle reconstruction, at the VTT in Tenerife. We investigate the radial component of waves with frequencies in the range 8 - 22mHz in the internetwork, network and a pore. We find that the occurrence of waves do not show any preference on location and are equally distributed over down-flows and up-flows, regardless of the activity of the observed area in the line of Fe I 543.45nm. The waves observed in the lower formed line of Fe I 543.29nm seem to appear preferentially over down-flows.Comment: Article has 12 pages and 7 images. It is accepted in Solar Physics Journa

Molecular characterization and phylogeny of Shiga toxin–producing Escherichia coli isolates obtained from two Dutch regions using whole genome sequencing

AbstractShiga toxin–producing Escherichia coli (STEC) is one of the major causes of human gastrointestinal disease and has been implicated in sporadic cases and outbreaks of diarrhoea, haemorrhagic colitis and haemolytic uremic syndrome worldwide. In this study, we determined the molecular characteristics and phylogenetic relationship of STEC isolates, and their genetic diversity was compared to that of other E. coli populations. Whole genome sequencing was performed on 132 clinical STEC isolates obtained from the faeces of 129 Dutch patients with gastrointestinal complaints. STEC isolates of this study belonged to 44 different sequence types (STs), 42 serogenotypes and 14 stx subtype combinations. Antibiotic resistance genes were more frequently present in stx1-positive isolates compared to stx2 and stx1 + stx2–positive isolates. The iha, mchB, mchC, mchF, subA, ireA, senB, saa and sigA genes were significantly more frequently present in eae-negative than in eae-positive STEC isolates. Presence of virulence genes encoding type III secretion proteins and adhesins was associated with isolates obtained from patients with bloody diarrhoea. Core genome phylogenetic analysis showed that isolates clustered according to their ST or serogenotypes irrespective of stx subtypes. Isolates obtained from patients with bloody diarrhoea were from diverse phylogenetic backgrounds. Some STEC isolates shared common ancestors with non-STEC isolates. Whole genome sequencing is a powerful tool for clinical microbiology, allowing high-resolution molecular typing, population structure analysis and detailed molecular characterization of strains. STEC isolates of a substantial genetic diversity and of distinct phylogenetic groups were observed in this study

BRAF mutation-specific promoter methylation of FOX genes in colorectal cancer

Background: Cancer-specific hypermethylation of (promoter) CpG islands is common during the tumorigenesis of colon cancer. Although associations between certain genetic aberrations, such as BRAF mutation and microsatellite instability, and the CpG island methylator phenotype (CIMP), have been found, the mechanisms by which these associations are established are still unclear. We studied genome-wide DNA methylation differences between

Pathologic features of prostate cancer found at population-based screening with a four-year interval

BACKGROUND: The currently recommended frequency for prostate-specific antigen (PSA) screening tests for prostate cancer is 1 year, but the optimal screening interval is not known. Our goal was to determine if a longer interval would compromise the detection of curable prostate cancer. METHODS: A cohort of 4491 men aged 55-75 years, all participants in the Rotterdam section of the European Randomized Study of (population-based) Screening for Prostate Cancer, were invited to participate in an initial PSA screening. Men who received that screening were invited for a second screen 4 years later. Pathology findings from needle biopsy cores were compared for men in both rounds. Statistical tests were two-sided. RESULTS: A total of 4133 men were screened in the first round (the prevalence screen), and 2385 were screened in the second round. The median amount of cancer in needle biopsy sets was 7.0 mm (95% confidence interval [CI] = 5.4 mm to 8.6 mm) in the first round and 4.1 mm (95% CI = 2.6 mm to 5.6 mm) in the second round (P =.001). Thirty-six percent of the adenocarcinomas detected in the first round but only 16% of those detected in the second round had a Gleason score of 7 or higher (mean difference = 20% [95% CI = 10% to 30%]; P<.001). Whereas 25% of the adenocarcinomas detected in the first round had adverse prognostic features, only 6% of those detected in the second round did (mean difference = 19% [95% CI = 11% to 26%]; P<.001). Baseline PSA values were predictive for the amount of tumor in biopsies in men with cancer in the first round but not for that in the second round. CONCLUSION: Most large prostate cancers with high serum PSA levels were effectively detected in a prevalence screen. In this population, a screening interval of 4 years appears to be short enough to constrain the development of large tumors, although it is inconclusive whether this will result in a survival benefit

Accessing transversity with interference fragmentation functions

We discuss in detail the option to access the transversity distribution function $h_1(x)$ by utilizing the analyzing power of interference fragmentation functions in two-pion production inside the same current jet. The transverse polarization of the fragmenting quark is related to the transverse component of the relative momentum of the hadron pair via a new azimuthal angle. As a specific example, we spell out thoroughly the way to extract $h_1(x)$ from a measured single spin asymmetry in two-pion inclusive lepton-nucleon scattering. To estimate the sizes of observable effects we employ a spectator model for the fragmentation functions. The resulting asymmetry of our example is discussed as arising in different scenarios for the transversity.Comment: 17 pages, 15 figures in .eps format included, typesetted in RevTeX and epsfig.sty, submitted to Phys. Rev.

Lectures on conformal field theory and Kac-Moody algebras

This is an introduction to the basic ideas and to a few further selected topics in conformal quantum field theory and in the theory of Kac-Moody algebras.Comment: 59 pages, LaTeX2e, extended version of lectures given at the Graduate Course on Conformal Field Theory and Integrable Models (Budapest, August 1996), to appear in Springer Lecture Notes in Physic