Associated production of Higgs and single top at the LHC in presence of the SMEFT operators

We analyse the single top production in association with the Higgs at the Large Hadron Collider (LHC) using Standard Model (SM) effective operators upto dimension six. We show that the presence of effective operators can significantly alter the existing bound on the top-Higgs Yukawa coupling. We analyse events at the LHC with 35.9 and 137(140) fb$^{-1}$ integrated luminosities using both cut-based and machine learning techniques to probe new physics (NP) scale and operator coefficients addressing relevant SM background reduction. The four fermi effective operator(s) that contributes to the signal, turns out to be crucial and a bound on the operator coefficient is obtained from the present data and for future sensitivities.Comment: Version published in JHE

Endothelial PI3K-C2α, a class II PI3K, has an essential role in angiogenesis and vascular barrier function

The class II α-isoform of phosphatidylinositol 3-kinase (PI3K-C2α) is localized in endosomes, the trans-Golgi network and clathrin-coated vesicles; however, its functional role is not well understood. Global or endothelial-cell-specific deficiency of PI3K-C2α resulted in embryonic lethality caused by defects in sprouting angiogenesis and vascular maturation. PI3K-C2α knockdown in endothelial cells resulted in a decrease in the number of PI3-phosphate-enriched endosomes, impaired endosomal trafficking, defective delivery of VE-cadherin to endothelial cell junctions and defective junction assembly. PI3K-C2α knockdown also impaired endothelial cell signaling, including vascular endothelial growth factor receptor internalization and endosomal RhoA activation. Together, the effects of PI3K-C2α knockdown led to defective endothelial cell migration, proliferation, tube formation and barrier integrity. Endothelial PI3K-C2α deficiency in vivo suppressed postischemic and tumor angiogenesis and diminished vascular barrier function with a greatly augmented susceptibility to anaphylaxis and a higher incidence of dissecting aortic aneurysm formation in response to angiotensin II infusion. Thus, PI3K-C2α has a crucial role in vascular formation and barrier integrity and represents a new therapeutic target for vascular disease.In Press / 2013-03-18公開予定

Associated production of Higgs and single top at the LHC in presence of the SMEFT operators

Abstract We analyse the single top production in association with the Higgs at the Large Hadron Collider (LHC) using Standard Model (SM) effective operators upto dimension six. We show that the presence of effective operators can significantly alter the existing bound on the top-Higgs Yukawa coupling. We analyse events at the LHC with 35.9 and 137(140) fb −1 integrated luminosities using both cut-based and machine learning techniques to probe new physics (NP) scale and operator coefficients addressing relevant SM background reduction. The four fermi effective operator(s) that contribute to the signal, turn out to be crucial and a limit on the top-Higgs Yukawa interaction in presence of them is obtained from the present data and for future sensitivities

Red blood cell indices in different hemoglobinopathies: A cross-sectional study in Eastern India

Introduction: Beta thalassemia and hemoglobin (HbE)-related hemoglobinopathies are common public health problems in developing countries. High-performance liquid chromatography (HPLC) is currently the diagnostic test of choice for carrier detection, but it is costly. Hence, some initial screening and complementary tests are required, which can be affordable. Aims: To find out the distribution of different red blood cell (RBC) indices in beta thalassemia trait (BTT) and HbE-related hemoglobinopathies and to determine their significance as screening tests to distinguish between these hemoglobinopathies. Study Settings and Design: This observational cross-sectional study has been carried out at an NABL (National Accreditation Board for Testing and Calibration Laboratories)-accredited Laboratory of Eastern India with approval from the concerned Institutional Ethics Committee from January 2021 to March 2021. Methods and Material: HPLC tests and complete hemograms were performed on 2247 ethylenediaminetetraacetic acid anti-coagulated blood samples over 3 months. Patients 90% sensitivity and >80% specificity. Conclusion: RBC indices vary significantly between BTT and other HbE-related hemoglobinopathies. They can specially be utilized to differentiate BTT and E-trait as supportive tests in addition to the gold standard test of HPLC

Electrophoretic deposition of plasmonic nanocomposite for the fabrication of dye-sensitized solar cells

73-80TiO2-Ag nanocomposites have been prepared by hydrothermal process for the preparation of photoelectrode for dye-sensitized solar cells. The formation of TiO2-Ag nanocomposites have been confirmed by the transmission electron microscopy (TEM), UV-Vis spectroscopy, X-ray diffraction and energy dispersive X-ray analysis. The TEM images confirm that silver nanoparticles of average size 30 nm are dispersed inside the TiO2 matrix. Electrophoretic deposition technique (EPD) is successfully utilized to incorporate TiO2-Ag nanocomposites in commercially available TiO2 nanoparticle to prepare photoelectrode on transparent oxide substrate. Incorporation of TiO2-Ag nanocomposites by EPD technique has been done in different ways: in all the layers of TiO2 photoelectrode and in only the top layer of TiO2 photoelectrode. X-ray diffraction, field effect scanning electron microscopy in back scattered mode and photoluminescence (PL) spectroscopy study confirm the presence of TiO2-Ag nanostructure in prepared photoelectrode. The current-voltage characteristic shows 78% and 67% enhancement of photocurrent and power conversion efficiency (PCE) respectively in the DSSC with Ag incorporated photoelectrode compared to the cell without Ag nanoparticles and maximum PCE obtained in DSSC with TiO2-Ag is 7.5%

Genetic Characterization of Hepatitis B Virus in Peripheral Blood Leukocytes: Evidence for Selection and Compartmentalization of Viral Variants with the Immune Escape G145R Mutation▿ †

The compartmentalization of viral variants in distinct host tissues is a frequent event in many viral infections. Although hepatitis B virus (HBV) classically is considered hepatotropic, it has strong lymphotropic properties as well. However, unlike other viruses, molecular evolutionary studies to characterize HBV variants in compartments other than hepatocytes or sera have not been performed. The present work attempted to characterize HBV sequences from the peripheral blood leukocytes (PBL) of a large set of subjects, using advanced molecular biology and computational methods. The results of this study revealed the exclusive compartmentalization of HBV subgenotype Ae/A2-specific sequences with a potent immune escape G145R mutation in the PBL of the majority of the subjects. Interestingly, entirely different HBV genotypes/subgenotypes (C, D, or Aa/A1) were found to predominate in the sera of the same study populations. These results suggest that subgenotype Ae/A2 is selectively archived in the PBL, and the high prevalence of G145R indicates high immune pressure and high evolutionary rates of HBV DNA in the PBL. The results are analogous to available literature on the compartmentalization of other viruses. The present work thus provides evidence in favor of the compartment-specific abundance, evolution, and emergence of the potent immune escape mutant. These findings have important implications in the field of HBV molecular epidemiology, transmission, transfusion medicine, organ transplantation, and vaccination strategies

Acquisition of classical CTX prophage from Vibrio cholerae O141 by El Tor strains aided by lytic phages and chitin-induced competence

The El Tor biotype of Vibrio cholerae O1, causing the current seventh pandemic of cholera, has replaced the classical biotype, which caused the sixth pandemic. The CTX prophages encoding cholera toxin in the two biotypes have distinct repressor (rstR) genes. Recently, new variants of El Tor strains that carry the classical type (CTXclass) prophage have emerged. These "hybrid" strains apparently originate through lateral gene transfer and recombination events. To explore possible donors of the CTXclass prophage and its mode of transfer, we tested environmental V. cholerae isolates for the presence of CTXclass prophage and mobility of the phage genome. Of the 272 environmental V. cholerae isolates tested, 6 were found to carry the CTXclass prophage; all of these belonged to the O141 serogroup. These O141 strains were unable to produce infectious CTXclass phage or to transmit the prophage to recipient strains in the mouse model of infection; however, the CTXclass prophage was acquired by El Tor strains when cultured with the O141 strains in microcosms composed of filtered environmental water, a chitin substrate, and a V. cholerae O141-specific bacteriophage. The CTXclass prophage either coexisted with or replaced the resident CTXET prophage, resulting in El Tor strains with CTX genotypes similar to those of the naturally occurring hybrid strains. Our results support a model involving phages and natural chitin substrate in the emergence of new variants of pathogenic V. cholerae. Furthermore, the O141 strains apparently represent an alternative reservoir of the CTXclass phage genome, because the classical V. cholerae O1 strains are possibly extinct

An improved technique for isolation of environmental vibrio cholerae with epidemic potential: monitoring the emergence of a multiple-antibiotic-resistant epidemic strain in Bangladesh

Predicting cholera epidemics through monitoring the environment for the presence of pathogenic Vibrio cholerae is complicated by the presence in water of a large number of mostly nonpathogenic V. cholerae strains. V. cholerae strains causing recent cholera epidemics in Bangladesh carry the sulfamethoxazole-trimethoprim (SXT) element, which encodes resistance to several antibiotics. Here, we show that the use of a culture medium containing streptomycin, sulfamethoxazole, and trimethoprim (the antibiotic selection technique [AST]) can significantly enhance the isolation of environmental V. cholerae O1 with epidemic potential (P<.001). The AST was also used to monitor the recent emergence and spread of a new multiple-antibiotic-resistant strain of V. cholerae in Bangladesh. The results of this study support the hypothesis that pre-epidemic amplification of pathogenic V. cholerae occurs in the human host and leads to the start of an epidemic cycle dominated by a single clone of V. cholerae that spreads rapidly through environmental waters