Development Of A Portable and Rapid Decalcification Device For Oral Hard Tissue Specimens

Decalcification is a routinely carried out procedure by oral pathologists, as the lesions involving the pulp can only be seen and identified through decalcified sections. The present methods involve usage of chemical agents, either with inorganic acids which dissolves the calcium to form soluble calcium salts or with chelating agents. Even though the chelating agents preserve the tissue morphology and show excellent microscopic picture, it has less diagnostic efficacy, as the time taken for it to decalcify the specimens is considerably high. It becomes important for us to develop a decalcification technique which will be faster and will maintain the balance between decalcification speed and tissue morphology. Hence, the aim of this study was to develop a portable rapid decalcification unit and compare the efficacy among all the three-decalcification procedure using teeth and bone specimens, namely the conventional method, microwave method and electrolytic method

Anticancer and antioxidant activity of ethanolic extract of Clove (Syzygium aromaticum) on oral squamous cell carcinoma cell lines (KB cell lines)- An In-vitro study

Cloves (Syzygium aromaticum) as been used as traditional medicine for many years and they possess antibacterial, antifungal and antiviral properties. Clove is known for its anticancer property on various cancer cell lines and is well established, but its anticancer effect on OSCC cell lines is less known.  Aim of the study was to determine the anticancer and antioxidant effect of Syzygium aromaticum extract on OSCC cell lines (KB cell lines) and compare the same with normal mouse fibroblasts cell lines (L292 cell lines). KB cell lines and L292 cell lines were commercially obtained.  Clove was obtained from local market and ethanolic extract (EC) of clove was prepared. Anticancer activity was assessed by MTT, neutral red, DAPI and Double staining assay and antioxidant assay was carried out by FRAP, PM and DPPH assay. The antioxidant property of EC of clove increased with increase in the concentration in a dose dependent manner. Both MTT and Neutral Red assay showed increase in cell death with increase in concentration of EC of clove. Double staining and DAPI showed increase in cell death when treated with EC of clove. The anticancer and antioxidant activity of EC of clove was comparable with standard drug used in the assay. This in vitro study demonstrates effective anticancer and antioxidant activity on KB cell lines when compared to standard control. However, further studies are to be conducted in order to characterize other potential antitumor components of the clove, so that it can be used as therapeutic agent in treating oral carcinoma

Culture-based identification of pigmented Porphyromonas and Prevotella species in primary endodontic infections

Background. The most common species isolated from primary endodontic infections are black-pigmented bacteria. These species are implicated in apical abscess formation due to their proteolytic activity and are fastidious in nature. Therefore, the present study was carried out to evaluate the presence and identification of various pigmented Porphyromonas and Prevotella species in the infected root canal through culture-based techniques. Methods. Thirty-one patients with primary endodontic infections were selected. Using sterile paper points, samples were collected from the root canals after access opening and prior to obturation, which were cultured using blood and kanamycin blood agar. Subsequently, biochemical test was used to identify the species and the results were analyzed using percentage comparison analysis, McNemar and chi-squared tests, Wilcoxon match pair test and paired t-test. Results. Out of 31 samples 26 were positive for black-pigmented organisms; the predominantly isolated species were Prevotella followed by Porphyromonas. In Porphyromonas only P. gingivalis was isolated. One of the interesting features was isolation of P. gingivalis through culture, which is otherwise very difficult to isolate through culture. Conclusion. The presence of Prevotella and Porphyromonas species suggests that a significant role is played by these organisms in the pathogenesis of endodontic infections

Model to estimate the salt and pepper noise density level on gray-scale digital image

In this research paper, we proposed a probabilistic analysis to find the relationship between entropy of image and salt & pepper noise density. For this estimation, we have employed entropy inspection of spatial domain technique. Based on the fact that entropy of image signal decreases with increase in noise density and this decreasing relationship between noise and entropy is robust to individual images traits. In this work, we exploited the entropy values of noisy image with respect to its noise density, and analyzed that such relation is robust to individual images. Further, we considered such relationships for estimation of noise level. Based on the numerical calculations and graphical representations it reveals to the fact that the error is reduced to 8.9% which can be considered as an appropriate model to estimate the salt and pepper noise density

Assessment of the effect of frequency of low-level laser therapy exposure at different intervals on orthodontic tooth movement – A systematic review and meta-analysis

OBJECTIVE: To assess the optimal intervals of exposure of low-level LASER therapy (LLLT) that would optimally accelerate orthodontic tooth movement. Second objective was to quantitatively analyze the difference in the time taken for alignment of anterior teeth with and without the application of LASER. MATERIALS AND METHODS: PROSPERO database registry was done (CRD42020196472) and review was conducted based on PRISMA guidelines. A search was systematically conducted in five major electronic databases without restrictions up to June 2020 along with a hand search of selected journals. The quality of evidence was assessed using the Grading of Recommended Assessment, Development, and Evaluation tool, risk of bias using Cochrane risk of bias tool, and meta-analysis was carried out using RevMan 5.4 software. RESULTS: Ten randomized controlled trials which met the inclusion criteria were evaluated and tabulated. A random-effects meta-analysis demonstrated that there is a statistically significant increase in the orthodontic tooth movement when patients were exposed to minimum of four intervals of LLLT in the first month, at P =0.03 by a standard mean difference of 0.46 mm with an overall heterogeneity of I2 = 0% at 95% confidence interval. There was a statistically highly significant reduction in the number of days taken for alignment of anterior teeth with the application of LASER (P < .00001). CONCLUSION: Application of LLLT for minimum of four irradiations in the first month has yielded better results in accelerating orthodontic tooth movement than application of LLLT once a month

A Perspective of the Pore Architecture of Nanoporous Materials by Vapour Adsorption of Aromatics and Olefins

Three nanoporous adsorbent materials, viz. beta zeolite, porous clay hetero-structure (PCH) and hexadecyltrimethylammonium cation-intercalated montmorillonite (Mont-2), having different pore architectures were used in the investigation. The adsorption of benzene, C 1 –C 4 alkyl-substituted benzenes, C 6 –C 9 straight-chain alkenes and isomeric hexenes was studied using a McBain–Bakr gravimetric balance. Interesting observations were made with respect to the adsorption uptake of the different probe molecules for exploring the pore architecture. Beta zeolite is a large pore zeolite (7.5 Å) which allows three-dimensional access to molecules, whereas PCH is known to have large pores allowing two-dimensional access for molecular entry. Irrespective of the number of attached –CH 2 side-chain groups attached, the rate of diffusion of the molecules inside the beta zeolite pore channels was less constrained. However, in the case of Mont-2, where the pores were blocked by the surfactant molecules, adsorption was apparently due to the diffusion of molecules through the interlayer packed with a bilayer of surfactant molecules. The packing density of the adsorbate molecules in the channels of the adsorbent was determined by the space-filling characteristics of the molecules which, in turn, depended on various factors such as the effective molecular volume, steric factors, the positioning of the double bonds, and the attractive and repulsive forces