Perfil epidemiológico de óbitos por HIV/AIDS na região nordeste do Brasil utilizando dados do sistema de informação de saúde do DATASUS / Epidemiological profile of HIV/AIDS deaths in northeastern Brazil using data from the DATASUS health information system

Introdução: O HIV/aids é um problema de saúde pública por suas complicações e altos custos gerados pela doença. A mortalidade dela no Brasil (BR) tem diminuído devido a assistência de saúde, mas isso difere entre as regiões, tendo o Nordeste (NE) com aumento de óbitos. Objetivo: Identificar a epidemiologia dos óbitos por HIV/aids no NE. Métodos: É um estudo epidemiológico descritivo com dados de óbitos por HIV/aids no NE obtidos através do Departamento de Informática do Sistema Único de Saúde (DATASUS) no período de 2006 – 2016. Resultados: A quantidade de óbitos por HIV/aids nos estados do NE foram: Paraíba-PB (1.295); Rio Grande do Norte-RN (1.019); Pernambuco-PE (7.305); Alagoas-AL (807); Maranhão-MA (4.916); Sergipe-SE (674) e Piauí-PI (1.012). Neles, cerca de 90% estavam entre 20-60 anos e o sexo masculino com aproximadamente 70%. Na escolaridade, 50-60% com 1-7 anos de estudos e cerca de 20% tanto para sem informação como para 8-11 anos de estudos. Nessas regiões, 60-75% eram pardos, com exceção de PE, com 64,4% amarelos. Em todos os locais, cerca de 70% eram solteiros e em torno de 20% casados. Conclusão:Diante deste estudo, notou-se que a assistência da saúde aos pacientes HIV/aids não é universal e equitativa no BR, dificultando o acesso ao diagnóstico e, consequentemente, um início tardio do tratamento. Portanto, é essencial ações de políticas públicas e dos profissionais para a melhoria do suporte à saúde.

“SUPORTE NA ATENÇÃO BÁSICA E OS DESAFIOS NA SUA INTEGRALIDADE COM ÊNFASE NA MATRICIAL

to investigate the development of matrix support activities between the Expanded Family Health Center, Psychosocial Care Center and Family Health Strategy Teams. Method: This is a qualitative research developed from the perspective of institutional analysis in the municipality of Currais Novos, in the interior of Rio Grande do Norte, between June and September 2020. Semi-structured interviews were carried out with 22 professionals from the aforementioned health services. Results: Three categories were identified as a result of content analysis from the stories obtained in the interviews: weaknesses and challenges in mental health intake practices. Matrix support and paths forward. Fragmented attention and lack of responsibility for care. Conclusion: It was possible to verify that matrix support still has a long way to go so that the way in which requirements and equipment are guided and articulated is truly recommended. investigar o desenvolvimento das atividades do matriciamento entre Núcleo Ampliado de Saúde da Família, Centro de Atenção Psicossocial e Equipes da Estratégia de Saúde da Família. Método: Trata-se de uma pesquisa qualitativa desenvolvida na perspectiva da análise institucional no município de Currais Novos, interior do Rio Grande do Norte, entre junho e setembro de 2020. Foram realizadas entrevistas semiestruturadas com 22 profissionais dos referidos serviços de saúde. Resultados: Três categorias foram identificadas como resultado da análise de conteúdo a partir das estórias obtidas nas entrevistas: fraquezas e desafios nas práticas de ingestão de saúde mental. Apoio matricial e caminhos a seguir. Atenção fragmentada e falta de responsabilidade pelo cuidado. Conclusão: Foi possível constatar que o apoio matricial ainda tem um longo caminho a percorrer para que a forma como são guiados e articulados requisitos e equipamentos seja realmente o recomendado

A cirurgia ortognática no tratamento da Apneia Obstrutiva do Sono: Uma revisão integrativa da literatura

The aim of this review is to survey the most recent scientific evidence on the effectiveness oforthognathic surgery in the treatment of OSA. The guiding question was: "What are the mostrecent scientific documents on the effectiveness of orthognathic surgery in the treatment ofOSA in adult patients?". Thus, the eligibility criteria consisted of only original articles relatedto the guiding question, available in full for free in any language in the last five years. Theselection of articles was carried out from searches in the PubMed, Scopus, Embase and Webof Science databases. A combination of four descriptors was used: (Orthognathic Surgery) AND(Obstructive Sleep Apnea) AND (Maxillomandibular Advancement) AND (Treatment). TheMMA is a safe effective treatment option for cases of AOS. Being the main alternative to longterm treatments for severe cases of OSA.O objetivo desta revisão é fazer um levantamento das evidências científicas mais recentes sobrea eficácia da cirurgia ortognática no tratamento da AOS. A questão norteadora foi: "Quais sãoevidências científicas mais recentes sobre a eficácia da cirurgia ortognática no tratamento da AOSem pacientes adultos?”. Dessa maneira, os critérios de elegibilidade consistiram em apenasartigos originais relacionados com a questão norteadora, disponíveis na íntegra de forma gratuitaem qualquer idioma nos últimos cinco anos. A seleção dos artigos foi realizada a partir de buscasnas bases de dados PubMed, Scopus, Embase e Web of Science. Utilizou-se a combinação dequatro descritores: (Orthognathic Surgery) AND (Obstructive Sleep Apnea) AND(Maxillomandibular Advancement) AND (Treatment). O AMM apresenta-se como uma opçãosegura e eficaz de tratamento para os casos de AOS. Sendo a principal alternativa aostratamentos a longo prazo para casos graves de AOS

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5,6,7 vast areas of the tropics remain understudied.8,9,10,11 In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepresented in biodiversity databases.13,14,15 To worsen this situation, human-induced modifications16,17 may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%–18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5,6,7 vast areas of the tropics remain understudied.8,9,10,11 In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepresented in biodiversity databases.13,14,15 To worsen this situation, human-induced modifications16,17 may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%–18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost

Seminal plasma proteins and metabolites: Effects on sperm function and potential as fertility markers

Molecular components of sperm and in the media surrounding them influence male fertility. In this regard, seminal plasma proteins and metabolites modulate various reproductive events, including sperm motility and capacitation, cell protection, acrosome reaction, fertilization and embryonic development. Empirical associations between seminal proteins and metabolites and fertility indicate that these molecules are potential molecular markers of male reproductive status in cattle and other species

Methods for obtaining the enriched fraction of ram seminal vesicle proteins (RSVP14)

The objective of the present study was to develop a methodology to obtain the enriched fraction of ram seminal vesicle protein 14 (RSVP14). The study was developed using Morada Nova rams, from which semen samples were collected weekly. Seminal plasma proteins were precipitated with cold ethanol, and then 6.15 mg/mL of total proteins were subjected to liquid gelatin affinity chromatography using a GelatinSepharose matrix coupled to an automated chromatographic system. Proteins were eluted into four fractions (A, B, C, and D), in which A and B contained non-gelatin-binding proteins, and C and D fractions contained gelatin-binding proteins. Gels were analyzed by Quantity One software, in which five protein bands were detected in fraction D, with molecular weights between 12 and 30 kDa. The gelatin-binding proteins (fraction D) were loaded into a HiTrap™ Heparin HP affinity column. Two chromatographic fractions were separated (D1 and D2), in which D1 contained non-heparin-binding proteins, and D2 contained heparin-binding proteins. Proteins from the last two peaks were subjected to 12.5% SDS-PAGE and Western Blot. Two bands with molecular weight of 14 and 24 kDa, contained in fraction D1, were excised from gel and subjected to tandem mass spectrometry, identifying the proteins RSVP14 and RSVP24. Thus, the chromatographic methods of the present study are efficient to capture the enriched fraction of RSVP1

Crystallization and preliminary X-ray diffraction analysis of the lectin from Dioclea rostrata Benth seeds

D. rostrata lectin was crystallized by hanging-drop vapor diffusion. The crystal belongs to the orthorhombic space group I222 and diffracted to 1.87 Å resolution