3-D polymeric microfluidic devices for BioMOEMS applications

11 páginas, 12 figuras.-- Trabajo presentado en la conferencia "Bioengineered and Bioinspired Systems II"; Sevilla (España); 09-Mayo-2005; Editores: Ricardo A. Carmona, Gustavo Linan-Cembrano.This paper describes the fabrication, packaging and characterization of novel multilayer polymer microfluidic systems fabricated by a CMOS compatible process. These microfluidic devices were specially designed for BioMOEMS applications. Embedded multilayer rectangular smooth and uniform microchannels, 50 to 150 mm wide and 18mm deep were studied. Steady-state flow rates and pressure driven flow control were measured in the laminar flow regime. Flow rates ranging from 1 to 100 µl/min, at pressure drop ranging from 10 to 600 kPa, were obtained. These flow rates yield Reynolds numbers (Re) up to 20. Results indicate that the experimental Re and the flow friction coefficient (f) are in good agreement with the laminar flow theory. These experimental results facilitate the future designs of different microfluidic devices designed by using classical fluidic theory. We also present two different methods developed for macro/microfluidic packaging in order to connect these microfluidic devices to the macroscopic world. The microsystem packaging can withstand pressure drops up from 500 to 2000 kPa with any liquid leakage.This research is sponsored by the Basque and Spanish Governments, under the Torres Quevedo Spanish Fellowship for industrial research and the strategic research program on micro and nanotechnologies (MICROGUNE).Peer reviewe

Optimizing polymer lab-on-chip platforms for ultrasonic manipulation: Influence of the substrate

The choice of substrate material in a chip that combines ultrasound with microfluidics for handling biological and synthetic microparticles can have a profound effect on the performance of the device. This is due to the high surface-to-volume ratio that exists within such small structures and acquires particular relevance in polymer-based resonators with 3D standing waves. This paper presents three chips developed to perform particle flow-through separation by ultrasound based on a polymeric SU-8 layer containing channelization over three different substrates: Polymethyl methacrylate (PMMA); Pyrex; and a cracked PMMA composite-like structure. Through direct observations of polystyrene microbeads inside the channel, the three checked chips exhibit their potential as disposable continuous concentration devices with different spatial pressure patterns at frequencies of resonance close to 1 Mhz. Chips with Pyrex and cracked PMMA substrates show restrictions on the number of pressure nodes established in the channel associated with the inhibition of 3D modes in the solid structure. The glass-substrate chip presents some advantages associated with lower energy requirements to collect particles. According to the results, the use of polymer-based chips with rigid substrates can be advantageous for applications that require short treatment times (clinical tests handling human samples) and low-cost fabrication. © 2015 by the authors; licensee MDPI, Basel, Switzerland.The study has been performed in the framework of two Spanish National Research Project BIO2011-30535-C04-01,02,03, “Development of a high throughput for isolation of tumor cells circulating in peripheral blood”.We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI).Peer Reviewe

Microcámara y dispositivo de cultivo celular monitorizables por resonancia magnética nuclear

La presente invención hace referencia a una microcámara ya un dispositivo de cultivo celular, monitorizables por resonancia magnética nuclear y otras técnicas de imagen, donde dicha microcámara de cultivo se encuentra encapsulada y alojada en el interior de un chip. Dicha microcámara y dicho dispositivo resultan de fácil manejo para el usuario, permitiendo su manipulación o su reemplazo sin la necesidad de un montaje laborioso, beneficiando además notablemente el estudio de cultivos durante periodos largos de tiempo, superiores a 24 horas.Peer reviewedCentro de Investigación Biomecánica en Red en Bioingeniería Biomateriales y Nanomedicina, Universitat de Valencia, Consejo Superior de Investigaciones Científicas (España) , IKERLAN S. COOP.A1 Solicitud de patente con informe sobre el estado de la técnic

3-D polymeric microfluidic devices for BioMOEMS applications

11 páginas, 12 figuras.-- Trabajo presentado en la conferencia "Bioengineered and Bioinspired Systems II"; Sevilla (España); 09-Mayo-2005; Editores: Ricardo A. Carmona, Gustavo Linan-Cembrano.This paper describes the fabrication, packaging and characterization of novel multilayer polymer microfluidic systems fabricated by a CMOS compatible process. These microfluidic devices were specially designed for BioMOEMS applications. Embedded multilayer rectangular smooth and uniform microchannels, 50 to 150 mm wide and 18mm deep were studied. Steady-state flow rates and pressure driven flow control were measured in the laminar flow regime. Flow rates ranging from 1 to 100 µl/min, at pressure drop ranging from 10 to 600 kPa, were obtained. These flow rates yield Reynolds numbers (Re) up to 20. Results indicate that the experimental Re and the flow friction coefficient (f) are in good agreement with the laminar flow theory. These experimental results facilitate the future designs of different microfluidic devices designed by using classical fluidic theory. We also present two different methods developed for macro/microfluidic packaging in order to connect these microfluidic devices to the macroscopic world. The microsystem packaging can withstand pressure drops up from 500 to 2000 kPa with any liquid leakage.This research is sponsored by the Basque and Spanish Governments, under the Torres Quevedo Spanish Fellowship for industrial research and the strategic research program on micro and nanotechnologies (MICROGUNE).Peer reviewe

Microdispositivo de separación y extracción selectiva y no invasiva de partículas en suspensiones polidispersas, procedimiento de fabricación y sus aplicaciones

Microdispositivo de separación y extracción selectiva y no invasiva de par tículas en suspensiones polidispersas, procedimiento de fabricación y sus aplicaciones. La presente invención describe un microdispositivo de se- paración y extracción no invasiva y selectiva de par tículas en suspensiones polidispersas mediante el uso estraté- gico de ultrasonidos, flujo laminar y efectos de onda es- tacionaria establecidos en un canal realizado en micro- tecnología en un chip. Este dispositivo tipo lamda cuar tos modificado permite canalizar y separar las par tículas en un flujo interior del canal del sustrato sin tocar las pare- des del dispositivo, por lo que son menos dañadas. Este microdispositivo puede utilizarse en el campo de la bio- medicina y/o biotecnología para la separación y concen- tración de células, preferentemente humanas, útiles para procedimientos de investigación y médicos de diagnóstico y tratamiento.Consejo Superior de Investigaciones Científicas (España), Ikerlan, Universidad Miguel Hernández, Hospital General Universitario de ElcheB1 Patente sin examen previ

Microagujas de SU-8 para la monitorización y la estimulación neuronal

Microaguja de SU-8 para la monitorización y estimulación neuronal de espesor inferior a 100 micrómetros y largura desde 50 micras hasta los 10 centímetros, cuyo procedimiento de fabricación permite extraer la microaguja del sustrato sin utilizar medios mecánicos gracias al recubrimiento inicial de un sustrato rígido con una capa de aluminio, como capa sacrificial, y el ataque químico final de la capa de Aluminio para la extracción química de la microaguja obtenida en etapas intermedias conocidas de fabricación por fotolitografía.Peer reviewedConsejo Superior de Investigaciones Científicas (España), KERLAN S COOPA1 Solicitud de patentes con informe sobre el estado de la técnic

Microcámara y dispositivo de cultivo celular monitorizables por resonancia magnética nuclear

[EN] The invention relates to a cell culture device and microchamber which can be monitored using nuclear magnetic resonance and other imaging techniques, in which the culture microchamber is encapsulated and housed inside a chip. The microchamber and the device are easy for the user to handle, allowing same to be handled or repositioned without requiring complex mounting operations. In addition, the invention allows cultures to be studied for long periods, greater than 24 hours.[ES] La presente invención hace referencia a una micro cámara y a un dispositivo de cultivo celular, monitorizables por resonancia magnética nuclear y otras técnicas de imagen, donde dicha micro cámara de cultivo se encuentra encapsulada y alojada en el interior de un chip. Dicha micro cámara y dicho dispositivo resultan de fácil manejo para el usuario, pennitiendo su manipulación o su reemplazo sin la necesidad de un montaje laborioso, beneficiando además notablemente el estudio de cultivos durante periodos largos de tiempo, superiores a 24 horas.Peer reviewedCentro de Investigación Biomédica en Red en Bioingeniería Biomateriales y Nanomedicina, Universitat de Valencia, Consejo Superior de Investigaciones Científicas (España), Ikerlan S CCOPA1 Solicitud de patente con informe sobre el estado de la técnic

A polymeric chip for micromanipulation and particle sorting by ultrasounds based on a multilayer configuration

A new polymer microchip is presented in this paper for separation and particle sorting in flowing suspensions. It includes a microchannel where a fluid-sample containing particles flows in parallel with another liquid. The working principle is based on an ultrasonic actuation on the cross-section of the chip, which behaves as a multilayer system. That part of the wave established within the channel includes a node of pressure strategically located within the collector fluid path, where the target particles collected to be extracted from their host sample. The use of a polymer as the constitutive material of the chip, new in this type of devices, allows a channel width somewhat larger than a quarter of a wavelength, which does not fit any of the conventional models but it represents an intermediate situation. The device has been fabricated by standard SU-8 photolithography using PMMA as substrate. In the experiments, polystyrene particles of 20 μm in diameter have been extracted from aqueous suspensions containing 6-μm sized particles by a strategic application of ultrasonic waves. A high efficiency of particle separation, over 95%, at different concentrations and flow rates, prove the feasibility of the device to carry out sorting processes on flowing suspensions.This trial was performed partly within the Transversal Cancer Action approved by the Council of Ministers on October 11, 2007, in accordance with an agreement between the Carlos III Health Institute and the Biomedical Research Foundation of Hospital of Elche. Part of this research was performed within a National Research Project BIO2008-06843-C04 funded by the Science and Innovation Ministry of Spain.Peer reviewe

Microfluidic-optical integrated CMOS compatible devices for label-free biochemical sensing

11 páginas, 17 figuras, 1 tabla.The fabrication, characterization and packaging of novel microfluidic-optical integrated biosensors for label-free biochemical detection is presented in this paper. The integrated device consists of a three-dimensional embedded microchannel network fabricated using enhanced CMOS compatible SU-8 multilevel polymer technology on top of a wafer containing Mach-Zehnder Interferometer (MZI) nanophotonic biosensor devices. PMMA housing provides connection to the macro-world and ensures robust leakage-free flow operation of the devices. This macro-microfluidic module can operate at pressure drops up to 1000 kPa. Fluid flow experiments have been performed in order to demonstrate the robustness of our microfluidic devices. The devices have been designed to operate under continuous flow. Steady-state flow rates ranging from 1 to 100 µl min−1 at pressure drops ranging from 10 to 500 kPa were measured in the laminar flow regime. Experimental results are in good agreement with laminar flow theory. The first interferometric sensing measurements are presented in order to demonstrate the functionality of these novel integrated devices for lab-on-a-chip and label-free biosensing applications. A bulk refractive index detection limit of 3.8 × 10−6 was obtained, close to the minimum detected up to now by label-free biosensor devices without microfluidic integration. As far as we know, this is the first time that a label-free biosensor device is integrated within a microfluidic network using a wafer-level CMOS compatible process technology.This research is sponsored by the Basque and Spanish Governments, under a Torres Quevedo Spanish Fellowship for industrial research and the programs of research and development ofmicro and biotechnologies MICROGUNE and BIOGUNE.Peer reviewe

Microchip multisensor de medida de flujo, temperatura y concentración de gases para el control de la combustión, procedimiento de fabricación y sus aplicaciones

Fecha de presentación internacional: 06.10.2004. - Titulares: Consejo Superior de Investigaciones Científicas (CSIC). - Copreci S. COOP. - Gas Natural SDA, S.A. - Universidad de Barcelona. - Fagor electrodomésticos S. COOP.[EN] The invention relates to the design and production of a microelectronic chip comprising an integrated assembly of gas flow and temperature sensors. The inventive microchip can be used to determine the concentrations of different gases in an environment, as well as the flow rates and temperatures thereof. The aforementioned measurements can be used to monitor combustion in a household furnace and to adjust the operation thereof in order to obtain safe, low-emission combustion.[ES] En la presente invención se reivindica el diseño y fabricación de un chip microelectrónico que integra un conjunto de sensores de gases, flujo y temperatura. Con este nuevo microchip es posible determinar las concentraciones de diversos gases en un ambiente, su caudal y temperatura. Dichas medidas permiten monitorizar la combustión en una caldera doméstica y actuar sobre su funcionamiento para conseguir una combustión baja en emisiones y segura.Peer reviewe