Biological heterogeneity of putative bladder cancer stem-like cell populations from human bladder transitional cell carcinoma samples.

Transitional cell carcinoma (TCC) is the most common type of bladder cancer. Emerging evidence has suggested that the capability of a tumor to grow and propagate is dependent on a small subset of cells, the cancer stem-like cells (CSCs) or tumor initiating cells. We report on the isolation and biological characterization of putative bladder CSC populations from primary TCCs. Isolated cells were induced to proliferate in stem cell culture conditions (serum-free medium containing mitogenic growth factors). The proliferating cells formed spheroids (urospheres) and their abilities for extensive proliferation and self-renewal were assayed. Their positivity for several stem cell markers (CD133, Oct-3/4, nestin, and cytokeratins) was also assessed by immunofluorescence tests and they could have the potential to differentiate in the presence of serum. In stem cell culture conditions they gradually showed loss of proliferation, adherence to the substrate, and morphological changes, which might reflect their progressive acquisition of differentiative capacity and loss of self-renewal ability. To evaluate if effective cell selection occurred after isolation, conventional cytogenetic studies on fresh chromosome spreads immediately after isolation and after culture were carried out. In addition, a molecular cytogenetic study by UroVysion assay was carried out on paraffin-embedded tissue sections and on fresh and after culture nuclei preparations. The data collected indicated important karyotype changes and a positive selection for hypo- or near-diploid cells, losing the complexity present in fresh tumors

3D Microfluidic model for evaluating immunotherapy efficacy by tracking dendritic cell behaviour toward tumor cells

Immunotherapy efficacy relies on the crosstalk within the tumor microenvironment between cancer and dendritic cells (DCs) resulting in the induction of a potent and effective antitumor response. DCs have the specific role of recognizing cancer cells, taking up tumor antigens (Ags) and then migrating to lymph nodes for Ag (cross)-presentation to naïve T cells. Interferon-α-conditioned DCs (IFN-DCs) exhibit marked phagocytic activity and the special ability of inducing Ag-specific T-cell response. Here, we have developed a novel microfluidic platform recreating tightly interconnected cancer and immune systems with specific 3D environmental properties, for tracking human DC behaviour toward tumor cells. By combining our microfluidic platform with advanced microscopy and a revised cell tracking analysis algorithm, it was possible to evaluate the guided efficient motion of IFN-DCs toward drug-treated cancer cells and the succeeding phagocytosis events. Overall, this platform allowed the dissection of IFN-DC-cancer cell interactions within 3D tumor spaces, with the discovery of major underlying factors such as CXCR4 involvement and underscored its potential as an innovative tool to assess the efficacy of immunotherapeutic approaches

Estomatotrivias: aplicación de un juego digital en alumnos de educación superior

Introducción. La tecnología, ha cambiado la forma en que concebimos la vida y el mundo. Según Bruner (1997), si bien el aprendizaje está en la mente, tiene su origen en la cultura en la que se crea. De ésta manera “las construcciones impuestas por los sistemas simbólicos accesibles a la mente humana, en general impuestos por la cultura” originan y limitan la construcción del aprendizaje. Por lo tanto, el alumnado no queda exento de éste cambio y por éste motivo debemos comenzar a replantearnos el uso de la tecnología y el juego dentro del proceso de enseñanza-aprendizaje, adquiriendo técnicas y métodos educativos relacionados al uso de las nuevas herramientas que vienen surgiendo. Objetivo. Presentar una experiencia educativa tecnológica que fortalezca el desarrollo del aprendizaje significativo en los alumnos.Facultad de Odontologí

Effectiveness and safety of filgotinib in rheumatoid arthritis: a real-life multicentre experience

Objectives: We investigated the effectiveness and safety of filgotinib in a real-life multicentre cohort of rheumatoid arthritis (RA) patients. Methods: RA patients were evaluated at baseline and after 12 and 24 weeks and were stratified based on previous treatments as biologic disease-modifying anti-rheumatic drug (bDMARD)-naive and bDMARD-insufficient responders (IR). Concomitant usage of methotrexate (MTX) and oral glucocorticoids (GC) was recorded. At each timepoint we recorded disease activity, laboratory parameters and adverse events. Results: 126 patients were enrolled. 15.8% were bDMARD-naive (G0), while 84% were bDMARD-IR (G1). In G0, 45% of patients were in monotherapy (G2) and 55% were taken MTX (G3). In G1, 50% of patients were in monotherapy (G4) and 50% used MTX (G5).A significant reduction in all parameters at 12 weeks was observed; in the extension to 24 weeks the significant reduction was maintained for patient global assessment (PGA), examiner global assessment (EGA), visual analogue scale (VAS) pain, VAS fatigue, disease activity score (DAS)28- C-reactive protein (CRP) and CRP values. Filgotinib in monotherapy showed better outcomes in bDMARD-naive patients, with significant differences for patient reported outcomes (PROs) and DAS28-CRP. At 12 weeks, low disease activity (LDA) and remission were achieved in a percentage of 37.2 % and 10.7 % by simplified disease activity index (SDAI), 42.6 % and 5.7 % by clinical disease activity index (CDAI), 26.8 % and 25.2 % by DAS28-CRP, respectively. A significant decrease in steroid dose was evidenced in all patients. We observed a major adverse cardiovascular event in one patient and an increase in transaminase in another. No infections from Herpes Zoster were reported. Conclusions: Our real-world data confirm the effectiveness and safety of filgotinib in the management of RA, especially in bDMARD-naive patients

Saltando hacia la salud : Salta sonríe con Boquitas Saludables

Objetivo general del proyecto: Crear un vinculo de interacción reciproca entre la comunidad y los integrantes del proyecto con la finalidad de promover, elevar y sostener los niveles de salud en la comunidad de manera que se mantengan en el tiempo, utilizando técnicas preventivas y curativas que garanticen la permanencia y replicabilidad del mensaje de salud en la comunidad.Facultad de Odontologí

Incluir para sonreír

Objetivo general del proyecto: Prevenir enfermedades gingivodentales relacionadas con el uso de piercing, con participación comunitaria que garantice un impacto en la salud de la población.Facultad de Odontologí

Incluir para sonreír

Objetivo general del proyecto: Prevenir enfermedades gingivodentales relacionadas con el uso de piercing, con participación comunitaria que garantice un impacto en la salud de la población.Facultad de Odontologí

Odontoprotesistas al rescate : Devolviendo sonrisas II

Introducción. Devolviendo Sonrisas es una iniciativa que comenzó hace varios años desde la Asignatura Prótesis B, y por una demanda Social de personas de la 3ra edad con necesidad de restituir sus piezas dentarias perdidas a través de elementos protésicos. Con la intención de poder brindar ayuda a más individuos, generamos una nueva propuesta donde primase la resolución de una problemática creciente, que es la falta de inserción en el mercado laboral de adultos jóvenes que, por falta de Piezas dentarias son excluidos. Consideramos que esta ausencia conlleva un sinnúmero de problemáticas tanto a nivel físico como psicosocial. De ahí la importancia de fortalecer las políticas públicas de atención de la Salud resolviendo estas necesidades. Objetivos. Elevar los niveles de salud de la población realizando tratamientos que contemplen la eliminación de patologías orales y la posterior confección, prueba e instalación de prótesis completas y parciales de acrílico.Facultad de Odontologí

Devolviendo Sonrisas. Parte III : Odontoprotesistas al rescate

Este proyecto se presentó por demanda de un grupo de personas nucleadas en el Barrio San Carlos de la Plata (Las Quintas y El Triunfo) que a través del Programa “Barrio Adentro” dependiente del Organismo Provincial de Niñez y Adolescencia, perteneciente al ministerio de Desarrollo de la Comunidad de la Provincia de Buenos Aires, quienes detectaron la necesidad de implementar acciones sanitarias en niñas y niños jóvenes socialmente vulnerables y sus familias. Las acciones se abocaron en restituir piezas dentarias perdidas a través de elementos protésicos. Objetivos: Elevar los niveles de salud de la población realizando tratamientos que contemplen la eliminación de patologías orales y la posterior confección, prueba e instalación de prótesis completas y parciales de acrílico.Facultad de Odontologí