The Federal Trademark Dilution Act of 1995: Potent Weapon or Uphill Battle?

Following a brief discussion of the history of trademark infringement law, the events leading to the FTDA, and an overview of the FTDA, this paper discusses the major causes of the FTDA\u27s ineffectiveness. We will then review the application of the act, discuss its implications on the future of trademark ownership in business, and suggest improvements to the legal application of the act

Developing a competence framework for cognitive analytic therapy

Objective This paper describes the development and summarizes the content of a competence framework for delivery of cognitive analytic therapy (CAT). Design The framework was developed using the evidence‐based method developed by Roth and Pilling (2008, Behavioural and Cognitive Psychotherapy, 36, 129). Methods A review of the CAT outcome literature identified where CAT interventions had evidence of efficacy. Standard texts on CAT were primary sources for details of theory and practice. This process was supported by an expert reference group (ERG). The role of the ERG was to provide professional advice on areas where the evidence base was lacking, but where CAT interventions were commonly used by therapists trained in the model. Results A framework was produced and structured in terms of core knowledge, core skills, and meta‐competences (which require therapeutic judgement rather than simple adherence to a treatment protocol). Conclusions The framework enables trainees, service users, service managers, and commissioners to better understand a) the core features of CAT and b) what competences need to be in place for CAT to be skilfully delivered in practice. Practitioner points It is possible to define the core competences of CAT. Whilst generic competences are important, there are five CAT‐specific domains of competence. The CAT‐specific competences reflect the three‐phase structure of the therapy: reformulation, recognition, and revision

Working Toward a Post-Adoptive Model (PAM): Initial Steps for a Meta-Analysis of Post-Adoptive System Use

System use is a focal concept in information systems (IS) research in light of the fact that the benefits of information systems can only be realized when people use them after adoption. In the past decade, IS researchers have attempted to enhance the conceptualizations of system use from various perspectives. Nevertheless, our understanding of post-adoptive system use still remains at the early stage and scattered. In this research, we attempt to develop an initial post-adoptive model (PAM) based on early and preliminary steps toward a meta-analysis of the system use literature in the top IS journals. These initial steps give us a starting point for a full meta-analysis of this stream of research. Moving forward, we will conduct this analysis so as to develop a picture about the status quo of existing research on post-adoptive system use. Ultimately, our goals are to begin a synthesis of existing research models and point out directions for future research in the area of post-adoptive system use

Bipolar Nanosecond Electric Pulses are Less Efficient at Electropermeabilization and Killing Cells than Monopolar Pulses

Multiple studies have shown that bipolar (BP) electric pulses in the microsecond range are more effective at permeabilizing cells while maintaining similar cell survival rates as compared to monopolar (MP) pulse equivalents. In this paper, we investigated whether the same advantage existed for BP nanosecond-pulsed electric fields (nsPEF) as compared to MP nsPEF. To study permeabilization effectiveness, MP or BP pulses were delivered to single Chinese hamster ovary (CHO) cells and the response of three dyes, Calcium Green-1, propidium iodide (PI), and FM1-43, was measured by confocal microscopy. Results show that BP pulses were less effective at increasing intracellular calcium concentration or PI uptake and cause less membrane reorganization (FM1-43) than MP pulses. Twenty-four hour survival was measured in three cell lines (Jurkat, U937, CHO) and over ten times more BP pulses were required to induce death as compared to MP pulses of similar magnitude and duration. Flow cytometry analysis of CHO cells after exposure (at 15 min) revealed that to achieve positive FITC-Annexin V and PI expression, ten times more BP pulses were required than MP pulses. Overall, unlike longer pulse exposures, BP nsPEF exposures proved far less effective at both membrane permeabilization and cell killing than MP nsPEF

Two-phase equilibrium and molecular hydrogen formation in damped Lyman-alpha systems

Molecular hydrogen is quite underabundant in damped Lyman-alpha systems at high redshift, when compared to the interstellar medium near the Sun. This has been interpreted as implying that the gas in damped Lyman-alpha systems is warm. like the nearby neutral intercloud medium, rather than cool, as in the clouds which give rise to most H I absorption in the Milky Way. Other lines of evidence suggest that the gas in damped Lyman-alpha systems -- in whole or part -- is actually cool; spectroscopy of neutral and ionized carbon, discussed here, shows that the damped Lyman-alpha systems observed at lower redshift z $$ 2.8 are warm (though not devoid of H2). To interpret the observations of carbon and hydrogen we constructed detailed numerical models of H2 formation under the conditions of two-phase thermal equilibrium, like those which account for conditions near the Sun, but with varying metallicity, dust-gas ratio, $etc$. We find that the low metallicity of damped Lyman-alpha systems is enough to suppress H2 formation by many orders of magnitude even in cool diffuse clouds, as long as the ambient optical/uv radiation field is not too small. For very low metallicity and under the most diffuse conditions, H2 formation will be dominated by slow gas-phase processes not involving grains, and a minimum molecular fraction in the range $10^{-8}-10^{-7}$ is expected.Comment: 13 pages, 7 figures; accepted 2002-04-30 by Astronomy and Astrophysic

Dose-Dependent Thresholds of 10-ns Electric Pulse Induced Plasma Membrane Disruption and Cytotoxicity in Multiple Cell Lines

In this study, we determined the LD50 (50% lethal dose) for cell death, and the ED50 (50% of cell population staining positive) for propidium (Pr) iodide uptake, and phosphatidylserine (PS) externalization for several commonly studied cell lines (HeLa, Jurkat, U937, CHO-K1, and GH3) exposed to 10-ns electric pulses (EP). We found that the LD50 varied substantially across the cell lines studied, increasing from 51 J/g for Jurkat to 1861 J/g for HeLa. PS externalized at doses equal or lower than that required for death in all cell lines ranging from 51 J/g in Jurkat, to 199 J/g in CHO-K1. Pr uptake occurred at doses lower than required for death in three of the cell lines: 656 J/g for CHO-K1, 634 J/g for HeLa, and 142 J/g for GH3. Both Jurkat and U937 had a LD50 lower than the ED50 for Pr uptake at 780 J/g and 1274 J/g, respectively. The mechanism responsible for these differences was explored by evaluating cell size, calcium concentration in the exposure medium, and effect of trypsin treatment prior to exposure. None of the studied parameters correlated with the observed results suggesting that cellular susceptibility to injury and death by 10-ns EP was largely determined by cell physiology. In contrast to previous studies, our findings suggest that permeabilization of internal membranes may not necessarily be responsible for cell death by 10-ns EP. Additionally, a mixture of Jurkat and HeLa cells was exposed to 10-ns EP at a dose of 280 J/g. Death was observed only in Jurkat cells suggesting that 10-ns EP may selectively kill cells within a heterogeneous tissue

A metaproteomic approach to study human-microbial ecosystems at the mucosal luminal interface

Aberrant interactions between the host and the intestinal bacteria are thought to contribute to the pathogenesis of many digestive diseases. However, studying the complex ecosystem at the human mucosal-luminal interface (MLI) is challenging and requires an integrative systems biology approach. Therefore, we developed a novel method integrating lavage sampling of the human mucosal surface, high-throughput proteomics, and a unique suite of bioinformatic and statistical analyses. Shotgun proteomic analysis of secreted proteins recovered from the MLI confirmed the presence of both human and bacterial components. To profile the MLI metaproteome, we collected 205 mucosal lavage samples from 38 healthy subjects, and subjected them to high-throughput proteomics. The spectral data were subjected to a rigorous data processing pipeline to optimize suitability for quantitation and analysis, and then were evaluated using a set of biostatistical tools. Compared to the mucosal transcriptome, the MLI metaproteome was enriched for extracellular proteins involved in response to stimulus and immune system processes. Analysis of the metaproteome revealed significant individual-related as well as anatomic region-related (biogeographic) features. Quantitative shotgun proteomics established the identity and confirmed the biogeographic association of 49 proteins (including 3 functional protein networks) demarcating the proximal and distal colon. This robust and integrated proteomic approach is thus effective for identifying functional features of the human mucosal ecosystem, and a fresh understanding of the basic biology and disease processes at the MLI. © 2011 Li et al