The actin binding proteins cortactin and HS1 are dispensable for platelet actin nodule and megakaryocyte podosome formation

A dynamic, properly organised actin cytoskeleton is critical for the production and haemostatic function of platelets. The Wiskott Aldrich Syndrome protein (WASp) and Actin-Related Proteins 2 & 3 Complex (Arp2/3 complex) are critical mediators of actin polymerisation and organisation in many cell types. In platelets and megakaryocytes, these proteins have been shown to be important for proper platelet production and function. The cortactin family of proteins (Cttn & HS1) are known to regulate WASp-Arp2/3-mediated actin polymerisation in other cell types and so here we address the role of these proteins in platelets using knockout mouse models. We generated mice lacking Cttn and HS1 in the megakaryocyte/platelet lineage. These mice had normal platelet production, with platelet number, size and surface receptor profile comparable to controls. Platelet function was also unaffected by loss of Cttn/HS1 with no differences observed in a range of platelet function assays including aggregation, secretion, spreading, clot retraction or tyrosine phosphorylation. No effect on tail bleeding time or in thrombosis models was observed. In addition, platelet actin nodules, and megakaryocyte podosomes, actin-based structures known to be dependent on WASp and the Arp2/3 complex, formed normally. We conclude that despite the importance of WASp and the Arp2/3 complex in regulating F-actin dynamics in many cells types, the role of cortactin in their regulation appears to be fulfilled by other proteins in platelets

Determining Priority and Sustainable Strategies in Agribusiness Management using an Analytic Hierarchy Process model

Publisher Copyright: © 2023, ANPAD - Associacao Nacional de Pos-Graduacao e Pesquisa em Administracao. All rights reserved.The aim of this study was to analyze which business sustainability factors are adopted in the strategic process of the agricultural sector using the model named Strategic Planning for Business Sustainability (PEPSE). To achieve the proposed aim, the PEPSE model was applied to the Analytic Hierarchy Process multicriteria decision tool. During the research, the farm adopted sanitary measures due to the COVID-19 pandemic, which compromised access to managers and, consequently, data collection. Based on the application of a model developed especially for the identification and formulation of sustainable strategies, the study identified how sustainability is considered in the strategic planning of an agricultural unit in Brazil and the strategies adopted to deal with environmental variables. It was possible to understand how the stakeholders influence the planning of the farm and the variables and priority strategies for the environmental positioning of the farm. Thus, the main limitation of the research was the time and the collection of information, therefore, only an analysis of the external scenario of the farm was carried out.publishersversionpublishe

Avaliação dos impactos da Tecnologia Acasalamento de Outono em Bovinos de Corte.

Identificação da tecnologia; Procedimentos metodológicos; Identificação dos impactos na cadeia produtiva; Impactos econômicos; Impactos sociais; Impactos ambientais; Avaliação integrada e comparativa dos impactos gerados; Custos para a geração da tecnologia; Ações sociais.bitstream/item/64239/1/DT89.pd

Complementary stereotyping of ethnic minorities predicts system justification in Poland

We investigate the phenomenon of complementary stereotyping of ethnic minorities in Poland and its relationship to system justification. Using results from a nationally representative survey we test the hypothesis that complementary stereotypes—according to which ethnic minorities are seen as possessing distinctive, offsetting strengths and weaknesses—would be associated with system justification among Polish majority citizens. For four minorities, results indicated that stereotyping them as (a) low in morality but high in competence or (b) high in morality but low in competence predicted greater system justification. These results suggest that even in a context that is low in support for the status quo, complementary stereotyping of ethnic minorities is linked to system justification processes. For the three minority groups that were lowest in social status, complementary stereotyping was unrelated to system justification. It appears that negative attitude towards these groups can be expressed openly, regardless of one’s degree of system justification

The Effects of Sex-Role Attitudes and Group Composition on Men and Women in Groups

The dual impact of group gender composition and sex-role attitudes on self-perceptions and social behavior was explored. Androgynous and stereotyped men and women were placed in groups of skewed sex composition. Subjects\u27 self-descriptions of masculine attributes shifted significantly in the group environment. In some instances, sex role-stereotyped subjects responded most stereotypically when their gender was in the minority in the group. Differences between men and women and between androgynous and stereotyped subjects in sex role-related preferences for group roles and discussion topics were also found