INVESTIGATION OF ANTIHEMOLYTIC, XANTHINE OXIDASE INHIBITION, ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF SALVIA VERBENACA L. AERIAL PART EXTRACTS.

Background: In this study, Salvia verbenaca L. aerial part extracts (SVEs): were screened for their antihemolytic, xanthine oxidase (XO) inhibition, antioxidant and antimicrobial activities. Material and methods: To investigate SVEs antihemolytic activity, the 2,2,-azobis (2-amidinopropane) dihydrochloride (AAPH) was used to induce erythrocyte oxidative hemolysis. In XO inhibition test, xanthine was used as substrate and cytochrome c for generating superoxide anions. The antioxidant activity of SVEs was examined by means of reducing power, DPPH free radical scavenging and iron chelating assays. In addition, SVEs were tested for their antimicrobial effects by evaluating antibacterial and antifungal activities. Results: Ethyl acetate extract (EAE) contains the highest amount of total polyphenols and flavonoids (661.78 ± 4.00 mg GAE / g E) and (28.81 ± 0.38 mg QE / g E) respectively. In antihemolytic test EAE was the most active extract with an HT50 value of 165 min. SVEs gave significant inhibitory effects on XO, especially the chloroform extract (ChE) with IC50 value of 0.0088 ± 0.000 mg/ml. EAE was the most active extract in reducing power essay (EC50: 0.0047 ± 0.000 mg/ml) and in DPPH radical scavenging essay (IC50: 0.0086 ± 0.000 mg/ml). Finally, the EAE has inhibited the growth of nine bacterial strains with inhibition zone diameters of (12 to 16 mm), but no activities have found against fungal strains. Conclusion: S. verbenaca could be considered as a potential source of natural antihemolytic, enzyme modulator, antioxidant and antibacterial agents

Antioxidant and radical scavenging properties of Carthamus caeruleus L extracts grow wild in Algeria flora

Four sub-fractions of Carthamus caeruleus L (whichbelongs to the family Asteraceae) root extracts, i.e., methanol (CE), chloroform (CHE), ethyl acetate (EAE) and aqueous (AE) extracts were screened for their total phenolics, flavonoids and possible antioxidant activities, using DPPH free radical-scavenging and β-carotene/linoleic acid assays. The results indicated that total polyphenols were highest in EAE followed by CHE. However, the free radical scavenging activity of CHE was higher than the other extracts with an IC50 value of 53.26 µg/mL. Determination of quercetin, gallic acid and rutin showed antioxidant activity as positive controls in parallel experiments. Moreover, oxidation of linoleic acid was effectively inhibited by AE, EAE and CE which were too close to each other. Also, the CHE was more effective compared to the synthetic antioxidant BHT. It could be concluded that Carthamus caeruleus L growing wild in the Mediterranean regions and especially in Algeria has a potent antioxidant activity and could be evaluated as a starting point for further investigations.Four sub-fractions of Carthamus caeruleus L (which belongs to the family Asteraceae) root extracts,i.e., methanol (CE), chloroform (CHE), ethyl acetate (EAE) and aqueous (AE) extracts were screenedfor their total phenolics, flavonoids and possible antioxidant activities, using DPPH free radicalscavengingand β-carotene/linoleic acid assays. The results indicated that total polyphenols werehighest in EAE followed by CHE. However, the free radical scavenging activity of CHE was higherthan the other extracts with an IC50 value of 53.26 μg/mL. Determination of quercetin, gallic acid andrutin showed antioxidant activity as positive controls in parallel experiments. Moreover, oxidation oflinoleic acid was effectively inhibited by AE, EAE and CE which were too close to each other. Also,the CHE was more effective compared to the synthetic antioxidant BHT. It could be concluded thatCarthamus caeruleus L growing wild in the Mediterranean regions and especially in Algeria has apotent antioxidant activity and could be evaluated as a starting point for further investigations. Propriedades dos radicais antioxidantes extraídos de Carthamus caeruleus L nativosda flora da ArgéliaQuarto sub-frações extraídas da raiz de Carthamus caeruleus L (que pertencem à famíliaAsteraceae), i.e., metanol (CE), clorofórmio (CHE), acetato de etil (EAE) e extratos aquosos foramexaminados minuciosamente quanto aos seus fenólicos, flanóides e suas atividades antioxidantespossíveis, usando DPPH extração de radicais livres e B-caroteno/ácido linoléico. Os resultadosindicaram que os polifenóis totais foram maiores em EAE seguido de CHE. Entretanto, a atividadede extração de radicais livres em CHE foi maior que de outros extratos com um valor de IC50 de of5326 μg/mL. A determinação de quercetina ácido gálico e rutina demonstrou que a atividade deantioxidante funciona como controle positive em experimentos paralelos. Além disso, a oxidaçãode ácido lenoico foi efetivamente inibida por AE, EAE e CE que estavam muito próximos uns aosoutros. Também, o CHE foi mais eficaz se comparado ao antioxidante sintético BHT. Foi possívelconcluir que Carthamus caeruleus L nativos das nas regiões do Mediterrâneo, e, especialmente naArgélia, tem uma potente atividade antioxidante e poderia ser avaliada como o ponto de partidapara investigações futuras