Crystallization of Hevamine, an Enzyme with Lysozyme/Chitinase Activity from Hevea brasiliensis Latex

Hevamine, an enzyme with both lysozyme and chitinase activity, was isolated and purified from Hevea brasiliensis (rubber tree) latex. The enzyme (molecular weight 29,000) is homologous to certain “pathogenesis-related” proteins from plants, but not to hen egg-white or phage T4 lysozyme. To investigate the atomic details of the substrate specificity and the cause for hevamine’s low pH optimum (pH 4.0), we have crystallized two hevamine isozymes as a first step towards a high-resolution X-ray structure determination. Suitable crystals were obtained at room temperature from hanging drop experiments by vapor diffusion against 1.7 M to 3.4 M-NaCl (pH 5.0 to 9.0) for the major isozyme, and by vapor diffusion against 2.5 M to 4.3 M-NaCl (pH 5.0 to 8.0) for the minor one. Both isozymes give the same crystal morphology and space group. Their space group is P212121 with cell dimensions a = 82.3 Å, b = 58.1 Å and c = 52.5 Å (1 Å = 0.1 nm). The crystals diffract to at least 2.0 Å resolution

Properties of Dust Grains in Planetary Nebulae -- I. The Ionized Region of NGC 6445

In this paper we study new infrared spectra of the evolved planetary nebula NGC 6445 obtained with ISO. These data show that the thermal emission from the grains is very cool and has a low flux compared to H beta. A model of the ionized region is constructed, using the photo-ionization code CLOUDY 90.05. Based on this model, we show from depletions in the gas phase elements that little grain destruction can have occurred in the ionized region of NGC 6445. We also argue that dust-gas separation in the nebula is not plausible. The most likely conclusion is that grains are residing inside the ionized region of NGC 6445 and that the low temperature and flux of the grain emission are caused by the low luminosity of the central star and the low optical depth of the grains. This implies that the bulk of the silicon-bearing grains in this nebula were able to survive exposure to hard UV photons for at least several thousands of years, contradicting previously published results. A comparison between optical and infrared diagnostic line ratios gives a marginal indication for the presence of a t^2-effect in the nebula. However, the evidence is not convincing and other explanations for the discrepancy are also plausible. The off-source spectrum taken with ISO-LWS clearly shows the presence of a warm cirrus component with a temperature of 24 K as well as a very cold component with a temperature of 7 K. Since our observation encompasses only a small region of the sky, it is not clear how extended the 7 K component is and whether it contributed significantly to the FIRAS spectrum taken by COBE. Because our line of sight is in the galactic plane, the very cold component could be a starless core.Comment: 36 pages, 8 tables, 7 figures, accepted for publication in Ap

The Evolution of NGC 7027 at Radio Frequencies: A New Determination of the Distance and Core Mass

We present the results of a 25-year program to monitor the radio flux evolution of the planetary nebula NGC7027. We find significant evolution of the spectral flux densities. The flux density at 1465 MHz, where the nebula is optically thick, is increasing at a rate of 0.251+-0.015 % per year, caused by the expansion of the ionized nebula. At frequencies where the emission is optically thin, the spectral flux density is changing at a rate of -0.145+-0.005 % per year, caused by a decrease in the number of ionizing photons coming from the central star. A distance of 980+-100 pc is derived. By fitting interpolated models of post-AGB evolution to the observed changes, we find that over the 25-yr monitoring period, the stellar temperature has increased by 3900+-900 K and the stellar bolometric luminosity has decreased by 1.75+-0.38 %. We derive a distance-independent stellar mass of 0.655+-0.01 solar masses adopting the Bloecker stellar evolution models, or about 0.04 solar masses higher when using models of Vassiliadis & Wood which may provide a better fit. A Cloudy photoionization model is used to fit all epochs at all frequencies simultaneously. The differences between the radio flux density predictions and the observed values show some time-independent residuals of typically 1 %. A possible explanation is inaccuracies in the radio flux scale of Baars et al. We propose an adjustment to the flux density scale of the primary radio flux calibrator 3C286, based on the Cloudy model of NGC7027. We also calculate precise flux densities for NGC7027 for all standard continuum bands used at the VLA, as well as for some new 30GHz experiments.Comment: submitted to the Astrophysical Journa

Design and status of the detector block for the ISO-SWS

The Short Wave Spectrometer (SWS) is one of the two spectrometers for the Infrared Space Observatory (ISO). It consists of a pair of grating spectrometers and a Fabry-Perot interferometer. Together, the grating spectrometers cover the wavelength range 2.4 to 45 microns, at a resolution between 1000 and 2000. The Fabry-Perot interferometer, in series with one of the grating spectrometers, provides a resolution of about 20,000 at the wavelengths between 15 and 35 microns. The SWS is being built by the Space Research Organization of the Netherlands and the Max Planck Institute for Extraterrestrial Physics in Garching, Germany. The spectrometer has 52 discrete detectors, most of which are bulk detectors. In the design of the spectrometer, the main emphasis is on the sensitivity of the individual channels, rather than on the number of detectors. This was one of the main reasons to select non-destructive read-out circuits, with a separate heated-JFET pre-amplifier for each individual detector. The signals are amplified and filtered in parallel. The engineering tests on the SWS detector block have not yet been completed. The design of the detector block is described and the present problem areas are indicated

The 5.25 & 5.7 μ\mum Astronomical Polycyclic Aromatic Hydrocarbon Emission Features

Astronomical mid-IR spectra show two minor PAH features at 5.25 and 5.7 $\mu$m (1905 and 1754 cm$^{\rm - 1}$) that hitherto have been little studied, but contain information about the astronomical PAH population that complements that of the major emission bands. Here we report a study involving both laboratory and theoretical analysis of the fundamentals of PAH spectroscopy that produce features in this region and use these to analyze the astronomical spectra. The ISO SWS spectra of fifteen objects showing these PAH features were considered for this study, of which four have sufficient S/N between 5 and 6 $\mu$m to allow for an in-depth analysis. All four astronomical spectra show similar peak positions and profiles. The 5.25 $\mu$m feature is peaked and asymmetric, while the 5.7 $\mu$m feature is broader and flatter. Detailed analysis of the laboratory spectra and quantum chemical calculations show that the astronomical 5.25 and 5.7 $\mu$m bands are a blend of combination, difference and overtone bands primarily involving CH stretching and CH in-plane and CH out-of-plane bending fundamental vibrations. The experimental and computational spectra show that, of all the hydrogen adjacency classes possible on PAHs, solo and duo hydrogens consistently produce prominent bands at the observed positions whereas quartet hydrogens do not. In all, this a study supports the picture that astronomical PAHs are large with compact, regular structures. From the coupling with primarily strong CH out-of-plane bending modes one might surmise that the 5.25 and 5.7 $\mu$m bands track the neutral PAH population. However, theory suggests the role of charge in these astronomical bands might also be important.Comment: Accepted ApJ, 40 pages in pre-print, 14 figures, two onlin