First record of Temnosewellia minor (Platyhelminthes, Temnocephalidae) in Sicily, with a plea for a re-examination of the identity of the publicly available molecular sequences of the genus

Ectosymbiotic temnocephalan flatworms belonging to the genus Temnosewellia were collected on Cherax destructor in an aquaculture farm in Sicily, Italy. This represents the first record of a temnocephalan species for the fauna of the island. Morphological and molecular identification of the collected specimens proved that they belong to the allochthonous species Temnosewellia minor, which was introduced along with crayfishes bred in aquaculture farms. The phylogenetic analyses carried out for the molecular identification of the Sicilian population highlighted some inconsistencies in the grouping of the Temnosewellia sequences available online, thus stressing the opportunity of a careful re-examination of the voucher samples and their identifications. The risks of its unwary introduction in the wild and the need of monitoring its possible impacts on native biota are briefly discussed

Patterns of genomic instability in gastric cancer: clinical implications and perspectives.

In gastric cancer (GC) the loss of genomic stability represents a key molecular step that occurs early in the carcinogenesis process and creates a permissive environment for the accumulation of genetic and epigenetic alterations in tumor suppressor genes and oncogenes. It is widely accepted that GC can follow at least two major genomic instability pathways, microsatellite instability (MSI) and chromosome instability (CIN). MSI is responsible for a well-defined subset of GCs. CIN represents a more common pathway comprising heterogeneous subsets of GC. In addition to MSI and CIN, the CpG islands methylator phenotype (CIMP) plays an important role in gastric carcinogenesis. CIMP may lead to the transcriptional silencing of various genes in gastric carcinogenesis. Intriguingly, more recently in addition to CpG island hypermethylation, a global DNA demethylation, that precedes genomic damage, has been observed in GC. Thus, epigenetic alterations may play a relevant role in gastric carcinogenesis as alternative mechanisms. Evidence suggests that although MSI, CIN and CIMP phenotypes can be distinguished from one another, there might be some degree of overlap. This review describes our current knowledge of the instability pathways in gastric carcinogenesis and the potential clinical applications for different forms of genomic instability in GC

Docosahexaenoic Acid Therapy of Experimental Ischemic Stroke

We examined the neuroprotective efficacy of docosahexaenoic acid (DHA), an omega-3 essential fatty acid family member, in acute ischemic stroke; studied the therapeutic window; and investigated whether DHA administration after an ischemic stroke is able to salvage the penumbra. In each series described below, SD rats underwent 2 h of middle cerebral artery occlusion (MCAo). In series 1, DHA or saline was administered i.v. at 3, 4, 5, or 6 h after stroke. In series 2, MRI was conducted on days 1, 3 and 7. In series 3, DHA or saline was administered at 3 h, and lipidomic analysis was conducted on day 3. Treatment with DHA significantly improved behavior and reduced total infarct volume by a mean of 40% when administered at 3 h, by 66% at 4 h, and by 59% at 5 h. Total lesion volumes computed from T2-weighted images were reduced in the DHA group at all time points. Lipidomic analysis showed that DHA treatment potentiates neuroprotectin D1 (NPD1) synthesis in the penumbra 3 days after MCAo. DHA administration provides neurobehavioral recovery, reduces brain infarction and edema, and activates NPD1 synthesis in the penumbra when administered up to 5 h after focal cerebral ischemia in rats

Two-Dimensional Hydrodynamics of Pre-Core Collapse: Oxygen Shell Burning

By direct hydrodynamic simulation, using the Piecewise Parabolic Method (PPM) code PROMETHEUS, we study the properties of a convective oxygen burning shell in a SN 1987A progenitor star prior to collapse. The convection is too heterogeneous and dynamic to be well approximated by one-dimensional diffusion-like algorithms which have previously been used for this epoch. Qualitatively new phenomena are seen. The simulations are two-dimensional, with good resolution in radius and angle, and use a large (90-degree) slice centered at the equator. The microphysics and the initial model were carefully treated. Many of the qualitative features of previous multi-dimensional simulations of convection are seen, including large kinetic and acoustic energy fluxes, which are not accounted for by mixing length theory. Small but significant amounts of carbon-12 are mixed non-uniformly into the oxygen burning convection zone, resulting in hot spots of nuclear energy production which are more than an order of magnitude more energetic than the oxygen flame itself. Density perturbations (up to 8%) occur at the `edges' of the convective zone and are the result of gravity waves generated by interaction of penetrating flows into the stable region. Perturbations of temperature and electron fraction at the base of the convective zone are of sufficient magnitude to create angular inhomogeneities in explosive nucleosynthesis products, and need to be included in quantitative estimates of yields. Combined with the plume-like velocity structure arising from convection, the perturbations will contribute to the mixing of nickel-56 throughout supernovae envelopes. Runs of different resolution, and angular extent, were performed to test the robustness of theseComment: For mpeg movies of these simulations, see http://www.astrophysics.arizona.edu/movies.html Submitted to the Astrophysical Journa

Axisymmetric core collapse simulations using characteristic numerical relativity

We present results from axisymmetric stellar core collapse simulations in general relativity. Our hydrodynamics code has proved robust and accurate enough to allow for a detailed analysis of the global dynamics of the collapse. Contrary to traditional approaches based on the 3+1 formulation of the gravitational field equations, our framework uses a foliation based on a family of outgoing light cones, emanating from a regular center, and terminating at future null infinity. Such a coordinate system is well adapted to the study of interesting dynamical spacetimes in relativistic astrophysics such as stellar core collapse and neutron star formation. Perhaps most importantly this procedure allows for the unambiguous extraction of gravitational waves at future null infinity without any approximation, along with the commonly used quadrupole formalism for the gravitational wave extraction. Our results concerning the gravitational wave signals show noticeable disagreement when those are extracted by computing the Bondi news at future null infinity on the one hand and by using the quadrupole formula on the other hand. We have strong indication that for our setup the quadrupole formula on the null cone does not lead to physical gravitational wave signals. The Bondi gravitational wave signals extracted at infinity show typical oscillation frequencies of about 0.5 kHz.Comment: 17 pages, 18 figures, submitted to Phys. Rev.

Hadron Energy Reconstruction for the ATLAS Calorimetry in the Framework of the Non-parametrical Method

This paper discusses hadron energy reconstruction for the ATLAS barrel prototype combined calorimeter (consisting of a lead-liquid argon electromagnetic part and an iron-scintillator hadronic part) in the framework of the non-parametrical method. The non-parametrical method utilizes only the known $e/h$ ratios and the electron calibration constants and does not require the determination of any parameters by a minimization technique. Thus, this technique lends itself to an easy use in a first level trigger. The reconstructed mean values of the hadron energies are within $\pm 1$ of the true values and the fractional energy resolution is $[(58\pm3)/\sqrt{E}+(2.5\pm0.3)$. The value of the $e/h$ ratio obtained for the electromagnetic compartment of the combined calorimeter is $1.74\pm0.04$ and agrees with the prediction that $e/h > 1.7$ for this electromagnetic calorimeter. Results of a study of the longitudinal hadronic shower development are also presented. The data have been taken in the H8 beam line of the CERN SPS using pions of energies from 10 to 300 GeV.Comment: 33 pages, 13 figures, Will be published in NIM

New national and regional Annex I Habitat records: From #37 to #44

In this contribution, Italian new data concerning the distribution of the Annex I Habitats 3150, 3170*, 3260, 4090, 91L0, 91M0, 9340 are reported. In detail, 20 new occurrences in Natura 2000 sites are presented and 30 new cells are added in the EEA 10 km × 10 km reference grid. The new data refer to the Italian administrative regions of Campania, Lazio, Sardinia, Sicily, Tuscany, and Umbria

Heterogeneity of Dengue Illness in Community-Based Prospective Study, Iquitos, Peru

Measuring heterogeneity of dengue illness is necessary to define suitable endpoints in dengue vaccine and therapeutic trials and will help clarify behavioral responses to illness. To quantify heterogeneity in dengue illness, including milder cases, we developed the Dengue Illness Perceptions Response (IPR) survey, which captured detailed symptom data, including intensity, duration, and character, and change in routine activities caused by illness. During 2016–2019, we collected IPR data daily during the acute phase of illness for 79 persons with a positive reverse transcription PCR result for dengue virus RNA. Most participants had mild ambulatory disease. However, we measured substantial heterogeneity in illness experience, symptom duration, and maximum reported intensity of individual symptoms. Symptom intensity was a more valuable predicter of major activity change during dengue illness than symptom presence or absence alone. These data suggest that the IPR measures clinically useful heterogeneity in dengue illness experience and its relation to altered human behavior