Imide and isatin derivatives as β-lactam mimics of β-lactam antibiotics

Activated γ-lactams, which are derivatives of succinimide, phthalimide and isatin with suitable elements of molecular recognition, have been synthesised as mimics of the ß-lactam antibiotics and their chemical and biological reactivity determined

Secondary metabolite profiling of the model legume Lotus japonicus during its symbiotic interaction with Mesorhizobium loti

Plant secondary metabolites, particularly flavonoids, are key components in the early stages of nitrogen-fixing symbiosis. Despite their importance, the endogenous secondary metabolites involved in symbiosis have not yet been identified in the model legume Lotus japonicus. We therefore determined changes in the secondary metabolic profile of Lotus japonicus roots in response to its symbiont. Analysis of the root secondary metabolite profiles 1 week after inoculation with Mesorhizobium loti revealed quantitative changes in the level of 14 phenolic peaks when compared with non-inoculated control plants. These changes affected compounds from most phenolic classes, possibly resulting from interconversion between classes since the total phenolic level remained constant. In addition, the use of 2 M. loti strains differing only in their capacity to synthesise Nod factor revealed that, although Nod factor signalling induced accumulation of a specific subset of 4 phenolic peaks, most changes were induced in response to both rhizobial strains.NR was supported by a Training Network funded by the European Training Network EU-RTN-LOTUS-HPRN-CT-2000-00086. KJW was supported by core funding from the Biotechnology and Biological Science Research Council (BBSRC), UK.Peer Reviewe

Fragmentation and systematics of the Pygmy Dipole Resonance in the stable N=82 isotones

The low-lying electric dipole (E1) strength in the semi-magic nucleus 136Xe has been measured which finalizes the systematic survey to investigate the so-called pygmy dipole resonance (PDR) in all stable even N=82 isotones with the method of nuclear resonance fluorescence using real photons in the entrance channel. In all cases, a fragmented resonance-like structure of E1 strength is observed in the energy region 5 MeV to 8 MeV. An analysis of the fragmentation of the strength reveals that the degree of fragmentation decreases towards the proton-deficient isotones while the total integrated strength increases indicating a dependence of the total strength on the neutron-to-proton ratio. The experimental results are compared to microscopic calculations within the quasi-particle phonon model (QPM). The calculation includes complex configurations of up to three phonons and is able to reproduce also the fragmentation of the E1 strength which allows to draw conclusions on the damping of the PDR. Calculations and experimental data are in good agreement in the degree of fragmentation and also in the integrated strength if the sensitivity limit of the experiments is taken into account

Evolution of level density step structures from 56,57-Fe to 96,97-Mo

Level densities have been extracted from primary gamma spectra for 56,57-Fe and 96,97-Mo nuclei using (3-He,alpha gamma) and (3-He,3-He') reactions on 57-Fe and 97-Mo targets. The level density curves reveal step structures above the pairing gap due to the breaking of nucleon Cooper pairs. The location of the step structures in energy and their shapes arise from the interplay between single-particle energies and seniority-conserving and seniority-non-conserving interactions.Comment: 9 pages, including 5 figure

Tailoring of the Tell-us Card communication tool for nurses to increase patient participation using Intervention Mapping

Aims and objectives: To describe the tailoring of the Tell-us Card intervention for enhanced patient participation to the Dutch hospital setting using Intervention Mapping as a systematic approach. Background: Even though patient participation is essential in any patient-to-nurse encounter, care plans often fail to take patients' preferences into account. The Tell-us Card intervention seems promising, but needs to be tailored and tested before implementation in a different setting or on large scale. Design: Description of the Intervention Mapping framework to systematically tailor the Tell-us Card intervention to the Dutch hospital setting. Methods: Intervention Mapping consists of: (i) identification of the problem through needs assessment and determination of fit, based on patients and nurses interviews and focus group interviews; (ii) developing a logic model of change and matrices, based on literature and interviews; (iii) selection of theory-based methods and practical applications; (iv) producing programme components and piloting; (v) planning for adoption, implementation and sustainability; and (vi) preparing for programme evaluation. Results: Knowledge, attitude, outcome expectations, self-efficacy and skills were identified as the main determinants influencing the use of the Tell-us Card. Linking identified determinants and performance objectives with behaviour change techniques from the literature resulted in a well-defined and tailored intervention and evaluation plan. Conclusions: The Tell-us Card intervention was adapted to fit the Dutch hospital setting and prepared for evaluation. The Medical Research Council framework was followed, and the Intervention Mapping approach was used to prepare a pilot study to confirm feasibility and relevant outcomes. Relevance to clinical practice: This article shows how Intervention Mapping is applied within the Medical Research Council framework to adapt the Tell-us Card intervention, which could serve as a guide for the tailoring of similar interventions

Benzo[a]Pyrene Diol Epoxide Perturbation of Cell Cycle Kinetics of Synchronized Mouse Liver Epithelial Cells

A cell cycle synchronization system is described for the analysis of the perturbation of cell cycle kinetics and the cycle-phase specificity of chemicals and other agents. We used the system to study the effects of ({+-})r-7, t-8-dihydroxy-t-9, 10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BaP diol epoxide) upon the cell cycle of mouse liver epithelial cells(NMuLi). BaP diol epoxide(0.6 uM) was added to replated cultures of NMuLi cells that had been synchronized in various stages of the cell cycle by centrifugal elutriation. DNA histograms were obtained by flow cytometry as a function of time after replating. The data were analyzed by a computer modeling routine and reduced to a few graphs illustrating the 'net effects' of the BaP diol epoxide relative to controls. BaP diol epoxide slowed S-phase traversal in all samples relative to their respective control. Traversal through G{sub 2}M was also slowed by at least 50%. BaP diol epoxide had no apparent effect upon G{sub 1} traversal by cycling cells, but delayed the recruitment of quiescent G{sub 0} cells by about 2 hrs. The methods described constitute a powerful new approach for probing the cell cycle effects of a wide variety of agents. The present system appears to be extremely sensitive and capable of characterizing the action of agents on each phase of the cell cycle. The methods are automatable and would allow for the assay and possible differential characterization of mutagens and carcinogens

Critical fluctuations in superconductors and the magnetic field penetration depth

The superconducting transition is studied within the one-loop renormalization group in fixed dimension $D=3$ and at the critical point. A tricritical behavior is found, and for $\kappa > \kappa_c$, an attractive charged fixed point, distinct from that of a neutral superfluid. The critical exponents of the continuous transition are evaluated, and it is shown that the anomalous dimension of the gauge field equals unity. This implies the proportionality of the magnetic field penetration depth and the superconducting correlation length below the transition. The penetration depth exponent is nonclassical. We argue that it can not be extracted from the dual theory in a straightforward manner since it is not renormalized by fluctuations of the dual field.Comment: 12 pages, LaTex, two figures available upon reques

Masses and Phase Structure in the Ginzburg-Landau Model

We study numerically the phase structure of the Ginzburg-Landau model, with particular emphasis on mass measurements. There is no local gauge invariant order parameter, but we find that there is a phase transition characterized by a vanishing photon mass. For type I superconductors the transition is of 1st order. For type II 1st order is excluded by susceptibility analysis, but the photon correlation length suggests 2nd order critical behaviour with \nu ~ 1/2. The scalar mass, in contrast, does not show clear critical behaviour in the type II regime for V \to \infty, contrary to the conventional picture.Comment: 16 pages, 6 figures. More data gathered, allowing more definite conclusion

Dual superfluid-Bose glass critical point in two dimensions and the universal conductivity

We study the continuum version of the dual theory for a system of two-dimensional, zero temperature, disordered bosons, interacting with short range repulsion and at a commensurate density. The dual theory, which describes vortices in the bosonic ground state, and has a form of 3D classical scalar electrodynamics in random, correlated magnetic field, admits a new disordered critical point within RG calculation at fixed dimension. The universal conductivity and the critical exponents at the superfluid-Bose glass critical point are calculated as series in fixed-point values of the dual coupling constants, to the lowest non-trivial order: $\sigma_c = 0.25 (2e)^2 /h$, $\nu=1.38$ and $z=1.93$. The comparison with numerical results and experiments is discussed.Comment: 8 pages, LaTex, some clarifications and references adde