Genetic parameters for growth, reproductive and maternal traits in a multibreed meat sheep population

The genetic parameters for growth, reproductive and maternal traits in a multibreed meat sheep population were estimated by applying the Average Information Restricted Maximum Likelihood method to an animal model. Data from a flock supported by the Programa de Melhoramento Genético de Caprinos e Ovinos de Corte (GENECOC) were used. The traits studied included birth weight (BW), weaning weight (WW), slaughter weight (SW), yearling weight (YW), weight gain from birth to weaning (GBW), weight gain from weaning to slaughter (GWS), weight gain from weaning to yearling (GWY), age at first lambing (AFL), lambing interval (LI), gestation length (GL), lambing date (LD - number of days between the start of breeding season and lambing), litter weight at birth (LWB) and litter weight at weaning (LWW). The direct heritabilities were 0.35, 0.81, 0.65, 0.49, 0.20, 0.15 and 0.39 for BW, WW, SW, YW, GBW, GWS and GWY, respectively, and 0.04, 0.06, 0.10, 0.05, 0.15 and 0.11 for AFL, LI, GL, LD, LWB and LWW, respectively. Positive genetic correlations were observed among body weights. In contrast, there was a negative genetic correlation between GBW and GWS (-0.49) and GBW and GWY (-0.56). Positive genetic correlations were observed between AFL and LI, LI and GL, and LWB and LWW. These results indicate a strong maternal influence in this herd and the presence of sufficient genetic variation to allow mass selection for growth traits. Additive effects were of little importance for reproductive traits, and other strategies are necessary to improve the performance of these animals

Assistência domiciliar a idosos: fatores associados, características do acesso e do cuidado

OBJETIVO: Avaliar fatores associados à assistência domiciliar recebida pela população idosa e suas características, segundo modelos de atenção Estratégia Saúde da Família e modelo tradicional. MÉTODOS: Estudo transversal de base populacional, com amostra representativa de 1.593 indivíduos com 60 anos ou mais, residentes na região urbana de Bagé, RS, em 2008. A amostragem foi realizada em múltiplos estágios. Os dados foram coletados em entrevistas individuais. Foram analisadas as formas de acesso aos serviços, participação dos profissionais, satisfação e situação de saúde dos usuários após o atendimento. Foi utilizado modelo de regressão de Poisson para estimar as razões de prevalência bruta e ajustada, os respectivos intervalos com 95% de confiança e p-valor (teste de Wald). RESULTADOS: Assistência domiciliar foi estatisticamente associada à história prévia de acidente vascular cerebral, à presença de sinais de demência e à incapacidade para as atividades da vida diária. A família foi responsável por 75% das solicitações de cuidado. Nas áreas da atenção tradicional, os médicos responderam pela maior promoção de cuidados, enquanto nas áreas da Estratégia Saúde da Família destacou-se a participação da equipe de enfermagem. Aproximadamente 78% das solicitações foram atendidas em até 24 horas e 95% dos usuários avaliaram positivamente o cuidado recebido. Dois terços dos idosos referiram melhora nas condições de saúde. CONCLUSÕES: As variáveis associadas ao recebimento de assistência domiciliar reiteram os indicadores de fragilidade destacados na Política Nacional de Saúde da Pessoa Idosa e fortalecem a importância da estratégia na promoção da eqüidade no cuidado dos idosos. A avaliação positiva e o impacto na situação de saúde afirmam o domicílio como ambiente terapêutico.OBJECTIVE: To assess factors associated with home health care for the elderly and its characteristics based on different care models, the Family Health Strategy and traditional primary care. METHODS: A population-based cross-sectional study was conducted in a representative sample of 1,593 individuals aged >;60 years living in the urban area of the city of Bagé, Southern Brazil, in 2008. A multistage sampling was carried out. Data was collected during individual interviews about access to services, providers\u27 involvement, users\u27 satisfaction and health status after care. Poisson regression model was used for estimating crude and adjusted prevalence ratios, their related 95% confidence intervals and p-values (Wald test). RESULTS: Home health care was statistically associated with prior history of stroke, signs of dementia and disability in activities of daily living. The family was requested 75% of home care visits. Medical doctors provided most of the care in traditional primary care settings while nursing staff provided most care within the Family Health Strategy. Approximately 78% of the elderly received care within 24 hours after the request and 95% of them positively evaluated the care received. Two thirds of the elderly reported improved health status. CONCLUSIONS: The variables associated with home health care were consistent with fragility indicators included in the Brazilian National Health Policy for the Elderly, reinforcing the role of this strategy for promoting equitable health care to elderly population. Users\u27 satisfaction and the positive impact on their health status confirm home as a setting for providing care.OBJETIVO: Evaluar los factores asociados a la asistencia domiciliaria recibida por la población anciana y sus características, según modelos de atención Estrategia Salud de la Familia y modelo tradicional. MÉTODOS: Estudio transversal de base poblacional, con muestra representativa de 1.593 individuos con 60 años o más, residentes en la región urbana de Bagé, Sur de Brasil, en 2008. El muestreo fue realizado en múltiples fases. Los datos fueron colectados en entrevistas individuales. Se analizaron las formas de acceso a los servicios, participación de los profesionales, satisfacción y situación de salud de los usuarios posterior a la atención. Fue utilizado modelo de regresión de Poisson para estimar las tasas de prevalencia bruta y ajustada, los respectivos intervalos con 95% de confianza y p-valor (prueba de Wald). RESULTADOS: Asistencia domiciliaria fue estadísticamente asociada a la historia previa de enfermedad vascular cerebral, a la presencia de señales de demencia y a la incapacidad para las actividades de la vida diaria. La familia fue responsable por 75% de las solicitudes de cuidado. En las áreas de la atención tradicional, los médicos respondieron por la mayor promoción de cuidados, mientras que en las áreas de la Estrategia Salud de la Familia, se destacó la participación del equipo de enfermeras. Aproximadamente 78% de las solicitudes fueron atendidas en hasta 24 horas y 95% de los usuarios evaluaron positivamente el cuidado recibido. Dos tercios de los ancianos refirieron mejoría en las condiciones de salud. CONCLUSIONES: Las variables asociadas al recibimiento de asistencia domiciliaria reiteran los indicadores de fragilidad destacados en la Política Nacional de Salud de la Persona Anciana y fortalece la importancia de la estrategia en la promoción de la equidad en el cuidado de los ancianos. La evaluación positiva y el impacto en la situación de salud refuerzan el domicilio como ambiente terapéutico

Functional genomics of the horn fly, Haematobia irritans (Linnaeus, 1758)

<p>Abstract</p> <p>Background</p> <p>The horn fly, <it>Haematobia irritans </it>(Linnaeus, 1758) (Diptera: Muscidae) is one of the most important ectoparasites of pastured cattle. Horn flies infestations reduce cattle weight gain and milk production. Additionally, horn flies are mechanical vectors of different pathogens that cause disease in cattle. The aim of this study was to conduct a functional genomics study in female horn flies using Expressed Sequence Tags (EST) analysis and RNA interference (RNAi).</p> <p>Results</p> <p>A cDNA library was made from whole abdominal tissues collected from partially fed adult female horn flies. High quality horn fly ESTs (2,160) were sequenced and assembled into 992 unigenes (178 contigs and 814 singlets) representing molecular functions such as serine proteases, cell metabolism, mitochondrial function, transcription and translation, transport, chromatin structure, vitellogenesis, cytoskeleton, DNA replication, cell response to stress and infection, cell proliferation and cell-cell interactions, intracellular trafficking and secretion, and development. Functional analyses were conducted using RNAi for the first time in horn flies. Gene knockdown by RNAi resulted in higher horn fly mortality (protease inhibitor functional group), reduced oviposition (vitellogenin, ferritin and vATPase groups) or both (immune response and 5'-NUC groups) when compared to controls. Silencing of ubiquitination ESTs did not affect horn fly mortality and ovisposition while gene knockdown in the ferritin and vATPse functional groups reduced mortality when compared to controls.</p> <p>Conclusions</p> <p>These results advanced the molecular characterization of this important ectoparasite and suggested candidate protective antigens for the development of vaccines for the control of horn fly infestations.</p