7 research outputs found
Detection of Salmonella by Surface Plasmon Resonance
This study explores the possibility of simultaneous and specific detection of
Salmonella serovars by surface plasmon resonance (SPR). The Plasmonic® SPR device was
used to develop this rapid assay. The sandwich immunoassay involves the use of a
polyclonal anti-Salmonella antibody to simultaneous capture multiple Salmonella serovars
present in a sample. This is followed by specific detection of the captured serovars using
O-specific anti-Salmonella antibodies. Milk spiked with Salmonella Typhimurium and
Salmonella Enteritidis was used as a model system to establish the assay. The assay was
further extended to sequentially differentiate between the two Salmonella serovars on a
single SPR chip in a single channel. The assay was proved to work without any additional
dilution or clean-up steps. The sample volume requirement for the assay is only 10 ÎĽL. The
lower limits of detection for Salmonella Typhimurium and Salmonella Enteritidis were
2.50Ă—105 cells mL-1 and 2.50Ă—108 cells mL-1, respectively