PHYSIOCHEMICAL, PROXIMATE, AND SENSORY PROPERTIES OF UNFERMENTED AND FERMENTED SOY-CARROT BEVERAGES SWEETENED WITH SUGAR, DATE, AND HONEY

Objective: Physiochemical, proximate, and sensory properties of unfermented and fermented soy-carrot beverage sweetened with sugar, date, and honey were evaluated. Phytochemical content of soymilk, carrot juice, and their blend was also analyzed. Methods: Three sets of soy-carrot beverages were produced by homogenizing soy milk and carrot juice in a ratio of 2:1 and sweetened to 12% Brix. Each set was sweetened with sugar, date, and honey, respectively. A fourth set was unsweetened and served as control. After pasteurization, one part was fermented with pure culture of Lactobacillus acidophilus at 42°C for 24 h. Results: Fermentation significantly (p≤0.05) decreased pH (≥5.40–≤3.90), increased titratable acidity (≤0.55–≥0.90% lactic acid), and viscosity (≤0.65–≥0.87 Pa.S) of the soy-carrot beverages. Moisture, protein, fat, ash, carbohydrate, and energy content of unfermented beverages were 82.95– 93.95%, 2.15–2.87%, 0.42–1.21%, 0.10–0.20%, 3.21–12.55%, and 25.46–73.53 Kcal/g, respectively, while fermented beverages had 90.00–93.00%, 2.06–2.20%, 0.88–1.08%, 0.11–10.20%, 4.85–8.75%, and 36.76–52.20 Kcal/g, respectively. Total carotenoid, phenol, and DPPH radical scavenging activity varied, respectively, from 2.40–7.90, 14.81–26.59 mg tannic acid/ml, and 4.02–27.83% and were significantly (p≤0.05) highest in soy-carrot blend with carrot as major contributor. Degree of likeness of the sensory attributes for the sweetened and unfermented beverages was significantly (p≤0.05) higher than the fermented. Conclusion: Date and honey (12% Brix) can be used as sucrose alternatives in producing acceptable nutritious beverage from soymilk and carrot juice

PHYSICO-CHEMICAL, PROXIMATE COMPOSITION, ASCORBIC ACID, SENSORY, AND MICROBIOLOGICAL QUALITY OF MINIMALLY PROCESSED CARICA PAPAYA CONSUMED IN RIVERS STATE, NIGERIA

Objective: This study evaluated the physico-chemical, proximate, ascorbic acid, sensory, and microbiological properties of minimally processed Carica papaya consumed in Rivers State Nigeria. Methods: Minimally processed papaya in transparent polyethylene bags were purchased from four different locations: Nwinpi, Mile III, Rumuokuta, and Rumuokoro Junctions in Port Harcourt, Rivers State, Nigeria. Control sample was prepared in the laboratory. Standard analytical methods were used for analysis. Results: pH and titratable acidity ranged from 4.90–5.20 to 1.00–1.04% citric acid, respectively. Moisture, fat, ash, crude fiber, and carbohydrate ranged, respectively, from 85.80–89.60, 0.64–0.69, 0.55–0.96, 1.71–1.93, and 7.20–10.97%. Energy value was 35.31–50.07 kcal/g while protein was 0.09% for all samples. Ascorbic acid varied significantly (p<0.05) from 17.81 to 44.91 mg/100 g. Sensory results showed that 75% of the assessors’ degree of likeness for aroma, appearance/color, texture (smoothness), sweetness, and overall acceptability was that of moderate to extreme likeness. Total aerobic, coliform, Escherichia coli, Salmonella, and Staphylococcus aureus counts varied from 3.85–5.76, 3.74–5.68, 3.95–5.57, 3.82–5.58, and 3.30–5.45 Log10CFU/g, respectively. The control had significantly (p<0.05) the least bacterial count. Fungi count varied from 3.65 to 4.62 Log10CFU/g. Conclusion: The minimally processed papaya was low in acidity, rich in ascorbic acid and a good source of the nutrient. Sensory attributes of the products were acceptable to the assessors. Microbial counts were unsatisfactory and can pose a risk factor to public health

Three Dimensional Electrical Impedance Tomography

The electrical resistivity of mammalian tissues varies widely and is correlated with physiological function. Electrical impedance tomography (EIT) can be used to probe such variations in vivo, and offers a non-invasive means of imaging the internal conductivity distribution of the human body. But the computational complexity of EIT has severe practical limitations, and previous work has been restricted to considering image reconstruction as an essentially two-dimensional problem. This simplification can limit significantly the imaging capabilities of EIT, as the electric currents used to determine the conductivity variations will not in general be confined to a two-dimensional plane. A few studies have attempted three-dimensional EIT image reconstruction, but have not yet succeeded in generating images of a quality suitable for clinical applications. Here we report the development of a three-dimensional EIT system with greatly improved imaging capabilities, which combines our 64-electrode data-collection apparatus with customized matrix inversion techniques. Our results demonstrate the practical potential of EIT for clinical applications, such as lung or brain imaging and diagnostic screening

A one-year trial of lamivudine for chronic hepatitis B

Background and Methods: In preliminary trials, lamivudine, an oral nucleoside analogue, has shown promise for the treatment of chronic hepatitis B. We conducted a one-year double-blind trial of lamivudine in 358 Chinese patients with chronic hepatitis B. The patients were randomly assigned to receive 25 mg of lamivudine (142 patients), 100 mg of lamivudine (143), or placebo (73) orally once daily. The patients underwent liver biopsies before entering the study and after completing the assigned treatment regimen. The primary end point was a reduction of at least two points in the Knodell necroinflammatory score. Results: Hepatic necroinflammatory activity improved by two points or more in 56 percent of the patients receiving 100 mg of lamivudine, 49 percent of those receiving 25 mg of lamivudine, and 25 percent of those receiving placebo (P<0.001 and P=0.001, respectively, for the comparisons of lamivudine treatment with placebo). Necroinflammatory activity worsened in 7 percent of the patients receiving 100 mg of lamivudine, 8 percent of those receiving 25 mg, and 26 percent of those receiving placebo. The 100mg dose of lamivudine was associated with a reduced progression of fibrosis (P=0.01 for the comparison with placebo) and with the highest rate of hepatitis B e antigen (HBeAg) seroconversion (loss of HBeAg, development of antibody to HBeAg, and undetectable HBV DNA) (16 percent), the greatest suppression of HBV DNA (98 percent reduction at week 52 as compared with the base-line value), and the highest rate of sustained normalization of alanine aminotransferase levels (72 percent). Ninety-six percent of the patients completed the study. The incidence of adverse events was similar in all groups, and there were few serious events. Conclusions: In a one-year study, lamivudine was associated with substantial histologic improvement in many patients with chronic hepatitis B. A daily dose of 100 mg was more effective than a daily dose of 25 mg.published_or_final_versio

Exhaustive prediction of disease susceptibility to coding base changes in the human genome

<p>Abstract</p> <p>Background</p> <p>Single Nucleotide Polymorphisms (SNPs) are the most abundant form of genomic variation and can cause phenotypic differences between individuals, including diseases. Bases are subject to various levels of selection pressure, reflected in their inter-species conservation.</p> <p>Results</p> <p>We propose a method that is not dependant on transcription information to score each coding base in the human genome reflecting the disease probability associated with its mutation. Twelve factors likely to be associated with disease alleles were chosen as the input for a support vector machine prediction algorithm. The analysis yielded 83% sensitivity and 84% specificity in segregating disease like alleles as found in the Human Gene Mutation Database from non-disease like alleles as found in the Database of Single Nucleotide Polymorphisms. This algorithm was subsequently applied to each base within all known human genes, exhaustively confirming that interspecies conservation is the strongest factor for disease association. For each gene, the length normalized average disease potential score was calculated. Out of the 30 genes with the highest scores, 21 are directly associated with a disease. In contrast, out of the 30 genes with the lowest scores, only one is associated with a disease as found in published literature. The results strongly suggest that the highest scoring genes are enriched for those that might contribute to disease, if mutated.</p> <p>Conclusion</p> <p>This method provides valuable information to researchers to identify sensitive positions in genes that have a high disease probability, enabling them to optimize experimental designs and interpret data emerging from genetic and epidemiological studies.</p

Superpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression.

Aim: To evaluate the effects of preventive treatment with low-level laser therapy (LLLT) on progression of dystrophy in mdx mice. Methods: Ten animals were randomly divided into 2 experimental groups treated with superpulsed LLLT (904 nm, 15 mW, 700 Hz, 1 J) or placebo-LLLT at one point overlying the tibialis anterior muscle (bilaterally) 5 times per week for 14 weeks (from 6th to 20th week of age). Morphological changes, creatine kinase (CK) activity and mRNA gene expression were assessed in animals at 20th week of age. Results: Animals treated with LLLT showed very few morphological changes in skeletal muscle, with less atrophy and fibrosis than animals treated with placebo-LLLT. CK was significantly lower (p = 0.0203) in animals treated with LLLT (864.70 U.l−1, SEM 226.10) than placebo (1708.00 U.l−1, SEM 184.60). mRNA gene expression of inflammatory markers was significantly decreased by treatment with LLLT (p<0.05): TNF-α (placebo-control = 0.51 µg/µl [SEM 0.12], - LLLT = 0.048 µg/µl [SEM 0.01]), IL-1β (placebo-control = 2.292 µg/µl [SEM 0.74], - LLLT = 0.12 µg/µl [SEM 0.03]), IL-6 (placebo-control = 3.946 µg/µl [SEM 0.98], - LLLT = 0.854 µg/µl [SEM 0.33]), IL-10 (placebo-control = 1.116 µg/µl [SEM 0.22], - LLLT = 0.352 µg/µl [SEM 0.15]), and COX-2 (placebo-control = 4.984 µg/µl [SEM 1.18], LLLT = 1.470 µg/µl [SEM 0.73]). Conclusion: Irradiation of superpulsed LLLT on successive days five times per week for 14 weeks decreased morphological changes, skeletal muscle damage and inflammation in mdx mice. This indicates that LLLT has potential to decrease progression of Duchenne muscular dystrophy

Subtidal macrozoobenthos communities from northern Chile during and post El Niño 1997–1998

Despite a large amount of climatic and oceanographic information dealing with the recurring climate phenomenon El Niño (EN) and its well known impact on diversity of marine benthic communities, most published data are rather descriptive and consequently our understanding of the underlying mechanisms and processes that drive community structure during EN are still very scarce. In this study, we address two questions on the effects of EN on macrozoobenthic communities: (1) how does EN affect species diversity of the communities in northern Chile? and (2) is EN a phenomenon that restarts community assembling processes by affecting species interactions in northern Chile? To answer these questions, we compared species diversity and co-occurrence patterns of soft-bottoms macrozoobenthos communities from the continental shelf off northern Chile during (March 1998) and after (September 1998) the strong EN event 1997–1998. The methods used varied from species diversity and species co-occurrence analyses to multivariate ordination methods. Our results indicate that EN positively affects diversity of macrozoobenthos communities in the study area, increasing the species richness and diversity and decreasing the species dominance. EN represents a strong disturbance that affects species interactions that rule the species assembling processes in shallow-water, sea-bottom environments

Computational Cancer Biology: An Evolutionary Perspective

ISSN:1553-734XISSN:1553-735

Dental management considerations for the patient with an acquired coagulopathy. Part 1: Coagulopathies from systemic disease

Current teaching suggests that many patients are at risk for prolonged bleeding during and following invasive dental procedures, due to an acquired coagulopathy from systemic disease and/or from medications. However, treatment standards for these patients often are the result of long-standing dogma with little or no scientific basis. The medical history is critical for the identification of patients potentially at risk for prolonged bleeding from dental treatment. Some time-honoured laboratory tests have little or no use in community dental practice. Loss of functioning hepatic, renal, or bone marrow tissue predisposes to acquired coagulopathies through different mechanisms, but the relationship to oral haemostasis is poorly understood. Given the lack of established, science-based standards, proper dental management requires an understanding of certain principles of pathophysiology for these medical conditions and a few standard laboratory tests. Making changes in anticoagulant drug regimens are often unwarranted and/or expensive, and can put patients at far greater risk for morbidity and mortality than the unlikely outcome of postoperative bleeding. It should be recognised that prolonged bleeding is a rare event following invasive dental procedures, and therefore the vast majority of patients with suspected acquired coagulopathies are best managed in the community practice setting

Challenges for Allergy Diagnosis in Regions with Complex Pollen Exposures

Over the past few decades, significant scientific progress has influenced clinical allergy practice. The biological standardization of extracts was followed by the massive identification and characterization of new allergens and their progressive use as diagnostic tools including allergen micro arrays that facilitate the simultaneous testing of more than 100 allergen components. Specific diagnosis is the basis of allergy practice and is always aiming to select the best therapeutic or avoidance intervention. As a consequence, redundant or irrelevant information might be adding unnecessary cost and complexity to daily clinical practice. A rational use of the different diagnostic alternatives would allow a significant improvement in the diagnosis and treatment of allergic patients, especially for those residing in complex pollen exposure areas