Research on Multi-specialty Coordination, Multi-discipline and Multifunction Integration Oriented Modeling and Simulation Innovation Technology

AbstractThe paper mainly covers the connotations, the functions and the key techniques of the Aircraft Digital Cooperative Robust Integration Optimization Performance Simulation Technology. The Performance Simulation Airplane Integration Design Platform, which has been established by the Performance Simulation Airplane construction analysis, a series of Standard Specification Establishing, the key modules development and a series of the supporting work implementation. It can be customized and be extended, and it is easy to be demonstrated. The platform contains the engineering database including the Performance Simulation Airplane model, simulation process data, simulation results data, and so on. It also provides the closed development process from the initial design to the optimized design, and end to the final detail design. It carries out the configuration selection and performance analysis for the airplane various configurations under the product development requirements in the entire process. It is applicable for the cooperative simulation analysis about the General Configuration research, the Aerodynamic design, the Structure design, the Strength design and some system specialties. This technique system has brought revolutionary changes to the traditional technological concept and Architecture, and it has been proved that the design period of the product is shortened by more than 25%, and the simulation test period is also shortened by more than 25%

Antioxidant Promotion of Tyrosine Nitration in the Presence of Copper(II)

Copper(II) is known to catalyze the generation of reactive nitrogen species in the presence of hydrogen peroxide, nitrite or nitric oxide, leading to tyrosine nitration, a biomarker for free radical species associated diseases. Here, we find that biological antioxidants such as ascorbic acid can promote tyrosine nitration in the presence of copper(II) and nitrite under aerobic and weak acidic conditions. Tyrosine nitration is demonstrated on both β-amyloid peptide and angiotensin I. These studies show that i) ascorbic acid works as pro-oxidant in the presence of copper(II) to induce oxidation and nitration on peptides, ii) both free and coordinated copper(II) can catalyze peptide oxidation and nitration, iii) nitration occurs under mildly acidic conditions (pH = 6.0 - 6.5)

Insight into the effect of hospital-based prehabilitation on postoperative outcomes in patients with total knee arthroplasty: A retrospective comparative study

Background: Osteoarthritis (OA) has become one of the most prevalent joint diseases worldwide, leading to a growing burden of pain and disability as populations age. Although there is consistent evidence to support postoperative rehabilitation and high-intensity prehabilitation for total knee arthroplasty (TKA), the clinical outcomes of hospital-based prehabilitation remain unclear. We aimed to evaluate the effect of a hospital-based prehabilitation program on knee score (KS), function score (FS), and length of stay (LOS) among patients with knee OA after TKA. Methods: A retrospective comparative study was conducted at Renmin Hospital of Wuhan University among patients with primary knee OA. Seventy-two postopearative patients who did not undergo the prehabilitation program were included as the control group, while 68 postoperative patients who underwent the prehabilitation program were assigned to the intervention group. All patients went through the same care after TKA. The KS, FS, and pain levels were measured 5 days before surgery, immediately preceding surgery, immediately after the surgery, and at 1 week and 1 month postoperatively. LOS for each patient was recorded. Results: The new prehabilitation training program significantly improved the KS over time in the intervention group. However, no significant between-group difference was identified in the change of FS. The prehabilitation program also provided shorter LOS. Conclusions: The hospital-based prehabilitation program leads to improved recovery, as indicated by higher KS postoperatively, which may result in improved clinical outcomes of TKA

Effect of Fluorescent Particle Size on the Modulation Efficiency of Ultrasound-Modulated Fluorescence

To investigate whether the size of fluorescent particles affects the modulation efficiency of ultrasound-modulated fluorescence (UMF), we measured UMF and DC (direct current) signals of the fluorescence emission from four different-sized fluorescent particles: (1) three carboxylate-modified fluorescent microspheres (FM) with diameters of 20 nm, 200 nm, and 1.0 μm and (2) streptavidin-conjugated Alexa Fluor 647 with a diameter of approximately 5 nm. The UMF and DC signals were simultaneously measured using a broadband lock-in amplifier and a narrowband amplifier, respectively. The ratio of the UMF strength to the DC signal strength is defined as the modulation efficiency. This modulation efficiency was then used to evaluate the effects of fluorophore size and concentration. Results show that the modulation efficiency was improved by approximately a factor of two when the size of the fluorescent particles is increased from 5 nm to 1 μm. In addition, the linear relationship between the UMF strength and ultrasound pressure (observed in our previous study) was maintained regardless of the fluorescent particle sizes

Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum

A bacterial artificial chromosome (BAC) library for G. mustelinum Miers ex G. Watt (AD4) was constructed. Intact nuclei from G. mustelinum (AD4) were used to isolate high molecular weight DNA, which was partially cleaved with Hind III and cloned into pSMART BAC (Hind III) vectors. The BAC library consisted of 208,182 clones arrayed in 542 384-microtiter plates, with an average insert size of 121.72 kb ranging from 100 to 150 kb. About 2% of the clones did not contain inserts. Based on an estimated genome size of 2372 Mb for G. mustelinum, the BAC library was estimated to have a total coverage of 10.50 × genome equivalents. The high capacity library of G. mustelinum will serve as a giant gene resource for map-based cloning of quantitative trait loci or genes associated with important agronomic traits or resistance to Verticillium wilt, physical mapping and comparative genome analysis

Nanomaterial-assisted laser desorption ionization for mass spectrometry-based biomedical analysis

Nanomaterials have been widely used to assist laser desorption ionization of biomolecules for mass spectrometry analysis. Compared with classical matrix-assisted laser desorption ionization, strategies based on nanomaterial-assisted ionization generate a clean background, which is of great benefit for the qualitative and quantitative analysis of small biomolecules, such as therapeutic and diagnostic molecules. As label-free platforms, they have successfully been used for high-throughput enzyme activity/inhibition monitoring and also for tissue imaging to map in situ the distribution of peptides, metabolites and drugs. In addition to widely used porous silicon nanomaterials, gold nanoparticles can be easily chemically modified by thiol-containing compounds, opening novel interesting perspectives. Such functionalized nanoparticles have been used both as probes to extract target molecules and as matrices to assist laser desorption ionization for developing new enzyme immunoassays or for studying DNA hybridization. More recently, semiconductor nanomaterials or quantum dots acting as photosensitive centers to induce insource redox reactions for proteomics and to investigate biomolecule oxidation for metabolomics have been shown to offer new analytical strategies