Essential Oil Composition of Two Grammosciadium DC Species, G. platycarpum (Boiss et Hausskn) Schischk and G. macrodon Boiss (Apiaceae), from Turkey

Purpose: To investigate and compare the essential oil composition of two Grammosciadium species obtained by hydrodistillationMethods: The essential oil of the aerial parts of two species was obtained by hydrodistillation and analyzed by gas chromatography (GC) and gas chromatography - mass spectrometry (GC-MS).Results: In G. platycarpum, twenty-seven compounds were identified representing 99.3 % of the total oil. The major constituents of the oil were limonene (28.4 %), β-pinene (16.1 %), β-selinene (15.9 %), δ- 3-carene (11.2 %), α-farnesene (6.0 %) and p - cymene (4.7 %). The oil of G. macrodon contained thirty-seven components representing 94.4 % of the total oil, with caryophyllene oxide (15.9 %), germacrene - D (12.4 %), β-caryophyllene (11.5 %), δ-3 - carene (10.1 %) and cyclohexane (4.6 %) as the main constituents.Conclusion: The essential oil composition of G. macrodon was analysed for the first time, the most abundant constituents being caryophyllene oxide, and cyclohexane.Keywords: Grammosciadium macrodon, Grammosciadium platycarpum, Essential oil, Limonene, Caryophyllene oxide, Germacrene - D, β-Caryophyllene, δ-3-Caren

Ornithogalum nurdaniae (Liliaceae), a New Species from North-West Anatolia, Turkey

WOS: 000268124100002Ornithogalum nurdaniae Y. Bagcl & Savran sp. nov. (Liliaceae), a new species related to O. oligophyllum E.D.Clarke. is described and illustrated from Uludag (the Bithynian Olympus), a mountain in NW Anatolia well known for its rich flora and many local endemics (A2 Bursa province). Diagnostic morphological characters are discussed. Notes are also presented on the ecology, biogeography. and conservation status of the new species.TUBITAK [106T179]This new species was found during our study entitled "Revision of the genus Barbarea R.Br. (Cruciferae) in Turkey". We are grateful for TUBITAK (Project number: 106T179) for its financial support of this study. The authors thank Professor Dr. inasi Yildirimli for his help with the Latin diagnosis

Chemical composition of essential oil of Hyssopusofficinalis L. And Origanumacutidens

International audienceThe chemical constituents of essential oil by hydrodistillation from the aerial parts of Hyssopus officinalis L. and Origanum acutidens (hand.-mazz.) ietswaart from Turkey were identified by gas chromatograph-mass spectrometer (GC-MS) demonstrated the presence of at least 24 compounds. The major constituents of H. officinalis oil were pinocarvone (29.2 %), trans-pine camphone (27.2 %), β-pinene (17.6 %), cis-pinocamphone (4.7 %) and myrcene (2.92 %). The major constituents of the essential oil of O. acutidens were carvacrol (76.2 %), p-cymene (7.4 %) and borneol (3.2 %)

A Fast and Parallel Stroud-Based Stochastic Collocation Method for Statistical EMI/EMC Analysis

A fast and parallel Stroud-based stochastic collocation method for statistically characterizing electromagnetic interference and compatibility (EMI/EMC) phenomena on loaded multiscale platforms with uncertain system configurations and subject to variable electromagnetic excitations is described. The proposed method uses a previously developed hybrid time domain integral equation based field-cable-circuit to carry out deterministic EMI/EMC simulations permitting the statistical characterization of pertinent observables. The number of simulations required by the proposed method is far fewer than those needed by Monte-Carlo methods. The proposed method is used to characterize cable-induced coupling onto PC cards located in shielding enclosures. Both the hybrid simulator and the stochastic collocation code execute with near-full efficiency on distributed memory clusters

Assessment of the MRI and Behavioral Test Results in a Focal Cerebral Ischemia-Reperfusion Model in the Rat after Separate and Combined Use of Mouse-Derived Neural Progenitor Cells, Human-Derived Neural Progenitor Cells and Atorvastatin

AIM: To assess the efficacy of Neural progenitor cell (NPC) transplantation in ischemic stroke, and to investigate whether atorvastatin enhances therapeutic potency of NPC after stroke. MATERIAL and METHODS: The focal cerebral ischemia-reperfusion model was performed by transient occlusion of middle cerebral artery. Rats were assigned randomly to receive intracerebral transplantation of mouse NPC alone (mNPC), human NPC alone (hNPC), mouse NPC plus oral atorvastatin (mNPC+A), human NPC plus oral atorvastatin (hNPC+A), oral atorvastatin alone, or intracerebral Dulbecco’s Modified Eagle’s medium injection (control group). Adhesive removal, rotarod, cylinder tests, and magnetic resonance imaging (MRI) were used for assessment of rats during 4 weeks. After sacrification on 28th day, rats were investigated by immunofluorescent staining. RESULTS: The hNPC and mNPC groups showed significantly improved functional outcome and reduced infarct area ratio compared with the control group. The hNPC group had significantly better performance and lower infarct area ratio than the mNPC group. Addition of atorvastatin to stem cell therapy significantly improved functional outcome, although it did not affect the infarct area ratio on MRI. Anti-inflammatory response in the infarct area was higher in the mNPC group. NPC transplantation significantly reduced the amount of microglia and a significant increase in the amount of astrocytes. CD8a+ T lymphocyte and granzyme B activities were not detected in any of the subjects. CONCLUSION: Both hNPC and mNPC treatments significantly improved functional outcome, and reduced infarct area ratio after stroke. Atorvastatin enhanced the therapeutic potency of NPCs, including neurological improvement

Investigation of microRNA expression changes in HepG2 cell line in presence of URG4/URGCP and in absence of URG4/URGCP suppressed by RNA interference

Hepatocellular carcinoma (HCC) originates from liver cells and is one of the most common malignant cancers in the world. microRNAs (miRNA), are single strand non-coding RNA molecules with the length of 18-25 nucleotides. miRNAs play an important role in the development of HCC, i.e., miRNAs have a significant impact on multistep hepatocellular carcinogenesis including cellular migration and invasion. URG4/URGCP (up-regulated gene-4/upregulator of cell proliferation) is up-regulated in the presence of HBxAg and has been identified and characterized by Satiroglu-Tufan et al. The full-length URG4/URGCP is 3.607 kb. Overexpression of URG4/URGCP in the presence of HBV X protein may function as a putative oncogene that significantly contributes to multi-step hepatocarcinogenesis. In this study, we aimed to investigate potential miRNA expression changes in HepG2 cell line model system in the presence of URG4/URGCP and in the absence of URG4/URGCP, which was suppressed by RNA interference. To functionally characterize URG4/URGCP, independent cultures of HepG2 cells were stably transfected with pcDNA3 or pcDNA3-URG4/URGCP. Relative quantification of whole genome miRNAs was analyzed by RT-PCR using human whole genome miRNA qPCR profiling kits. Among the 1,034 human miRNAs investigated by the arrays, 77 miRNAs were up-regulated and nine miRNAs were down-regulated in the presence of URG4/URGCP. In conclusion, we have analyzed miRNA profiles in HepG2 cells in presence or absence of URG4/URGCP gene using RNA interference. Some of these miRNAs may play roles in URG4/URGCP gene related disease development through the regulation of different signaling pathways

Erratum to: Investigation of microRNA expression changes in HepG2 cell line in presence of URG4/URGCP and in absence of URG4/URGCP suppressed by RNA interference (vol 39, pg 11119, 2012)

WOS: 00032594840005

Public Health Rep

19315293PMCnul

Chemical Composition of The Essential Oil of The Flowers and Leaves of Calba Tea (Dorystoechas hastata Boiss & Helder. ex Bentham)

WOS: 000381628100034The essential oils of flower and leaves of Dorystoechas hastata Boiss & Helder. ex Bentham, grown in Turkey, was obtained by hydrodistillation and analysed by GC and GS-MS. Fourty components were identified in the flowers oil, which represented about 93.97 % of the total composition of the oil. The major constituents of the flower essential oil were myrcene (19.37 %), 1,8-cineole (14.30 %), beta-pinene (9.19 %), alpha-pinene (8.49 %) and beta-caryophyllene (6.18 %). Thirty-seven components were established in the leaves oil, making up 97.16 % of the total composition. The main constituents of the essential oil of the leaves were myrcene (20.71 %), 1,8-cineole (18.76 %), beta-pinene (12.51 %), alpha-pinene (8.54 %), bornyl acetate (7.28 %) and terpinene-4-ol (6.19 %). As seen, myrcere, 1,8-cineole and beta-pinene for both oils were the main components.Selcuk University Coordination of Scientific Research Projects (S.U.-BAP, Konya-Turkey)This study was supported by Selcuk University Coordination of Scientific Research Projects (S.U.-BAP, Konya-Turkey)