The Potential of the Science-Religion Confluence for Affecting Policy and Administration

The author's understanding of the full scope of God's covenant after the Flood, together with the scientific insights of systems theory, guided him to implement a new approach in administering national restoration and protection policy. He is convinced that attempts by Congress to dismantle such legislation as the Endangered Species Act of 1973 are founded upon fear of the potential power of the conjunction of spiritual values with scientific insight

Biases in the Experimental Annotations of Protein Function and their Effect on Our Understanding of Protein Function Space

The ongoing functional annotation of proteins relies upon the work of curators to capture experimental findings from scientific literature and apply them to protein sequence and structure data. However, with the increasing use of high-throughput experimental assays, a small number of experimental studies dominate the functional protein annotations collected in databases. Here we investigate just how prevalent is the "few articles -- many proteins" phenomenon. We examine the experimentally validated annotation of proteins provided by several groups in the GO Consortium, and show that the distribution of proteins per published study is exponential, with 0.14% of articles providing the source of annotations for 25% of the proteins in the UniProt-GOA compilation. Since each of the dominant articles describes the use of an assay that can find only one function or a small group of functions, this leads to substantial biases in what we know about the function of many proteins. Mass-spectrometry, microscopy and RNAi experiments dominate high throughput experiments. Consequently, the functional information derived from these experiments is mostly of the subcellular location of proteins, and of the participation of proteins in embryonic developmental pathways. For some organisms, the information provided by different studies overlap by a large amount. We also show that the information provided by high throughput experiments is less specific than those provided by low throughput experiments. Given the experimental techniques available, certain biases in protein function annotation due to high-throughput experiments are unavoidable. Knowing that these biases exist and understanding their characteristics and extent is important for database curators, developers of function annotation programs, and anyone who uses protein function annotation data to plan experiments.Comment: Accepted to PLoS Computational Biology. Press embargo applies. v4: text corrected for style and supplementary material inserte

Amphibian Contributions to Ecosystem Services

Ecosystems provide essential services for human society, which include provisioning, regulating, cultural, and supporting services. Amphibians provide provisioning services by serving as a food source for some human societies, especially in Southeast Asia. They also serve as models in medical research and provide potential for new pharmaceuticals such as analgesics and anti-viral drugs derived from skin secretions. Amphibians contribute to regulating services by reducing mosquito recruitment from ephemeral wetlands, potentially controlling other pest species, and indirectly through predation of insect pollinators. Often neglected, ecosystems also provide cultural services to human societies that increase the quality of human life through recreation, religion, spirituality, and aesthetics. As an abundant and diverse class of vertebrates, amphibians also play prominent roles in the culture of human societies through pathways such as mythology, literature, and art. Most research on the role of amphibians in ecosystems has been on their contribution to supporting services. This is also the area where amphibians are likely to have the largest contribution to ecosystem services. Supporting services have structural (e.g., habitat) and functional (e.g., ecosystem functions and processes) components. Amphibians can affect ecosystem structure through soil burrowing and aquatic bioturbation and ecosystem functions such as decomposition and nutrient cycling through waste excretion and indirectly through predatory changes in the food web. They also can control primary production in aquatic ecosystems through direct consumption and nutrient cycling. Unfortunately, amphibians are experiencing major declines and humans may be losing associated ecosystem services. It is important to understand how declines affect ecosystem services for human societies, but these declines can also serve as natural experiments to understand the role of amphibians in ecosystems

An Experimental Test of Buffer Utility as a Technique for Managing Pool-Breeding Amphibians

Vegetated buffers are used extensively to manage wetland-dependent wildlife. Despite widespread application, buffer utility has not been experimentally validated for most species. To address this gap, we conducted a six-year, landscape-scale experiment, testing how buffers of different widths affect the demographic structure of two amphibian species at 11 ephemeral pools in a working forest of the northeastern U.S. We randomly assigned each pool to one of three treatments (i.e., reference, 100m buffer, 30m buffer) and clearcut to create buffers. We captured all spotted salamanders and wood frogs breeding in each pool and examined the impacts of treatment and hydroperiod on breeding-population abundance, sex ratio, and recapture rate. The negative effects of clearcutting tended to increase as forest-buffer width decreased and be strongest for salamanders and when other stressors were present (e.g., at short-hydroperiod pools). Recapture rates were reduced in the 30m, but not 100m, treatment. Throughout the experiment for frogs, and during the first year post-cut for salamanders, the predicted mean proportion of recaptured adults in the 30m treatment was only 62% and 40%, respectively, of that in the reference treatment. Frog sex ratio and abundance did not differ across treatments, but salamander sex ratios were increasingly male-biased in both cut treatments. By the final year, there were on average, only about 40% and 65% as many females predicted in the 100m and 30m treatments, respectively, compared to the first year. Breeding salamanders at short-hydroperiod pools were about 10% as abundant in the 100m versus reference treatment. Our study demonstrates that buffers partially mitigate the impacts of habitat disturbance on wetland-dependent amphibians, but buffer width and hydroperiod critically mediate that process. We provide the first experimental evidence showing that 30-m-wide buffers may be insufficient for maintaining resilient breeding populations of pool-dependent amphibians, at least during the first six years post-disturbance

Adherence to Exercise Prescription and Improvements in the Clinical and Vascular Health of African Americans

International Journal of Exercise Science 10(2): 246-257, 2017. Improvements in indices of vascular health and endothelial function have been inversely associated with hypertension, a risk factor for cardiovascular disease (e.g., myocardial infarction, stroke, and heart failure), renal failure, and mortality. Aerobic exercise training (AEXT) has been positively associated with improvements in clinical health values, as well as vascular health biomarkers, and endothelial function. The purpose of this study was to evaluate whether measures of exercise adherence were related to clinical outcome measures and indices of vascular health subsequent to a 6-month AEXT intervention in a middle-to-older aged African American cohort. Following dietary stabilization, sedentary, apparently healthy, African American adults (40 - 71 y/o) underwent baseline testing including blood pressure, flow-mediated dilation (FMD) studies, fasting blood sampling, and graded exercise testing. Upon completion of a supervised 6-month AEXT intervention, participants repeated all baseline tests. Exercise adherence was measured three ways: exercise percentage, exercise volume, and exercise score. There were no significant correlations between the changes in the vascular health biomarkers of the participants and any of the adherence measures. In addition, there were no significant correlations between any of the adherence measures and the clinical values of the participants that had been significantly changed pre-post-AEXT. Participants improved their clinical and vascular health and decreased risk factors for hypertension and cardiovascular disease regardless of their level of adherence to AEXT. Future studies should continue to accurately quantify adherence in order to assess the exercise dose for improvements in vascular and clinical health