Inseminação artificial em caprinos leiteiros com sêmen resfriado por 24 ou 48 horas.

Resumo - Objetivou-se, avaliar a viabilidade da utilização do sêmen caprino diluído em meio tris-gema 2,5% resfriado a 5°C e armazenado por diferentes períodos (24 ou 48 horas). Foram inseminadas por via transcervical 133 cabras sem raça definida e nativas da República de Cabo Verde, divididas aleatoriamente em dois tratamentos T24 e T48. O estro foi sincronizado com a utilização de esponjas intra-vaginais contendo 60 mg de acetato de medroxiprogesterona por seis dias; 37,5 ?g de D-cloprostenol e 200 UI de eCG 24 horas antes da retirada da esponja. Foram utilizados três reprodutores da Raça Canárias. Para resfriar e manter o sêmen a 5o C foi utilizado o Botutainer® (Biotech Botucatu, Reprodução Animal, Botucatu - SP) adaptado. Não houve diferença (P > 0,05) entre os padrões seminais para os diferentes períodos de resfriamento (T24 - 58,8% ± 11,1 de motilidade e 2,9 ± 0,5 de vigor; T48 - 51,3% ± 2,5 de motilidade e 2,8 ± 0,3 de vigor), o que permitiu obter taxas de parição similares em ambos os tratamentos (T24 - 26,5% e T48 - 21,5%). A eficiência dos protocolos testados permitiu a disseminação de genética caprina na República de Cabo Verde. Conclui-se que o sêmen caprino, resfriado por 48 horas a 5ºC, tem o mesmo potencial de fertilização do sêmen resfriado por 24 horas a 5ºC. Artificial insemination in dairy goats with cooled semen stored for 24 or 48 hours. Abstract - The fertilizing capacity of goat semen diluted in tris-egg yolk 2.5% chilled at 5oC for 24 or 48 hours was evaluated. Transcervical artificial insemination was performed in 133 goats that were divided, randomly, into two treatments T24 and T48. The estrus was synchronized by the mean of intra-vaginal sponges containing 60 mg of medroxyprogesterone acetate for six days; 37.5 mg of D-cloprostenol and 200 IU of eCG, 24 hours before removing the sponge. Three Canarian?s Buck were used. For cooling and keeping the semen at 5oC a Botutainer® (Biotech Botucatu, Animal Reproduction, Botucatu - SP) was adapted and used. There was no difference (P > 0.05) between the seminal patterns for the different periods of cooling (T24 - 58.8%±11.1 for motility and 2.9±0.5 for strength; T48 - 51.3%±2.5 for motility and 2.8±0.3 for strength), which allowed to obtain similar pregnancy rates in both treatments (T24 ? 26.5% and T48 - 21,5%). The efficiency of the tested protocols allowed the dissemination of goat?s genetic material in the Republic of Cape Verde. It was concluded that goat semen, cooled for 48 hours at 5ºC, has the same fertility that semen cooled for 24 hours at 5ºC.Anais do 15o. Simpósio Paranaense de Ovinocultura, 3o. Simpósio Paranaense de Caprinocultura, 3o. Simpósio Sul Brasileiro de Ovinos e Caprinos, Pato Branco, PR, out., 2011

Possíveis impactos do projeto: "Uso de sêmen resfriado e inseminação artificial em caprinos leiteiros na República de Cabo Verde".

Resumo - Este artigo procura discutir o contexto do projeto ?Uso de sêmen resfriado e inseminação artificial em caprinos leiteiros na República de Cabo Verde?, suas implicações no sistema de criação de caprinos leiteiros, bem como seu impacto nos planos social, ambiental e econômico. Possible impacts of the project: "use of chilled semen and artificial insemination in dairy goats on Cape Verde Republic?. Abstract - This article intent to discuss the aspects involved in the project ?Use of chilled semen and artificial insemination in dairy goats on Cape Verde Republic? their implications on dairy goats rearing and also, the social, the economic, and the environmental impacts.Anais do 15o. Simpósio Paranaense de Ovinocultura, 3o. Simpósio Paranaense de Caprinocultura, 3o. Simpósio Sul Brasileiro de Ovinos e Caprinos, Pato Branco, PR, out., 2011

Estabelecimento de protocolos para seleção e indução da capacitação espermática em caprinos.

[Establishment of protocols for selection and induction of sperm capacitation in goat]

Avaliação da formação de corpos lúteos acessórios e dosagem de progesterona em cabras da raça Saanen após o uso de hCG.

Avalia a formação de CLa e concentração plasmática de progesterona em decorrência da administração de hCG em cabras cinco dias após a última monta natural. Conclui-se que o tratamento com hCG 5 dias após a última MN induziu um efeito luteotrópico, e não a formação de CLa, antecipando o momento da ocorrência da elevação das concentração de progesterona plasmática. [Evaluation of formation of accessories corpora lutea and plasma progesterone concentration in Saanen goats treated with hCG]

Avaliação do uso da prostaglandina na sincronização do estro associado ou não ao uso do hCG em cabras.

Resumo: O objetivo deste estudo foi avaliar a eficácia da sincronização do estro sobre os parâmetros ovarianos e comportamentais de cabras da raça Toggenburg. A administração da hCG no inicio do estro é capaz de antecipar a ovulação e promover um aumento no numero de ovulações. Estes resultados devem ser observados para estabelecimento momento ideal para a IA. [Evaluation of the use prostaglandin in synchronization the estrus associated or not the hCG in goats]

Reproductive parameters of dairy goats submitted to estrus synchronization with prostaglandin F2alfa associated or not to hCG at estrous onset.

Abstract: The objective of this study was to evaluate the efficiency of two doses of PGF associated or not to hCG on the associated reproductive parameters in dairy goats. A total of 29 goats received two doses of 30 ug d-cloprostenol latero-vulvar at a 10 day intervals (Day 1 and Day 10). The does were allocated according to body weight and body condition score into two treatments, to receive hCG (250IU) or saline at estrus onset. After the second dose of PGF, estrus was monitored and ultrasound exams were performed twice daily. All does were inseminated 16h after estrus onset. Blood collection was performed every day for progesterone assay. The use of hCG at estrus onset did not affect any studied parameter and therefore the data were pooled. Estrous response rate was similar (P>0.05) after the first (75.9%, 22/29) and the second dose of PGF (79.3%, 23/29). The interval between the administration of PGF and estrus onset was greater (P0.05) between both experimental groups were detected in the following parameters, averaging: the interval from the second dose administration to the ovulation (86.6±11.4h), interval from estrus to ovulation (39.9±12.3 h), diameter of largest follicle (7.2±1.4) and number of ovulations (1.8±0.6). At Day 1, 52.4% (11/21) of does presented progesterone concentrations 1ng/mL. The results of the present study indicate that estrus can be efficiently synchronized in dairy goats with the use of two doses of PGF at a 10 day interval. Further research should be done evaluating hCG use in different doses or moments of administration. [Parâmetros ovarianos de cabras leiteira submetidas a sincronização do estro com protaglandina F2 alfa associada ou não ao hCG no início do estro]. Resumo: O objetivo deste estudo foi avaliar a eficiência do uso de duas doses de PGF associadas ou não à administração de hCG no início do estro sobre os parâmetros reprodutivos de cabras leiteiras. Um total de 29 cabras receberam duas doses de 30 ?g d-cloprostenol pela via latero-vulvar com 10 dias de intervalo (Dia 1 e Dia 10). As cabras foram alocadas para receberem o hCG (250 IU) ou salina i.m. no momento em que o estro foi detectado. Depois da realização da segunda dose de PGF, o estro foi monitorado e exames ultrassonográficos foram realizados duas vezes ao dia. Todas as fêmeas foram inseminadas 16 h após o inicio do estro. Amostras de sangue foram coletadas diariamente para determinação das concentrações plasmáticas de progesterona. O uso do hCG no momento do início do estro não afetou os parâmetros estudados e, portanto, os dados serão apresentados agrupados. A taxa de manifestação de estro foi similar (P > 0,05) na primeira (75,9% - 22/29) ou na segunda dose de PGF (79,3% - 23/29). O intervalo entre a administração de PGF e o início do estro foi maior (P 0,05) entre os grupos experimentais quanto aos parâmetros reprodutivos: intervalo entre a aplicação da segunda dose e a ovulação (86,6±11,4h), intervalo do estro a ocorrência da ovulação (39,9±12,3h), diâmetro do maior folículo (7,2±1,4) e número de ovulações (1,8±0,6). No Dia 1, 52,4% (11/21) apresentavam concentrações de progesterona 1ng/mL. O presente estudo permite concluir que o estro pode ser eficientemente sincronizado em cabras leiteiras com duas doses de PGF intervaladas em 10 dias. Novas pesquisas devem se realizadas para avaliar diferentes doses e momentos de utilização do hCG

Duration of estrus and time of ovulation in superovulated Santa Inês ewes.

Abstract: The aim of this study was to determine the intervals between sponge removal and the onset of estrus, sponge removal and the first detected ovulation, and onset of estrus and the first ovulation, and also to determine the duration of estrus, in superovulated Santa Inês ewes subjected to natural mating (NM) and AI. The trial was done in July and February at Cachoeiras de Macacu?Rio de Janeiro (22°27'S, 43°39'W). Fifteen non-pregnant Santa Inês ewes, age 3.4 ± 1.4 years, weighing 47.8 ± 6.3 kg, with 3.3 ± 0.4 body condition score (scale of 1 to 5), were randomly assigned to 2 groups. Both groups were superovulated using the same protocol and were mated in a crossover design by NM and laparoscopic AI. Estrus was synchronized using intravaginal sponges (60 mg of medroxyprogesterone acetate; Progespon®, Schering Plough Animal Health, São Paulo, Brazil) inserted (Day 0) and maintained for 6 days. On Day 5, the ewes received IM injections of 300 IU of eCG (Novormon®, Schering Plough Animal Health) and 0.0375 mg of cloprostenol (Prolise®, Tecnopec, São Paulo, Brazil). Twelve hours after sponge removal, 0.025 mg of gonadorelin acetate (Gestran®, Tecnopec) was administered IM. Superovulation started 60 h after sponge removal and consisted of 5 IU kg -1 of porcine FSH (pFSH; Pluset®, Hertape Calier, Minas Gerais, Brazil) IM in 6 decreasing doses (25, 25, 15, 15, 10, and 10%) at 12-h intervals. At the first pFSH dose, new sponges were inserted. At the fifth pFSH dose, 0.0375 mg of cloprostenol was administered IM and the sponges were removed. After the sponge removal, the NM group was exposed to rams twice per day for mating, until the end of estrus. In the AI group, estrus was detected using a teaser with the penis diverted. The females were permitted to be mounted twice per day until the end of estrus, and were inseminated with frozen-thawed semen 24 and 36 h after the end of superovulation. The follicular development and ovulation time were observed using real-time ultrasonography (8.0 MHz Pie Medical®, Aquila Vet, Tokyo, Japan) at 12-h intervals. For statistical analysis, a Student's t-test was performed (5% significance level) using the BioEstat program. Results are presented as mean ± standard deviation. The time from sponge removal to onset of estrus and the duration of estrus did not differ between NM and AI groups (31.79 ± 5.94 v. 25.25 ± 10.38 h and 29.89 ± 11.54 v. 26.66 ± 8.67 h, respectively). The time from sponge removal to ovulation and from onset of estrus to the first ovulation were shorter (P < 0.001) in the NM group (32.11 ± 12.72 v. 56.48 ± 15.39 h and 8.61 ± 5.99 v. 32.25 ± 18.57 h, respectively). The time from sponge removal to ovulation in July and February was 56.07 ± 7.27 versus 56.83 ± 20.72 in the AI group and 29.54 ± 0.56 versus 33.83 ± 19.02 in the NM group, respectively, suggesting that the season of the year in a tropical region did not influence the ovulation time for each treatment. Possibly, the mechanical stimulation induced by the contact of the penis with the vagina fornix and by the accessory sex glands fluids in mating hastened the ovulation time in the NM group. The service can shorten the time of ovulation

Evaluation of superovulatory response in Santa Inês ewes by ultrasonography and laparoscopy.

The development and use of non-invasive techniques would reduce risks of surgery sequels on the same animal and use of the donor could be optimized. The aim of this study was to evaluate the accuracy of ultrasonography performed prior to embryo collection to estimate superovulation response in sheep. Fifteen pluriparous Santa Inês sheep, 2 to 5 year old, with an average body weight of 46.79 ± 6.00 kg and body condition score of 2.96 ± 0.32 (1 to 5 scale) were allocated into 3 groups (GI, GII, GIII) in a cross-over design. In GI, intravaginal sponges (60 mg of medroxyprogesterone acetate; Progespon®, Schering Plough Animal Health, São Paulo, Brazil) were inserted (Day 0) and maintained for 14 days, and the superovulatory (SOV) protocol started on Day 12. In GII and GIII, intravaginal sponges were inserted (Day 0) and maintained for 6 days. On Day 5, the animals were treated with 300 IU of eCG (Novormon 5000®, Schering Plough Animal Health) and 5 mg of dinoprost (Lutalyse® Pfizer Animal Health, São Paulo, Brazil) i.m. Animals in GIII received 0.025 mg of gonadorelin acetate (Gestran-Plus®, Tecnopec, São Paulo, Brazil) i.m. 12 h after sponge withdrawal. In GII and GIII, the SOV protocol started 48 h after sponge removal and a new sponge was inserted immediately after its removal. The SOV protocol in all groups consisted of 200 mg of pFSH (Folltropin-V®, Tecnopec) administered in 6 decreasing doses given every 12 h (50/50, 30/30, and 20/20 mg). At the time of the fifth dose of pFSH, 5 mg of dinoprost was administered i.m. and the sponges were removed. Animals were bred by a sexually mature ram twice a day until the end of estrus. Prior to embryo collection, an ultrasonographic evaluation (5 MHz, Aloka SSD-500, Tokyo, Japan) was performed to determine the number of CL present on both ovaries. The number of CL was further evaluated by laparoscopy, when it was possible to detect poor responders or even non-ovulating animals. Statistical analysis was performed using all tests at the 95% confidence interval by SAEG program. Results are presented as mean ± SE. The number of CL was not different among all groups. The total number (GI, GII, and GIII) of CL determined by ultrasonography (9.09 ± 5.01) was not different (P > 0.05) from that observed by laparoscopy (8.87 ± 5.25). A significant correlation (r = 0.56, P < 0.0005) between evaluation performed by ultrasonography and laparoscopy was observed. These results suggest that ultrasonography can be used to determine the response to the superovulatory protocol. As embryo collection in sheep is performed mainly by surgical techniques, the implementation of non-invasive techniques such as ultrasonography could avoid unnecessary surgeries on animals that did not respond to the SOV protocol, therefore preventing early culling of embryo donors. Top Print this pagePrint Email this page View Issue Contents Abstract Export Citation Tools Print Bookmark Email this page Early Alert Subscribe to our Early Alerts for the latest journal issue contents.Edição dos Proceedings of the Annual Conference of the International Embryo Transfer Society, Córdoba, Argentina, 9?12 January 2010