Prenatal Famine and Genetic Variation Are Independently and Additively Associated with DNA Methylation at Regulatory Loci within IGF2/H19

Both the early environment and genetic variation may affect DNA methylation, which is one of the major molecular marks of the epigenome. The combined effect of these factors on a well-defined locus has not been studied to date. We evaluated the association of periconceptional exposure to the Dutch Famine of 1944–45, as an example of an early environmental exposure, and single nucleotide polymorphisms covering the genetic variation (tagging SNPs) with DNA methylation at the imprinted IGF2/H19 region, a model for an epigenetically regulated genomic region. DNA methylation was measured at five differentially methylated regions (DMRs) that regulate the imprinted status of the IGF2/H19 region. Small but consistent differences in DNA methylation were observed comparing 60 individuals with periconceptional famine exposure with unexposed same-sex siblings at all IGF2 DMRs (PBH<0.05 after adjustment for multiple testing), but not at the H19 DMR. IGF2 DMR0 methylation was associated with IGF2 SNP rs2239681 (PBH = 0.027) and INS promoter methylation with INS SNPs, including rs689, which tags the INS VNTR, suggesting a mechanism for the reported effect of the VNTR on INS expression (PBH = 3.4×10−3). Prenatal famine and genetic variation showed similar associations with IGF2/H19 methylation and their contributions were additive. They were small in absolute terms (<3%), but on average 0.5 standard deviations relative to the variation in the population. Our analyses suggest that environmental and genetic factors could have independent and additive similarly sized effects on DNA methylation at the same regulatory site

Reliability of the automatic procedures for locating earthquakes in southwestern Alps and northern Apennines (Italy)

International audienceReliable automatic procedure for locating earthquake in quasi-real time is strongly needed for seismic warning system, earthquake preparedness, and producing shaking maps. The reliability of an automatic location algorithm is influenced by several factors such as errors in picking seismic phases, network geometry, and velocity model uncertainties. The main purpose of this work is to investigate the performances of different automatic procedures to choose the most suitable one to be applied for the quasi-real-time earthquake locations in northwestern Italy. The reliability of two automatic-picking algorithms (one based on the Characteristic Function (CF) analysis, CF picker, and the other one based on the Akaike's information criterion (AIC), AIC picker) and two location methods (“Hypoellipse” and “NonLinLoc” codes) is analysed by comparing the automatically determined hypocentral coordinates with reference ones. Reference locations are computed by the “Hypoellipse” code considering manually revised data and tested using quarry blasts. The comparison is made on a dataset composed by 575 seismic events for the period 2000–2007 as recorded by the Regional Seismic network of Northwestern Italy. For P phases, similar results, in terms of both amount of detected picks and magnitude of travel time differences with respect to manual picks, are obtained applying the AIC and the CF picker; on the contrary, for S phases, the AIC picker seems to provide a significant greater number of readings than the CF picker. Furthermore, the “NonLinLoc” software (applied to a 3D velocity model) is proved to be more reliable than the “Hypoellipse” code (applied to layered 1D velocity models), leading to more reliable automatic locations also when outliers (wrong picks) are present

Did the 1999 earthquake swarm on Gakkel Ridge open a volcanic conduit? a detailed teleseismic data analysis

International audienceIn 1999, a seismic swarm of 237 teleseismically recorded events marked a submarine eruption along the Arctic Gakkel Ridge, later on also analyzed by sonar, bathymetric, hydrothermal, and local seismic studies. We relocated the swarm with the global location algorithm HYPOSAT and analyzed the waveforms of the stations closest to the events by cross-correlation. We find event locations scattered around 85°35 N and 85° E at the southern rift wall and inside the rift valley of the Gakkel Ridge. Waveforms of three highly correlating events indicate a volumetric moment tensor component and highly precise referenced double-difference arrival times lead us to believe that they occur at the same geographical position and mark the conduit located further southeast close to a chain of recently imaged volcanic cones. This result is supported by station residual anomalies in the direction of the potential conduit. Seismicity is focused at the crust–mantle boundary at 16–20 km depth, but ascending toward the potential conduit during the beginning of April 1999, indicating an opening of the vent