Direct effect of p,p'- DDT on mice liver

ABSTRACT Contact with the pesticide dichlorodiphenyltrichloroethane (p,p′-DDT) can be the cause of various harmful effects in humans, wildlife, and the environment. This pesticide is known to be persistent, lipophilic, resistant to degradation, and bioaccumulive in the environment and to be slowly released into bloodstream. Growing evidence shows that exposure to DDT is linked to type 2 diabetes mellitus. Individuals exposed to elevated levels of DDT and its metabolite have an increased prevalence of diabetes and insulin resistance. To evaluate these possible relationships, experiments were performed on eight-week-old female mice, divided into three groups (n = 10 per group): Group 1 received a vehicle-control intraperitoneal (i.p.) injection of sesame oil; Groups 2 and 3 received an i.p. dose of 50 and 100 µg/g p,p′-DDT respectively, dissolved in sesame oil. All groups were treated once daily for four days. Real-time PCR analysis of several genes was undertaken. Additionally, biochemical parameters and histopathological changes were measured. NQO1, HMOX1, NR1I3 and NR3C1 were up-regulated in DDT-exposed animals compared to the vehicle control group, while only SREBP1 was down-regulated in the 100 µg/g group. MTTP and FABP5, not previously reported for DDT exposure, but involved in regulation of fatty acid fluxes, could also function as biomarkers cross-talking between these signaling pathways. These results suggest that beyond epidemiological data, there is increasing molecular evidence that DDT may mimic different processes involved in diabetes and insulin resistance pathways

Cardiovascular disease and inflammation

Polycystic ovary syndrome (PCOS) is not only a reproductive disorder, but also a complex, multifaceted, endocrine disease with several associated health complications. In fact, multiple lines suggest an increased cardiovascular risk and cardiovascular disease characterized by an impairment of cardiac structure and function, endothelial dysfunction, lipid abnormalities, and low-grade chronic inflammation. The increased prevalence of low-grade chronic inflammation in women with PCOS represents an emerging novel mechanism for cardiovascular disease in these women. All these features are likely linked to the insulin-resistance often present in women with PCOS. Cardiovascular disease and inflammation represent important long-term sequelae of PCOS that warrant further in-depth investigation