EIN2 and COI1 control the antagonism between ethylene and jasmonate in adventitious rooting of Arabidopsis thaliana thin cell layers

Auxins induce adventitious roots (ARs) in numerous culture-systems, and indole-3-butyric acid (IBA) is frequently the best AR-inducer. Vitamin requirements vary according to species, explant, and culture-conditions. Arabidopsis thaliana thin cell layers (AtTCLs) are uncapable of AR-formation on hormone-free medium containing thiamine and myo-inositol, whereas ARs are induced when IBA (10 μM), with/without kinetin (Kin, 0.1 μM), is added. The research frst aim was to determine whether a synergism between IBA and myo-inositol and thiamine was necessary for AR-formation. Results showed that IBA induced AR-formation without myo-inositol and thiamine, but better when both vitamins were also present. Deciphering hormonal action on AR formation under optimal vitamin content would be essential for improving the AR process. Ethylene (ET)/jasmonic acid (JA) signaling cross-talk has been demonstrated as being involved in AR-formation in IBA+Kincultured AtTCLs, by using ein3eil1 and coi1-16 mutants. ETHYLENE INSENSITIVE3 (EIN3)/EIN3-LIKE1 (EIL1) are positive regulators of ethylene (ET)-signaling, whereas CORONATINE INSENSITIVE1 (COI1) is involved in JA-signaling. The ETHYLENE INSENSITIVE2 (EIN2) protein activates EIN3/EIL1 in ET-presence. To understand whether EIN2 was also involved, the AR-response of ein2-1 and coi1-16 TCLs was evaluated adding the ET-precursor 1-aminocyclopropane1-carboxylic acid (ACC, 0.1 μM) and/or the JA-donor methyl jasmonate (JAMe, 0.01 μM) to IBA+vitamins-containing medium. AR-formation was enhanced by JAMe, reduced by ACC, but unchanged by JAMe+ACC in the wild type TCLs, whereas remained similarly low in ein2-1 and coi1-16 under all treatments. Collectively, these results demonstrate that the antagonism between JA and ET in AR-formation from AtTCLs involves a cross-talk by EIN2 and COI1

Sulfur Metabolism and Sulfur-Containing Amino Acids Derivatives – II: Autism Spectrum Disorders, Schizophrenia and Fibromyalgia

The metabolism of sulfur (S) compounds concurs to the maintain of cell homeostasis and tissue integrity in the human body. Sulfur chemical species act in all cells as anti-oxidant/scavenging agents or regulators of membrane stability/excitability. At the same time, they also exert tissue-dependent functions behaving as protective molecules of the liver and cardiovascular system, as modulators of the immune response, gut activity and CNS neurotransmitter signaling. The involvement of S compounds in human complex, chronic, disabling diseases at multifaceted pathogenesis is actually under investigation: altered levels of S metabolites could be in fact bio-indicators of impaired oxidation state in the body and their unbalance could be risk factors for disease onset. By the present review, we will discuss data from the literature which unearth an altered S biochemistry in human complex illnesses, taking as an example highly invalidating neuropsychiatry and pain perception diseases as autism spectrum disorders (ASD), schizophrenia and fibromyalgia. As well, we will depict herein the utility at applying to this area of the clinical research high resolving -omics strategies in combination with methodological tools which specifically explore S metabolism in patients. The perspectives of these kind of analyses would be the adoption of more valuable, personalized therapeutics protocols and, possibly, an improved bio-monitoring of patients, also including their response to treatments

Tryptophan Biochemistry: Structural, Nutritional, Metabolic, and Medical Aspects in Humans

L-Tryptophan is the unique protein amino acid (AA) bearing an indole ring: its biotransformation in living organisms contributes either to keeping this chemical group in cells and tissues or to breaking it, by generating in both cases a variety of bioactivemolecules. Investigations on the biology of Trp highlight the pleiotropic effects of its small derivatives on homeostasis processes. In addition to protein turn-over, in humans the pathways of Trp indole derivatives cover the synthesis of the neurotransmitter/hormone serotonin (5-HT), the pineal gland melatonin (MLT), and the trace amine tryptamine. The breakdown of the Trp indole ring defines instead the “kynurenine shunt” which produces cell-response adapters as L-kynurenine, kynurenic and quinolinic acids, or the coenzyme nicotinamide adenine dinucleotide (NAD+). This reviewaims therefore at tracing a “map” of themainmolecular effectors in human tryptophan (Trp) research, starting from the chemistry of this AA, dealing then with its biosphere distribution and nutritional value for humans, also focusing on some proteins responsible for its tissue-dependent uptake and biotransformation.We will thus underscore the role of Trp biochemistry in the pathogenesis of human complex diseases/syndromes primarily involving the gut, neuroimmunoendocrine/stress responses, and the CNS, supporting the use of -Omics approaches in this field

Parkinson’s Disease and Alpha-Synucleinopathies: from Arising Pathways to Therapeutic Challenge

Parkinson’s Disease (PD) and alpha synucleinopathies are multifactorial disorders, which manifest through motor symptoms and non-motor symptoms involving the Central Nervous System (CNS), the Peripheral Nervous System (PNS) and, recently, also the Enteric Nervous System (ENS). The typical hallmarks of alpha synucleinopathies are proteinaceous inclusions of alpha synuclein (αS). In PD they are known as Lewy Bodies (LBs) and Lewy Neurites (LNs), discovered in dopaminergic neurons of substantia nigra (pars compacta) as well as in other regions of the central and peripheral nervous systems. Despite the clear causes which lead to LBs/LNs are still unknown, according to Braak’s theory, these inclusions appear first in PNS to spread, following neuronal innervation, towards the CNS in a spatio- temporal dissemination described in a staging procedure. In line with these observations, several animal models have been used with the purpose to reproduce PD as well as to propose new therapeutic approaches. Different pathways can cooperate to neurodegeneration in PD such as genetic mutations of αS gene, mitochondrial dysfunctions, neuroinflammation. The present review highlights αS as the key-word for PD pathology and alpha synucleinopathies and a main target in PD research. Several therapeutic approaches can be proposed, however all of them are addressed in advanced stages of the pathology. Our focus will be the alteration of αS physiological pathway, which allows to address therapy in early stages at intracellular or extracellular level, such as the use of anti ER-stress compounds and innovative immunotherapy, which could be promising tools to reduce neuronal degeneration and to halt PD progression

User indoor localisation system enhances activity recognition: A proof of concept

Older people would like to live independently in their home as long as possible. They want to reduce the risk of domestic accidents because of polypharmacy, physical weakness and other mental illnesses, which could increase the risks of domestic accidents (i.e. a fall). Changes in the behaviour of healthy older people could be correlated with cognitive disorders; consequently, early intervention could delay the deterioration of the disease. Over the last few years, activity recognition systems have been developed to support the management of senior citizensâ\u80\u99 daily life. In this context, this paper aims to go beyond the state-of-the-art presenting a proof of concept where information on body movement, vital signs and userâ\u80\u99s indoor locations are aggregated to improve the activity recognition task. The presented system has been tested in a realistic environment with three users in order to assess the feasibility of the proposed method. These results encouraged the use of this approach in activity recognition applications; indeed, the overall accuracy values, amongst others, are satisfactory increased (+2.67% DT, +7.39% SVM, +147.37% NN)

Desarrollo larval de Etropus longimanus (Paralichthyidae) y Symphurus trewavasae (Cynoglossidae) en la costa Bonaerense, Argentina

The larval development of Etropus longimanus and Symphurus trewavasae (Pleuronectiformes) off the Buenos Aires coast was described. Both species have an elongated body; however, as the total length of Etropus longimanus larvae increased, their body became deeper. In Symphurus trewavasae the intestine was noticeably coiled. In E. longimanus the notochord flexion started at 3.9 mm and was completed at 5.0 mm standard length (SL). Vertebral formation began in larvae with a 4.6 mm SL and the definitive number of vertebrae (34-39) was observed in larvae of 4.8 mm SL. The dorsal fin had two elongated rays and the pelvic fins had only one. In Symphurus trewavasae the notochord flexion began at 5.9 mm and was completed at 8.0 mm SL. Migration of the right eye was completed in the metamorphic stage at 10.5 mm SL. Vertebral column ossification finished in flexion larvae of 6-7 mm SL, with a total number of 48-50 vertebrae. Four elongated rays of similar length were observed on the dorsal fin.Se describe el desarrollo larvario de Etropus longimanus y Symphurus trewavasae (Pleuronectiformes) que habitan la costa de Buenos Aires. El cuerpo de las larvas de ambas especies es alargado, pero en Etropus longimanus la altura del cuerpo se incrementa con el crecimiento. El tubo digestivo de Symphurus trewavasae es considerablemente enrollado. La flexión del urostilo en larvas de E. longimanus se inició cerca de los 3.9 mm y finalizó cerca de los 5 mm de longitud estándar (LS). Se observaron vértebras definidas en larvas de 4.6 mm LS y el número definitivo (34-39) se visualizó en individuos de 4.8 mm LS. Se distinguen dos radios largos al inicio de la aleta dorsal y uno en la pélvica. En Symphurus trewavasae la flexión del urostilo comenzó cerca de los 5.9 mm LS y finalizó aproximadamente a los 8.0 mm LS. La migración del ojo derecho hacia el lado izquierdo de la cabeza finalizó en el estadio de metamorfosis (10.5 mm LS). La osificación de la columna vertebral se completó en larvas entre los 6 y 7 mm LS, con un número total de 48-59 vértebras. Al inicio de la aleta dorsal se distinguen 4 radios largos con igual longitud

Jasmonate and nitric oxide roles in the control of xylary cell formation and identity in Arabidopsis seedlings

In basal hypocotyls of dark-grown Arabidopsis seedlings, xylary cells may form from the pericycle as an alternative to another developmental program, i.e. adventitious roots. It is known that several hormones may induce xylogenesis, as jasmonic acid (JA), indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA), which also affect xylary cell identity. Recent studies with the ethylene (ET)-perception mutant ein3eil1 and the ET-precursor 1-aminocyclopropane-1-carboxylic acid (ACC) have shown ET involvement in IBA induced ectopic metaxylem. Nitric oxide (NO) is a reactive free radical molecule, which acts as a messenger in several cell differentiation events, including programmed cell death, moreover it can be produced after IBA/IAA-treatments influencing JA signalling and interacting positively/negatively with ET. To date, NO involvement in ET/JA-mediated xylogenesis has never been investigated.The aim of the present research was to determine the involvement of JA, ET and NO in the control of endogenous/exogenous auxin-induced xylogenesis through a possible crosstalk mediated by EIN3/EIL1. To this aim, ectopic xylem formation was investigated in the hypocotyl of dark-grown Arabidopsis seedlings exposed to various concentrations of JA methyl-ester (JAMe) with/without ACC, IBA or IAA. The xylogenic response in the wild-type (wt) was compared with that of the ein3eil1 mutant, the NO signal was quantified and the its role evaluated by measuring the effects of treatments with a NO donor/scavenger (SNP/cPTIO). Results show that the ectopic formation of protoxylem was enhanced in the wt by JAMe when applied alone at a specific concentration (i.e. 10μM), whereas in ein3eil1 mutant it occurred with any JAMe concentration (i.e. 0.01, 1 and 10 μM). This stimulation of xylary elements mediated by JAMe suggests that a negative interaction between JA and ET-signalling is involved in this developmental program. The negative interaction was confirmed by the reduction in xylogenesis observed in the wt after the combined application of JAMe with ACC, in comparison with JAMe alone. Nitric oxide was detected at early stages of both xylogenesis and adventitious rooting in the hypocotyl pericycle cells and its production was highly enhanced by JAMe at the highest concentration, combined or not with IBA (10 μM). Histological analyses showed that the xylary identity changed when JAMe was applied with each auxin in comparison with treatments with auxin alone. In addition, the IBA/IAA-induced adventitious rooting was increased by the same JAMe concentration enhancing xylogenesis when applied alone. This suggests a role for JA in modulating both developmental programs (adventitious rooting and xylogenesis) in the same target cells (hypocotyl pericycle cells), through an interaction with NO, as summarized in the model proposed (Fig. 1)

Jasmonates, ethylene and brassinosteroids control adventitious and lateral rooting as stress avoidance responses to heavy metals and metalloids

Developmental and environmental signaling networks often converge during plant growth in response to changing conditions. Stress-induced hormones, such as jasmonates (JAs), can influence growth by crosstalk with other signals like brassinosteroids (BRs) and ethylene (ET). Nevertheless, it is unclear how avoidance of an abiotic stress triggers local changes in development as a response. It is known that stress hormones like JAs/ET and BRs can regulate the division rate of cells from the first asymmetric cell divisions (ACDs) in meristems, suggesting that stem cell activation may take part in developmental changes as a stress-avoidance-induced response. The root system is a prime responder to stress conditions in soil. Together with the primary root and lateral roots (LRs), adventitious roots (ARs) are necessary for survival in numerous plant species. AR and LR formation is affected by soil pollution, causing substantial root architecture changes by either depressing or enhancing rooting as a stress avoidance/survival response. Here, a detailed overview of the crosstalk between JAs, ET, BRs, and the stress mediator nitric oxide (NO) in auxin-induced AR and LR formation, with/without cadmium and arsenic, is presented. Interactions essential in achieving a balance between growth and adaptation to Cd and As soil pollution to ensure survival are reviewed here in the model species Arabidopsis and rice

Alteration of serotonin transporter density and activity in fibromyalgia

The aim of the study was to evaluate the kinetic parameters of a specific serotonin transporter (SERT) and serotonin uptake in a mentally healthy subset of patients with fibromyalgia. Platelets were obtained from 40 patients and 38 healthy controls. SERT expression and functionality were evaluated through the measurement of [(3)H]paroxetine binding and the [(3)H]serotonin uptake itself. The values of maximal membrane binding capacity (B(max)) were statistically lower in the patients than in the healthy volunteers, whereas the dissociation constant (K(d)) did not show any statistically significant variations. Moreover, a decrease in the maximal uptake rate of SERT (V(max)) was demonstrated in the platelets of patients, whereas the Michaelis constant (K(m)) did not show any statistically significant variations. Symptom severity score (tiredness, tender points index and Fibromyalgia Impact Questionnaire) were negatively correlated with B(max )and with V(max), and positively correlated with K(m). A change in SERT seems to occur in fibromyalgic patients, and it seems to be related to the severity of fibromyalgic symptoms

Costruire competenze progettuali e valutative attraverso la didattica laboratoriale

The achievement of essential skills for teaching professions is a key element in the educational policies of many countries and it also represents an important topic for national and international research in education. Within the framework of Primary Teacher Education degree (University of Bologna), the authors have started a research path focused on laboratory’s teaching effectiveness to promote planning and evaluation skills of future teachers, in a constructivistic perspective. At the moment, we came to the end of the first phase, exploratory and qualitative, that was conducted in a workshop of the second year of the course. Such a phase allowed us to define the ways and tools to investigate teacher expertise in a tutorial. At the same time this outlined the need of integrating experience based strategies with the following practicum, in a longitudinal perspectiveL’acquisizione delle competenze essenziali per la professionalità docente è un elemento centrale nelle politiche educative di molti paesi ed è anche al centro di un interessante filone della ricerca pedagogica nazionale e internazionale. Nell’ambito del Corso di Laurea in Scienze della Formazione Primaria dell’Università di Bologna, si é dato avvio a un percorso di ricerca avente come oggetto esperienze formative laboratoriali e progettate privilegiando una didattica costruttivistica, con l’obiettivo di promuovere la formazione delle competenze di progettazione e valutazione nei futuri insegnanti. Al momento si è giunti al termine di una prima fase, di tipo esplorativo-qualitativo, realizzata all’interno di un laboratorio del secondo anno di corso che, da una parte, ha permesso di definire le modalità e gli strumenti per indagare queste competenze nell’ambito del laboratorio specifico e, dall’altra, ha reso evidente la necessità di articolare tutte le istanze formative di tipo esperienziale e in particolare collegare, in una prospettiva di tipo longitudinale, il laboratorio con il tirocinio curricolare