Degradation of Potassium Rock by Earthworms and Responses of Bacterial Communities in Its Gut and Surrounding Substrates after Being Fed with Mineral

BACKGROUND: Earthworms are an ecosystem's engineers, contributing to a wide range of nutrient cycling and geochemical processes in the ecosystem. Their activities can increase rates of silicate mineral weathering. Their intestinal microbes usually are thought to be one of the key drivers of mineral degradation mediated by earthworms,but the diversities of the intestinal microorganisms which were relevant with mineral weathering are unclear. METHODOLOGY/PRINCIPAL FINDINGS: In this report, we show earthworms' effect on silicate mineral weathering and the responses of bacterial communities in their gut and surrounding substrates after being fed with potassium-bearing rock powder (PBRP). Determination of water-soluble and HNO(3)-extractable elements indicated some elements such as Al, Fe and Ca were significantly released from mineral upon the digestion of earthworms. The microbial communities in earthworms' gut and the surrounding substrates were investigated by amplified ribosomal DNA restriction analysis (ARDRA) and the results showed a higher bacterial diversity in the guts of the earthworms fed with PBRP and the PBRP after being fed to earthworms. UPGMA dendrogram with unweighted UniFrac analysis, considering only taxa that are present, revealed that earthworms' gut and their surrounding substrate shared similar microbiota. UPGMA dendrogram with weighted UniFrac, considering the relative abundance of microbial lineages, showed the two samples from surrounding substrate and the two samples from earthworms' gut had similarity in microbial community, respectively. CONCLUSIONS/SIGNIFICANCE: Our results indicated earthworms can accelerate degradation of silicate mineral. Earthworms play an important role in ecosystem processe since they not only have some positive effects on soil structure, but also promote nutrient cycling of ecosystem by enhancing the weathering of minerals

Beneficial effects of reading aloud and solving simple arithmetic calculations (learning therapy) on a wide range of cognitive functions in the healthy elderly: study protocol for a randomized controlled trial

<p>Abstract</p> <p>Background</p> <p>Almost all cognitive functions decline with age. Results of previous studies have shown that cognitive training related to everyday life (reading aloud and solving simple arithmetic calculations), namely learning therapy, can improve two cognitive function (executive functions and processing speed) in elderly people. However, it remains unclear whether learning therapy engenders improvement of various cognitive functions or not. We investigate the impact of learning therapy on various cognitive functions (executive functions, episodic memory, short-term memory, working memory, attention, reading ability, and processing speed) in healthy older adults.</p> <p>Methods</p> <p>We use a single-blinded intervention with two parallel groups (a learning therapy group and a waiting list control group). Testers are blind to the study hypothesis and the group membership of participants. Through an advertisement in local newspaper, 64 healthy older adults are recruited. They will be assigned randomly to a learning therapy group or a waiting list control group. In the learning therapy group, participants are required to perform two cognitive tasks for 6 months: reading Japanese aloud and solving simple calculations. The waiting list group does not participate in the intervention. The primary outcome measure is the Stroop test score: a measure of executive function. Secondary outcome measures are assessments including the following: verbal fluency task, logical memory, first and second names, digit span forward, digit span backward, Japanese reading test, digit cancellation task, digit symbol coding, and symbol search. We assess these outcome measures before and after the intervention.</p> <p>Discussion</p> <p>This report is the first study which investigates the beneficial effects of learning therapy on a wide range of cognitive functions of elderly people. Our study provides sufficient evidence of learning therapy effectiveness. Most cognitive functions, which are correlated strongly with daily life activities, decrease with age. These study results can elucidate effects of cognitive training on elderly people.</p> <p>Trial registration</p> <p>This trial was registered in The University Hospital Medical Information Network Clinical Trials Registry (No. <a href="http://www.clinicaltrials.gov/ct2/show/UMIN000006998">UMIN000006998</a>).</p

Inhibition of Lassa Virus Glycoprotein Cleavage and Multicycle Replication by Site 1 Protease-Adapted α1-Antitrypsin Variants

The virus family Arenaviridae includes several hemorrhagic fever causing agents such as Lassa, Guanarito, Junin, Machupo, and Sabia virus that pose a major public health concern to the human population in West African and South American countries. Current treatment options to control fatal outcome of disease are limited to the ribonucleoside analogue ribavirin, although its use has some significant limitations. The lack of effective treatment alternatives emphasizes the need for novel antiviral therapeutics to counteract these life-threatening infections. Maturation cleavage of the viral envelope glycoprotein by the host cell proprotein convertase site 1 protease (S1P) is critical for infectious virion production of several pathogenic arenaviruses. This finding makes this protease an attractive target for the development of novel anti-arenaviral therapeutics. We demonstrate here that highly selective S1P-adapted α1-antitrypsins have the potential to efficiently inhibit glycoprotein processing, which resulted in reduced Lassa virus replication. Our findings suggest that S1P should be considered as an antiviral target and that further optimization of modified α1-antitrypsins could lead to potent and specific S1P inhibitors with the potential for treatment of certain viral hemorrhagic fevers

Dietary phytochemicals, HDAC inhibition, and DNA damage/repair defects in cancer cells

Genomic instability is a common feature of cancer etiology. This provides an avenue for therapeutic intervention, since cancer cells are more susceptible than normal cells to DNA damaging agents. However, there is growing evidence that the epigenetic mechanisms that impact DNA methylation and histone status also contribute to genomic instability. The DNA damage response, for example, is modulated by the acetylation status of histone and non-histone proteins, and by the opposing activities of histone acetyltransferase and histone deacetylase (HDAC) enzymes. Many HDACs overexpressed in cancer cells have been implicated in protecting such cells from genotoxic insults. Thus, HDAC inhibitors, in addition to unsilencing tumor suppressor genes, also can silence DNA repair pathways, inactivate non-histone proteins that are required for DNA stability, and induce reactive oxygen species and DNA double-strand breaks. This review summarizes how dietary phytochemicals that affect the epigenome also can trigger DNA damage and repair mechanisms. Where such data is available, examples are cited from studies in vitro and in vivo of polyphenols, organosulfur/organoselenium compounds, indoles, sesquiterpene lactones, and miscellaneous agents such as anacardic acid. Finally, by virtue of their genetic and epigenetic mechanisms, cancer chemopreventive agents are being redefined as chemo- or radio-sensitizers. A sustained DNA damage response coupled with insufficient repair may be a pivotal mechanism for apoptosis induction in cancer cells exposed to dietary phytochemicals. Future research, including appropriate clinical investigation, should clarify these emerging concepts in the context of both genetic and epigenetic mechanisms dysregulated in cancer, and the pros and cons of specific dietary intervention strategies

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Not AvailableIn this study, value added compost was prepared from low-grade Indian rock phosphates (RPs) and isabgol straw as an alternative sources of phosphorus. Three different sources of low-grade RP namely Udaipur, Jhabua and Purulia were evaluated for alternative source of P. Total carbon (C) decreased while total nitrogen (N) increased, leading to a decrease in the C/N ratio at the end of composting. Notable increases in total and available P was found in the composts charged with RPs. The rate of P released from the composts was studied through leaching experiment in the laboratory. An incubation study was carried out to evaluate the effect of different compost on soil P availability. Data revealed that the sharp increase in water soluble P from all the compost at 14 days of leaching then a gradual decrease. Significantly higher water soluble P released from the value added (RP charged) compost as compared to ordinary compost throughout the leaching period. Application of value added compost in soil increased water soluble, available P, microbial biomass C and P as well as acid and alkaline phosphatase activity in soil. Initially, the value added compost showed lower available P as compared to DAP however, improved significantly at a later stage of incubation. The increase in different labile pools of P in soil amended with value added compost also indicates substantial amount of P mobilized from the compost during incubation. Thus, the value added compost could be an effective alternative of chemical fertilizer and promising technology to utilize low-grade RP as P source.ICA

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Not AvailableAn attempt was made to recycle waste biomass and mineral powder (waste mica) as an alternative source of potassium (K) through composting technology. Two different waste biomass, isabgol straw and palmarosa distillation waste along with two levels of waste mica (2 and 4% as K) were used for preparation of enriched composts. A notable decrease of C:N ratio was observed at the end of the composting (150 days) as an indicator of compost maturity. The mature composts were evaluated for K-supplying capacity through laboratory leaching and soil incubation study. Significantly higher water-soluble K released initially followed by a sharp decrease up to 21 days of leaching thereafter gradually decreased up to 35 days of leaching. Water-soluble K was released from K-enriched (mica charged) compost significantly higher than the ordinary compost throughout the leaching period. Soil incubation study also revealed that application of K-enriched compost greatly improved the available K (water soluble and exchangeable) pools in K-deficient soil which indicated that a considerable amount of K releases during composting. Therefore, K-enriched compost could be an effective alternative of costly commercial K fertilizer and eco-friendly approach to utilize low-cost waste mineral powder and plant residue.ICA

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Not AvailableMost of the Indian rock phosphates (RPs) are not suitable for production of commercial phosphate fertilizer because of their low phosphorus (P) content (low-grade). They are suitable as source of P in acid soil, but not effective for direct use in neutral to alkaline soil. The main aim of this study was to evaluate the potentiality of these RPs as liming material and subsequently their effect on P availability in acid soil. Four Indian RPs namely, Udaipur, Mussoorie, Jhabua and Purulia were evaluated for their liming potential through theoretical calculations, laboratory titration and soil incubation studies. The incubation experiment was carried out for 90 days to quantify more accurate per cent calcium carbonate equivalence (%CCE) values of RPs as well as the changes in pH, exchangeable aluminum (Al) and P availability in an acid soil having pH 4.53. Results emanated from the theoretical calculation showed that the %CCE of Indian RPs varied from 59 to 62. While, laboratory studies on quantification of %CCE (AOAC method 955.01) of RPs by titration ranged between 39.9 and 53.7 which were lesser than the theoretical values. The %CCE values obtained in soil incubation study at lower rate of RP application followed the similar pattern as those values obtained in the theoretical calculation. Though RP was less effective in increasing soil pH as compared to CaCO3, there was an increase in soil pH due to application of RPs, which is sufficient enough to decrease the content of Al much below the toxic and safe limit for supplying available P for plant growth. Thus, application of low-grade RPs could be a potential option as liming material in acid soil in addition to a source of P for crop production.ICA

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Not AvailableA pot culture experiment was conducted for two consecutive years in the late rainy season to evaluate the efficiency of rock phosphate charged compost as an organic P fertilizer source in senna. A fixed dose of P (40 kg ha−1), considered optimum for quality produce was supplied from different sources like Di-ammonium phosphate (DAP), Single Super Phosphate (SSP), Rock phosphate (RP) and RP charged compost (RPCC). Fresh and dry weight of leaves and pods per plant were recorded while sennoside content in leaves and pods samples were analyzed. Total fresh and dry herbage yield (leaf + pod) was significantly higher under different P treatments than the control. Application of RP, SSP, DAP and RPCC increased fresh herbage yield up to 6.7, 22.6, 35.1 and 48.1%, respectively over the control. Sennoside content did not respond to all P sources, but application of RPCC improved sennoside content in herbage. The highest herbage yield and sennoside content was recorded under RPCC application. Available P and microbial biomass P in soil after harvesting of crop was significantly higher under RPCC application but on par with DAP application. Both acid and alkaline phosphatase activities in soil were influenced by P sources, while alkaline phosphatase found significantly higher with RPCC application. So, it can be concluded from the study that enriched compost effectively supplement costly chemical fertilizer like DAP as a P source. The result from the study may be recommended for efficient P management in both commercial as well as organic cultivation of senna.ICA