91 research outputs found

    High-throughput screening of monoclonal antibodies against plant cell wall glycans by hierarchical clustering of their carbohydrate microarray binding profiles

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    Antibody-producing hybridoma cell lines were created following immunisation with a crude extract of cell wall polymers from the plant Arabidopsis thaliana. In order to rapidly screen the specificities of individual monoclonal antibodies (mAbs), their binding to microarrays containing 50 cell wall glycans immobilized on nitrocellulose was assessed. Hierarchical clustering of microarray binding profiles from newly produced mAbs, together with the profiles for mAbs with previously defined specificities allowed the rapid assignments of mAb binding to antigen classes. mAb specificities were further investigated using subsequent immunochemical and biochemical analyses and two novel mAbs are described in detail. mAb LM13 binds to an arabinanase-sensitive pectic epitope and mAb LM14, binds to an epitope occurring on arabinogalactan-proteins. Both mAbs display novel patterns of recognition of cell walls in plant materials

    A century of trends in adult human height

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    Being taller is associated with enhanced longevity, and higher education and earnings. We reanalysed 1472 population-based studies, with measurement of height on more than 18.6 million participants to estimate mean height for people born between 1896 and 1996 in 200 countries. The largest gain in adult height over the past century has occurred in South Korean women and Iranian men, who became 20.2 cm (95% credible interval 17.5-22.7) and 16.5 cm (13.3-19.7) taller, respectively. In contrast, there was little change in adult height in some sub-Saharan African countries and in South Asia over the century of analysis. The tallest people over these 100 years are men born in the Netherlands in the last quarter of 20th century, whose average heights surpassed 182.5 cm, and the shortest were women born in Guatemala in 1896 (140.3 cm; 135.8-144.8). The height differential between the tallest and shortest populations was 19-20 cm a century ago, and has remained the same for women and increased for men a century later despite substantial changes in the ranking of countries

    Absorption systems in the spectrum of GRB 021004

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    Original article can be found at: http://www.aanda.org/--Copyright The European Southern Observatory (ESO) DOI : 10.1051/0004-6361:20021612We report on a 3600 s spectrum of GRB 021004 obtained with the Nordic Optical Telecope on La Palma 10.71 hours after the burst. We identify absorption lines from five systems at redshifts 1.3806, 1.6039, 2.2983, 2.3230, and 2.3292. In addition we find an emission line which, if due to Ly from the host galaxy, gives a redshift of 2.3351. The nearest absorber is blueshifted by 530 km s−1 with respect to this line, consistent with shifts seen in Damped Ly and Lyman-Break galaxies at similar redshifts. The emission line flux is 2:46 0:50 10−16 erg s−1 cm−2. Some of the absorption systems are “line-locked”, an effect often seen in QSO absorption systems believed to originate close to the QSO central engine.Peer reviewe
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