Characterization of an endogenous gene expressed in Aedes aegypti using an orally infectious recombinant Sindbis virus

Sindbis virus expression vectors have been used successfully to express and silence genes of interest in vivo in several mosquito species, including Aedes aegypti, Ae. albopictus, Ae. triseriatus,Culex pipiens, Armigeres subalbatus and Anopheles gambiae. Here we describe the expression of an endogenous gene, defensin, in Ae. aegypti using the orally infectious Sindbis virus, MRE/3′2J expression vector. We optimized conditions to infect mosquito larvae per os using C6/36 Ae. albopictus cells infected with the recombinant virus to maximize virus infection and expression of defensin. Infection with the parental Sindbis virus (MRE/3′2J) did not induce defensin expression. Mosquito larvae infected by ingestion of recombinant Sindbis virus-infected C6/36 cells expressed defensin when they emerged as adults. Defensin expression was observed by western analysis or indirect fluorescent assay in all developmental stages of mosquitoes infected with MRE/3′2J virus that contained the defensin insert. The multiplicity of infection of C6/36 cells and the quantity of infected cells consumed by larvae played an important role in defensin expression. Parental viruses, missing the defensin insert, and/or other defective interfering virus may have contributed to these observations

Calcium, vitamin D, casein and whey protein intakes and periodontitis among Danish adults

Objective: To investigate whether intakes of Ca, vitamin D, casein and whey are associated with periodontitis and to investigate the possibility of interactions between them. Design: Cross-sectional study. An Internet-based, 267-item FFQ was used to assess dietary intake. Intakes of casein (32·0 g/d), whey proteins (9·6 g/d) and vitamin D (5·8 μg/d) were classified as within v. above the 50th percentile. Ca intake was classified as within v. below age-specific recommendations. Severe periodontitis was defined as having ≥2 inter-proximal sites with clinical attachment loss ≥6 mm (not on the same tooth) and ≥1 inter-proximal site with pocket depth ≥5 mm. Since vitamin D influences Ca absorption, models were stratified by lower and higher (<5·8 v. ≥5·8 µg/d) vitamin D intake. Setting: Danish Health Examination Survey (DANHES) 2007–2008. Subjects: Adult participants (n 3287) in the oral health study of DANHES 2007–2008. Results: Intakes of Ca within recommendations (OR=0·76; 95 % CI 0·58, 0·99), whey ≥9·6 g/d (OR=0·75; 95 % CI 0·58, 0·97) and casein ≥32 g/d (OR=0·75 95 % CI 0·58, 0·97) were associated with lower likelihood of severe periodontitis after adjustment for age, gender, education, smoking, sucrose intake, alcohol consumption, number of teeth, daily brushing, regular visits to the dentist and chronic illness, irrespective of vitamin D intake levels. Intake of vitamin D alone was not associated severe with periodontitis. Conclusions: Intakes of Ca, casein and whey protein were inversely associated with periodontitis. Consumption of foods rich in Ca, casein and whey (e.g. dairy foods) should be promoted, as they may contribute to the prevention of periodontitis. Further longitudinal studies are required to confirm these associations

Polyakov Loops versus Hadronic States

The order parameter for the pure Yang-Mills phase transition is the Polyakov loop which encodes the symmetries of the Z_N center of the SU(N) gauge group. On the other side the physical degrees of freedom of any asymptotically free gauge theory are hadronic states. Using the Yang-Mills trace anomaly and the exact Z_N symmetry we construct a model able to communicate to the hadrons the information carried by the order parameter.Comment: RevTex4 2-col., 6 pages, 2 figures. Typos fixed and added a paragraph in the conclusion

Partial Deconfinement in Color Superconductivity

We analyze the fate of the unbroken SU(2) color gauge interactions for 2 light flavors color superconductivity at non zero temperature. Using a simple model we compute the deconfining/confining critical temperature and show that is smaller than the critical temperature for the onset of the superconductive state itself. The breaking of Lorentz invariance, induced already at zero temperature by the quark chemical potential, is shown to heavily affect the value of the critical temperature and all of the relevant features related to the deconfining transition. Modifying the Polyakov loop model to describe the SU(2) immersed in the diquark medium we argue that the deconfinement transition is second order. Having constructed part of the equation of state for the 2 color superconducting phase at low temperatures our results are relevant for the physics of compact objects featuring a two flavor color superconductive state.Comment: 9 pp, 4 eps-figs, version to appear in PR

Clay content drives carbon stocks in soils under a plantation of Eucalyptus saligna Labill. in southern Brazil

Soil carbon accumulation is largely dependent on net primary productivity. To our knowledge, there have been no studies investigating the dynamics of carbon accumulation in weathered subtropical soils, especially in managed eucalyptus plantations. We quantified the seasonal input of leaf litter, the leaf decomposition rate and soil carbon stocks in an commercial plantation of Eucalyptus saligna Labill. in southern Brazil. Our goal was to evaluate, through multiple linear regression, the influence that certain chemical characteristics of litter, as well as chemical and physical characteristics of soil, have on carbon accumulation in soil organic matter fractions. Variables related to the chemical composition of litter were not associated with the soil carbon stock in the particulate and mineral fractions. However, certain soil characteristics were significantly associated with the carbon stock in both fractions. The concentrations of nutrients associated with plant growth and productivity, such as phosphorus, sulfur, copper and zinc, were associated with variations in the labile carbon pool (particulate fraction). Clay content was strongly associated with the carbon stock in the mineral fraction. The carbon accumulation and stabilization in weathered subtropical Ultisol seems to be mainly associated with the intrinsic characteristics of the soil, particularly clay content, rather than with the quantity, chemical composition or decomposition rate of the litter

The Sensitivity of Ligo to a Stochastic Background, and its Dependance on the Detector Orientations

We analyze the sensitivity of a network of interferometer gravitational-wave detectors to the gravitational-wave stochastic background, and derive the dependence of this sensitivity on the orientations of the detector arms. We build on and extend the recent work of Christensen, but our conclusion for the optimal choice of orientations of a pair of detectors differs from his. For a pair of detectors (such as LIGO) that subtends an angle at the center of the earth of \,\alt 70^\circ, we find that the optimal configuration is for each detector to have its arms make an angle of $45^\circ$ (modulo $90^\circ$) with the arc of the great circle that joins them. For detectors that are farther separated, each detector should instead have one arm aligned with this arc. We also describe in detail the optimal data-analysis algorithm for searching for the stochastic background with a detector network, which is implicit in earlier work of Michelson. The LIGO pair of detectors will be separated by $\sim 3000 \, {\rm km}$. The minimum detectable stochastic energy-density for these detectors with their currently planned orientations is $\sim 3\%$ greater than what it would be if the orientations were optimal.Comment: 56 pages, 10 figures, Caltech preprint GRP-347, submitted to Phys Rev D, uses revtex macro

Detector Description and Performance for the First Coincidence Observations between LIGO and GEO

For 17 days in August and September 2002, the LIGO and GEO interferometer gravitational wave detectors were operated in coincidence to produce their first data for scientific analysis. Although the detectors were still far from their design sensitivity levels, the data can be used to place better upper limits on the flux of gravitational waves incident on the earth than previous direct measurements. This paper describes the instruments and the data in some detail, as a companion to analysis papers based on the first data.Comment: 41 pages, 9 figures 17 Sept 03: author list amended, minor editorial change