Molecular mechanisms underlying differential odor responses of a mouse olfactory receptor

The prevailing paradigm for G protein-coupled receptors is that each receptor is narrowly tuned to its ligand and closely related agonists. An outstanding problem is whether this paradigm applies to olfactory receptor (ORs), which is the largest gene family in the genome, in which each of 1,000 different G protein-coupled receptors is believed to interact with a range of different odor molecules from the many thousands that comprise “odor space.” Insights into how these interactions occur are essential for understanding the sense of smell. Key questions are: (i) Is there a binding pocket? (ii) Which amino acid residues in the binding pocket contribute to peak affinities? (iii) How do affinities change with changes in agonist structure? To approach these questions, we have combined single-cell PCR results [Malnic, B., Hirono, J., Sato, T. & Buck, L. B. (1999) Cell 96, 713–723] and well-established molecular dynamics methods to model the structure of a specific OR (OR S25) and its interactions with 24 odor compounds. This receptor structure not only points to a likely odor-binding site but also independently predicts the two compounds that experimentally best activate OR S25. The results provide a mechanistic model for olfactory transduction at the molecular level and show how the basic G protein-coupled receptor template is adapted for encoding the enormous odor space. This combined approach can significantly enhance the identification of ligands for the many members of the OR family and also may shed light on other protein families that exhibit broad specificities, such as chemokine receptors and P450 oxidases

HI Observations of the Stephan's Quintet

Using the VLA, we have made spectral-line and continuum observations of the neutral hydrogen in the direction of the compact group of galaxies Stephan's Quintet. The high-velocity clouds between 5600 and 6600 km/s, the disk of the foreground galaxy, NGC 7320, at 800 km/s, the extended continuum ridge near the center of the group, and 3 faint dwarf-like galaxies in the surrounding field were imaged with C, CS, and D arrays. Four of the HI clouds previously detected are confirmed. The two largest HI features are coincident with and concentrated mainly along separate large tidal tails that extend eastward. The most diffuse of the four clouds is resolved into two clumps, one coincide with tidal features south of NGC 7318a and the other devoid of any detectable stellar or Halfa sources. The two compact clouds, along the same line of sight, have peak emission at luminous infrared and bright Halfa sources probably indicative of star-forming activity. The total amount of HI detected at high redshifts is ~ 10**10Msol. As in previous HI studies of the group, no detectable emission was measured at the positions of any high-redshift galaxies so that any HI still bound to their disks must be less than 2.4 x 10**7Msol.Comment: Accepted for publication in AJ and tentatively scheduled for the May 2002 issue. High-resolution ps figures are available at: http://www.iaa.es/~lourdes/bw/bw-paper.tar.g

Comparison of the Electronic Structures and Energetics of Ferroelectric LiNbO3 and LiTaO3

This paper explains the origin of the ferroelectric instability in LiNbO3 and LiTaO3 and compares the electronic structures and energetics of the two materials.Comment: 31 pages, 11 Postscript figure

Accretion of Planetary Material onto Host Stars

Accretion of planetary material onto host stars may occur throughout a star's life. Especially prone to accretion, extrasolar planets in short-period orbits, while relatively rare, constitute a significant fraction of the known population, and these planets are subject to dynamical and atmospheric influences that can drive significant mass loss. Theoretical models frame expectations regarding the rates and extent of this planetary accretion. For instance, tidal interactions between planets and stars may drive complete orbital decay during the main sequence. Many planets that survive their stars' main sequence lifetime will still be engulfed when the host stars become red giant stars. There is some observational evidence supporting these predictions, such as a dearth of close-in planets around fast stellar rotators, which is consistent with tidal spin-up and planet accretion. There remains no clear chemical evidence for pollution of the atmospheres of main sequence or red giant stars by planetary materials, but a wealth of evidence points to active accretion by white dwarfs. In this article, we review the current understanding of accretion of planetary material, from the pre- to the post-main sequence and beyond. The review begins with the astrophysical framework for that process and then considers accretion during various phases of a host star's life, during which the details of accretion vary, and the observational evidence for accretion during these phases.Comment: 18 pages, 5 figures (with some redacted), invited revie

Methods for specifying the target difference in a randomised controlled trial : the Difference ELicitation in TriAls (DELTA) systematic review

Peer reviewedPublisher PD

A Panchromatic View OF NGC 602: Time-Resolved Star Formation with the Hubble and Spitzer Space Telescopes

We present the photometric catalogs for the star-forming cluster NGC 602 in the wing of the Small Magellanic Cloud covering a range of wavelengths from optical HST/ACS (F555W, F814W) and SMARTS/ANDICAM (V, I) to infrared (Spitzer/IRAC 3.6, 4.5, 5.8, and 8 micron and MIPS 24 micron). Combining this with IRSF (InfraRed Survey Facility) near-infrared photometry (J, H, Ks), we compare the young main sequence (MS) and pre-main sequence (PMS) populations prominent in the optical with the current young stellar object (YSO) populations revealed by the infrared (IR). We analyze the MS and PMS population with isochrones in color-magnitude diagrams to derive ages and masses. The optical data reveal ~565 PMS candidates, low mass Stage III YSOs. We characterize ~40 YSOs by fitting their spectral energy distributions (SEDs) to a grid of models (Robitaille et al. 2007) to derive luminosities, masses and evolutionary phase (Stage I-III). The higher resolution HST images reveal that ~70% of the YSO candidates are either multiples or protoclusters. For YSOs and PMS sources found in common, we find a consistency in the masses derived. We use the YSO mass function to derive a present-day star-formation rate of ~0.2-1.0 Msun/yr/kpc^2, similar to the rate derived from the optical star formation history suggesting a constant star formation rate for this region. We demonstrate a progression of star formation from the optical star cluster center to the edge of the star forming dust cloud. We derive lifetimes of a few 10^5 years for the YSO Stages I and II.Comment: 55 pages, 18 Figures; High resolution images available form author Accepted to Ap

Planck 2013 results. XXII. Constraints on inflation

We analyse the implications of the Planck data for cosmic inflation. The Planck nominal mission temperature anisotropy measurements, combined with the WMAP large-angle polarization, constrain the scalar spectral index to be ns = 0:9603 _ 0:0073, ruling out exact scale invariance at over 5_: Planck establishes an upper bound on the tensor-to-scalar ratio of r < 0:11 (95% CL). The Planck data thus shrink the space of allowed standard inflationary models, preferring potentials with V00 < 0. Exponential potential models, the simplest hybrid inflationary models, and monomial potential models of degree n _ 2 do not provide a good fit to the data. Planck does not find statistically significant running of the scalar spectral index, obtaining dns=dln k = 0:0134 _ 0:0090. We verify these conclusions through a numerical analysis, which makes no slowroll approximation, and carry out a Bayesian parameter estimation and model-selection analysis for a number of inflationary models including monomial, natural, and hilltop potentials. For each model, we present the Planck constraints on the parameters of the potential and explore several possibilities for the post-inflationary entropy generation epoch, thus obtaining nontrivial data-driven constraints. We also present a direct reconstruction of the observable range of the inflaton potential. Unless a quartic term is allowed in the potential, we find results consistent with second-order slow-roll predictions. We also investigate whether the primordial power spectrum contains any features. We find that models with a parameterized oscillatory feature improve the fit by __2 e_ _ 10; however, Bayesian evidence does not prefer these models. We constrain several single-field inflation models with generalized Lagrangians by combining power spectrum data with Planck bounds on fNL. Planck constrains with unprecedented accuracy the amplitude and possible correlation (with the adiabatic mode) of non-decaying isocurvature fluctuations. The fractional primordial contributions of cold dark matter (CDM) isocurvature modes of the types expected in the curvaton and axion scenarios have upper bounds of 0.25% and 3.9% (95% CL), respectively. In models with arbitrarily correlated CDM or neutrino isocurvature modes, an anticorrelated isocurvature component can improve the _2 e_ by approximately 4 as a result of slightly lowering the theoretical prediction for the ` <_ 40 multipoles relative to the higher multipoles. Nonetheless, the data are consistent with adiabatic initial conditions