Scientific basis for safely shutting in the Macondo Well after the April 20, 2010 Deepwater Horizon blowout

As part of the government response to the Deepwater Horizon blowout, a Well Integrity Team evaluated the geologic hazards of shutting in the Macondo Well at the seafloor and determined the conditions under which it could safely be undertaken. Of particular concern was the possibility that, under the anticipated high shut-in pressures, oil could leak out of the well casing below the seafloor. Such a leak could lead to new geologic pathways for hydrocarbon release to the Gulf of Mexico. Evaluating this hazard required analyses of 2D and 3D seismic surveys, seafloor bathymetry, sediment properties, geophysical well logs, and drilling data to assess the geological, hydrological, and geomechanical conditions around the Macondo Well. After the well was successfully capped and shut in on July 15, 2010, a variety of monitoring activities were used to assess subsurface well integrity. These activities included acquisition of wellhead pressure data, marine multichannel seismic pro- files, seafloor and water-column sonar surveys, and wellhead visual/acoustic monitoring. These data showed that the Macondo Well was not leaking after shut in, and therefore, it could remain safely shut until reservoir pressures were suppressed (killed) with heavy drilling mud and the well was sealed with cement

Phosphorylation of Spinophilin Modulates Its Interaction with Actin Filaments

Spinophilin is a protein phosphatase 1 (PP1)- and actin-binding protein that modulates excitatory synaptic transmission and dendritic spine morphology. We report that spinophilin is phosphorylated in vitro by protein kinase A (PKA). Phosphorylation of spinophilin was stimulated by treatment of neostriatal neurons with a dopamine D1 receptor agonist or with forskolin, consistent with spinophilin being a substrate for PKA in intact cells. Using tryptic phosphopeptide mapping, site-directed mutagenesis, and microsequencing analysis, we identified two major sites of phosphorylation, Ser-94 and Ser-177, that are located within the actin-binding domain of spinophilin. Phosphorylation of spinophilin by PKA modulated the association between spinophilin and the actin cytoskeleton. Following subcellular fractionation, unphosphorylated spinophilin was enriched in the postsynaptic density, whereas a pool of phosphorylated spinophilin was found in the cytosol. F-actin co-sedimentation and overlay analysis revealed that phosphorylation of spinophilin reduced the stoichiometry of the spinophilin-actin interaction. In contrast, the ability of spinophilin to bind to PP1 remained unchanged. Taken together, our studies suggest that phosphorylation of spinophilin by PKA modulates the anchoring of the spinophilin-PP1 complex within dendritic spines, thereby likely contributing to the efficacy and plasticity of synaptic transmission

Study of HST counterparts to Chandra X-ray sources in the Globular Cluster M71

We report on archival Hubble Space Telescope (HST) observations of the globular cluster M71 (NGC 6838). These observations, covering the core of the globular cluster, were performed by the Advanced Camera for Surveys (ACS) and the Wide Field Planetary Camera 2 (WFPC2). Inside the half-mass radius (r_h = 1.65') of M71, we find 33 candidate optical counterparts to 25 out of 29 Chandra X-ray sources while outside the half-mass radius, 6 possible optical counterparts to 4 X-ray sources are found. Based on the X-ray and optical properties of the identifications, we find 1 certain and 7 candidate cataclysmic variables (CVs). We also classify 2 and 12 X-ray sources as certain and potential chromospherically active binaries (ABs), respectively. The only star in the error circle of the known millisecond pulsar (MSP) is inconsistent with being the optical counterpart. The number of X-ray faint sources with L_x>4x10^{30} ergs/s (0.5-6.0 keV) found in M71 is higher than extrapolations from other clusters on the basis of either collision frequency or mass. Since the core density of M71 is relatively low, we suggest that those CVs and ABs are primordial in origin.Comment: 12 pages, 6 figures. Accepted for publication in Astronomy and Astrophysic

Identification of the Plasticity-Relevant Fucose-α(1−2)-Galactose Proteome from the Mouse Olfactory Bulb

Fucose-α(1−2)-galactose [Fucα(1−2)Gal] sugars have been implicated in the molecular mechanisms that underlie neuronal development, learning, and memory. However, an understanding of their precise roles has been hampered by a lack of information regarding Fucα(1−2)Gal glycoproteins. Here, we report the first proteomic studies of this plasticity-relevant epitope. We identify five classes of putative Fucα(1−2)Gal glycoproteins: cell adhesion molecules, ion channels and solute carriers/transporters, ATP-binding proteins, synaptic vesicle-associated proteins, and mitochondrial proteins. In addition, we show that Fucα(1−2)Gal glycoproteins are enriched in the developing mouse olfactory bulb (OB) and exhibit a distinct spatiotemporal expression that is consistent with the presence of a “glycocode” to help direct olfactory sensory neuron (OSN) axonal pathfinding. We find that expression of Fucα(1−2)Gal sugars in the OB is regulated by the α(1−2)fucosyltransferase FUT1. FUT1-deficient mice exhibit developmental defects, including fewer and smaller glomeruli and a thinner olfactory nerve layer, suggesting that fucosylation contributes to OB development. Our findings significantly expand the number of Fucα(1−2)Gal glycoproteins and provide new insights into the molecular mechanisms by which fucosyl sugars contribute to neuronal processes

HIV with contact-tracing: a case study in Approximate Bayesian Computation

Missing data is a recurrent issue in epidemiology where the infection process may be partially observed. Approximate Bayesian Computation, an alternative to data imputation methods such as Markov Chain Monte Carlo integration, is proposed for making inference in epidemiological models. It is a likelihood-free method that relies exclusively on numerical simulations. ABC consists in computing a distance between simulated and observed summary statistics and weighting the simulations according to this distance. We propose an original extension of ABC to path-valued summary statistics, corresponding to the cumulated number of detections as a function of time. For a standard compartmental model with Suceptible, Infectious and Recovered individuals (SIR), we show that the posterior distributions obtained with ABC and MCMC are similar. In a refined SIR model well-suited to the HIV contact-tracing data in Cuba, we perform a comparison between ABC with full and binned detection times. For the Cuban data, we evaluate the efficiency of the detection system and predict the evolution of the HIV-AIDS disease. In particular, the percentage of undetected infectious individuals is found to be of the order of 40%

Pair Analysis of Field Galaxies from the Red-Sequence Cluster Survey

We study the evolution of the number of close companions of similar luminosities per galaxy (Nc) by choosing a volume-limited subset of the photometric redshift catalog from the Red-Sequence Cluster Survey (RCS-1). The sample contains over 157,000 objects with a moderate redshift range of 0.25 < z < 0.8 and absolute magnitude in Rc (M_Rc) < -20. This is the largest sample used for pair evolution analysis, providing data over 9 redshift bins with about 17,500 galaxies in each. After applying incompleteness and projection corrections, Nc shows a clear evolution with redshift. The Nc value for the whole sample grows with redshift as (1+z)^m, where m = 2.83 +/- 0.33 in good agreement with N-body simulations in a LCDM cosmology. We also separate the sample into two different absolute magnitude bins: -25 < M_Rc < -21 and -21 < M_Rc < -20, and find that the brighter the absolute magnitude, the smaller the m value. Furthermore, we study the evolution of the pair fraction for different projected separation bins and different luminosities. We find that the m value becomes smaller for larger separation, and the pair fraction for the fainter luminosity bin has stronger evolution. We derive the major merger remnant fraction f_rem = 0.06, which implies that about 6% of galaxies with -25 < M_Rc < -20 have undergone major mergers since z = 0.8.Comment: ApJ, in pres

Surface Acoustic Wave Probing of Ceramic Bearing Balls

This work is a continuation of our effort to develop a nondestructive technique for the detection and characterization of surface and near surface defects in ceramic bearing balls. We reported earlier on a method for detecting and sizing submicron surface depressions using a scanning acoustic microscope[1]. Our present work deals with the detection and sizing of surface cracks in the ceramic bearing balls, a problem which requires knowledge of the surface wave reflection coefficient of the crack, either at a single frequency in the long wavelength regime or as a function of frequency in the short wavelength regime. For this purpose, we need to learn the characteristics of surface wave propagation on spherical surfaces, the scattering of the surface waves from the cracks, and we need to develop a method for exciting the surface wave. We present a detailed theory of surface wave propagation on spheres. The results indicate that an arc source focuses the surface acoustic waved in a manner similar to bulk acoustic waves focusing by spherical transducers. We will present the details of this self focusing behavior. A spherical cap transducer structure similar to a planar wedge transducer is proposed to excite the spherical surface waves. We will present the details of the design of the spherical cap transducer for efficient surface wave excitation